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mmu_circBirc6_360
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_360
mm39_Chr. Position ID:
chr17|74984160|74996358|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
65,66
Exon/intron offsets:
0,12052
Genomic length:
12198
Exon/intron sizes in nt:
2,14,146
Flanking exon/introns:
chr17:74977377-74984160|chr17:74996358-74997352
Splice length:
360
Other database IDs:
circBase: hsa_circ_0119852, circAtlas: hsa-BIRC6_0085, CIRCpedia: HSA_CIRCpedia_40936
Human Ortholog IDs:
Mature spliced Sequence:
TTCTGGACATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCTTTCTACAGAGAATGGTGAAGAAGAGGAAGATGAACAGTCAGAATGTCAAACTTCTGTTGGTACATTATTAGCTAAAATGAAGACCTGTGTCGATACCTACACCAACCGCCTAAGATCTAAAAGGGAAAATGTGAAAGCCGGAGTCAAGCCGGACGCGCCTGATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGACATCCAGAGGACTGCTG
60.18
AACAACAGCGGCACCATAGA
59.96
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGACTGCTGAGATCGTCCAC
60.18
AACAACAGCGGCACCATAGA
59.96
148
PCR template sequence:
GATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAAGTTCTGGACATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAAGCCAATCAAGTTCTGGACATGGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_360_ORF_1
1.1024
0.27454755
0.954498562
711
ORF sequence:
ATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCTTTCTACAGAGAATGGTGAAGAAGAGGAAGATGAACAGTCAGAATGTCAAACTTCTGTTGGTACATTATTAGCTAAAATGAAGACCTGTGTCGATACCTACACCAACCGCCTAAGATCTAAAAGGGAAAATGTGAAAGCCGGAGTCAAGCCGGACGCGCCTGATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAAGTTCTGGACATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCTTTCTACAGAGAATGGTGAAGAAGAGGAAGATGAACAGTCAGAATGTCAAACTTCTGTTGGTACATTATTAGCTAAAATGAAGACCTGTGTCGATACCTACACCAACCGCCTAAGATCTAAAAGGGAAAATGTGAAAGCCGGAGTCAAGCCGGACGCGCCTGATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAA
Peptide sequence:
MARHVPLYRALLELLRAIASCTSMVPLLLPLSTENGEEEEDEQSECQTSVGTLLAKMKTCVDTYTNRLRSKRENVKAGVKPDAPDQEPEGLALLVPDIQRTAEIVHAATANLRQANQVLDMARHVPLYRALLELLRAIASCTSMVPLLLPLSTENGEEEEDEQSECQTSVGTLLAKMKTCVDTYTNRLRSKRENVKAGVKPDAPDQEPEGLALLVPDIQRTAEIVHAATANLRQANQ
IRESfinder result
IRESfinder score
0.24327243
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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