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mmu_circBirc6_2249
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_2249
mm39_Chr. Position ID:
chr17|74955112|74967677|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
53,54,55,56,57,58,59,60,61
Exon/intron offsets:
0,1505,3790,4499,6271,7054,10060,11041,12264
Genomic length:
12565
Exon/intron sizes in nt:
22,27,81,14,41,30,13,20,00,00,00,00,000
Flanking exon/introns:
chr17:74954820-74955112|chr17:74967677-74969595
Splice length:
2249
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AATTCAGGCCTTGCTAAAGTGGGTCAGTGACTCTGCAAAAATGGCTGCTTTGAAGAGAAGTGGCAGAATGAACTATATGTGCCCGAGCTCATCAGCTGTCGAGTATGGTCTTCTCATGCCATCCCCCTCTCATTTGCACTGTGTAGCAGCGATTCTTTGGCACAGTTATGAACTGCTGGTGGAGTATGATTTGCCAGCACTGCTAGACCGAGAGCTCTTTGAGTTACTTTTTAATTGGTCCATGTCACTTCCATGCAACGTGGTTTTGAAGAAAGCTGTTGACAGTCTGCTTTGCTCCATGTGCCATATACATCCAAATTATTTTTCCTTGCTCATGGGCTGGATGGGAATTATCCCTCCTCCGGTGCAGTGTCATCATAGACTATCCATGACAGATGATAGCAAGAAGCAAGATCTTAGTTCATCATTGACAGATGACTCCAAAAATGCACAAGCACCTCTGTCTCTAACTGAATCCCACTTGGCAACTCTTGCTTCTTCTTCTCAGTCCCCAGAAGCTATCAAACAGTTGCTAGACTCGGGGTTGCCTTCCCTTCTCGTGAGGAGTCTGGCTAGCTTTTGTTTTAGTCACATTTCTTACTCAGAAAGCATTGCCCAGTCAGTAGATAATTCCCAGGACAAACTCAGGCGGCATCACGTTCCACAGCACTGTAATAAGATGCCCATCACAGCAGACCTAGTGGCTCCCATCCTTAGGTTTTTGACAGAAGTTGGCAATAGTCATATCATGAAAGATTGGCTTGGTGGTTCTGAAGTCAACCCACTGTGGACAGCACTTCTGTTTTTGTTGTGTCACTCTGGGTCTACCGCTGGAGGACATAATTTAGGGGCACAGCAGAGCAGCACAAGGTCAGCTTCTCATTCTTCAGCTACTACAACAGTCCTGACCACCCAGCAACGGACAGCCATTGAGAACGCAACTGTTGCCTTCTTTCTGCAGTGTATTTCATGTCACCCCAATAACCAAAAGCTGATGGCACAGGTTCTCTGTGAACTGTTTCAGACAGCCCCTCAAAGGGGTAGCCTTCCAACATCTGGGAATATTTCCGGGTTTGTGCGAAGACTGTTTCTACAGTTGATGCTGGAGGATGAGAAGGTGACAATGTTTCTTCAGTCACCTTGCCCACTATATAAAGGAAGAATTAATGCAACTAGCCATGTCATCCAACACCCAATGTTTGGAGCAGGCCATAAGTTTCGTACTCTGCATTTGCCAGTCTCAACAACGTTGTCAGATGTGCTTGATAGAGTTTCAGATACACCAAGTATCACAGCTAAACTTATTAGTGAGCAAAAAGATGATAAAGAAAAGAAGAACCATGAAGAGAAAGAAAAAGTTAAGGCGGAAAATGGATTTCAGGATAACTACAGCGTTGTTGTTGCCTCTGGGCTGAAATCACAGTCTAAGCGTGCCATGGCATCCACACCACCTCGCCCACCATCCAGGAGAGGAAGAACCATTCCCGATAAGATCGGAAGTGCATCCTCAAGCGCAGACGCGGCCAGCAAAATCATCACCGTGCCCGTGTTTCATCTGTTCCACAGACTCCTGGCAGGTCAGCCACTACCAGCAGAAATGACACTTGCCCAGCTTTTAACTCTCCTCTATGACCGAAAACTTCCTCAAGGTTACCGTTCAATAGATCTTACTGTTAAATTGGGATCAAAAGTCATTACAGATCCAAGTTTGTCAAAAACAGATTCTTTCAAAAGACTGCACCCTGAAAAAGATCATGGAGACTTAGTTGGCAGCTGTCCAGAGGATGAGGCTCTTACCCCGAGTGATGAGTGCATGGATGGAGTATTGGATGAATCTTTGCTGGAAACCTGTCCTATTCAGTCACCATTACAAGTTTTTGCAGGAATGGGTGGACTGGCTCTTATTGCAGAGAGACTGCCCATGCTCTATCCAGAAGTGATTCAGCAGGTGAGTGCTCCAGTGATCGCTTCCACCACTCAGGAAAAGCCAAAGGATAGTGATCAGTTTGAATGGGTGACCATAGAACAATCAGGTGAACTGGTTTATGAAGCTCCAGAAACCATTGCAGCTGAGCCTCCACCTGTTAAGTCAGCGGTGCAAGCCACGTCTCCCATACCTGCCCATTCTCTGGCTGCTTTTGGATTATTTCTTCGTCTTCCGGGCTATGCTGAAGTGCTCCTGAAAGAGAGGAAGCATGCCCAGTGCCTTCTTCGATTGGTGTTGGGAGTTACAGATGATGGAGAAGGCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGAGAGGAAGCATGCCCAG
59.74
CTGATGAGCTCGGGCACATA
59.61
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCAGTGCCTTCTTCGATT
60.68
CTGATGAGCTCGGGCACATA
59.61
147
PCR template sequence:
CTTCGTCTTCCGGGCTATGCTGAAGTGCTCCTGAAAGAGAGGAAGCATGCCCAGTGCCTTCTTCGATTGGTGTTGGGAGTTACAGATGATGGAGAAGGCAAATTCAGGCCTTGCTAAAGTGGGTCAGTGACTCTGCAAAAATGGCTGCTTTGAAGAGAAGTGGCAGAATGAACTATATGTGCCCGAGCTCATCAGCTGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(2), mmu-miR-134-5p(1), mmu-miR-150-5p(1), mmu-miR-135a-5p(1), mmu-miR-130a-3p(1), mmu-miR-132-3p(1), mmu-miR-320-3p(1), mmu-miR-22-3p(1), mmu-miR-212-3p(1), mmu-miR-130b-3p(1), mmu-miR-721(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-452-3p(1), mmu-miR-3065-5p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATGGAGAAGGCAAATTCAGGCCTTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_2249_ORF_1
0.9073
0.055147476
0.999996721
2226
ORF sequence:
ATGGCTGCTTTGAAGAGAAGTGGCAGAATGAACTATATGTGCCCGAGCTCATCAGCTGTCGAGTATGGTCTTCTCATGCCATCCCCCTCTCATTTGCACTGTGTAGCAGCGATTCTTTGGCACAGTTATGAACTGCTGGTGGAGTATGATTTGCCAGCACTGCTAGACCGAGAGCTCTTTGAGTTACTTTTTAATTGGTCCATGTCACTTCCATGCAACGTGGTTTTGAAGAAAGCTGTTGACAGTCTGCTTTGCTCCATGTGCCATATACATCCAAATTATTTTTCCTTGCTCATGGGCTGGATGGGAATTATCCCTCCTCCGGTGCAGTGTCATCATAGACTATCCATGACAGATGATAGCAAGAAGCAAGATCTTAGTTCATCATTGACAGATGACTCCAAAAATGCACAAGCACCTCTGTCTCTAACTGAATCCCACTTGGCAACTCTTGCTTCTTCTTCTCAGTCCCCAGAAGCTATCAAACAGTTGCTAGACTCGGGGTTGCCTTCCCTTCTCGTGAGGAGTCTGGCTAGCTTTTGTTTTAGTCACATTTCTTACTCAGAAAGCATTGCCCAGTCAGTAGATAATTCCCAGGACAAACTCAGGCGGCATCACGTTCCACAGCACTGTAATAAGATGCCCATCACAGCAGACCTAGTGGCTCCCATCCTTAGGTTTTTGACAGAAGTTGGCAATAGTCATATCATGAAAGATTGGCTTGGTGGTTCTGAAGTCAACCCACTGTGGACAGCACTTCTGTTTTTGTTGTGTCACTCTGGGTCTACCGCTGGAGGACATAATTTAGGGGCACAGCAGAGCAGCACAAGGTCAGCTTCTCATTCTTCAGCTACTACAACAGTCCTGACCACCCAGCAACGGACAGCCATTGAGAACGCAACTGTTGCCTTCTTTCTGCAGTGTATTTCATGTCACCCCAATAACCAAAAGCTGATGGCACAGGTTCTCTGTGAACTGTTTCAGACAGCCCCTCAAAGGGGTAGCCTTCCAACATCTGGGAATATTTCCGGGTTTGTGCGAAGACTGTTTCTACAGTTGATGCTGGAGGATGAGAAGGTGACAATGTTTCTTCAGTCACCTTGCCCACTATATAAAGGAAGAATTAATGCAACTAGCCATGTCATCCAACACCCAATGTTTGGAGCAGGCCATAAGTTTCGTACTCTGCATTTGCCAGTCTCAACAACGTTGTCAGATGTGCTTGATAGAGTTTCAGATACACCAAGTATCACAGCTAAACTTATTAGTGAGCAAAAAGATGATAAAGAAAAGAAGAACCATGAAGAGAAAGAAAAAGTTAAGGCGGAAAATGGATTTCAGGATAACTACAGCGTTGTTGTTGCCTCTGGGCTGAAATCACAGTCTAAGCGTGCCATGGCATCCACACCACCTCGCCCACCATCCAGGAGAGGAAGAACCATTCCCGATAAGATCGGAAGTGCATCCTCAAGCGCAGACGCGGCCAGCAAAATCATCACCGTGCCCGTGTTTCATCTGTTCCACAGACTCCTGGCAGGTCAGCCACTACCAGCAGAAATGACACTTGCCCAGCTTTTAACTCTCCTCTATGACCGAAAACTTCCTCAAGGTTACCGTTCAATAGATCTTACTGTTAAATTGGGATCAAAAGTCATTACAGATCCAAGTTTGTCAAAAACAGATTCTTTCAAAAGACTGCACCCTGAAAAAGATCATGGAGACTTAGTTGGCAGCTGTCCAGAGGATGAGGCTCTTACCCCGAGTGATGAGTGCATGGATGGAGTATTGGATGAATCTTTGCTGGAAACCTGTCCTATTCAGTCACCATTACAAGTTTTTGCAGGAATGGGTGGACTGGCTCTTATTGCAGAGAGACTGCCCATGCTCTATCCAGAAGTGATTCAGCAGGTGAGTGCTCCAGTGATCGCTTCCACCACTCAGGAAAAGCCAAAGGATAGTGATCAGTTTGAATGGGTGACCATAGAACAATCAGGTGAACTGGTTTATGAAGCTCCAGAAACCATTGCAGCTGAGCCTCCACCTGTTAAGTCAGCGGTGCAAGCCACGTCTCCCATACCTGCCCATTCTCTGGCTGCTTTTGGATTATTTCTTCGTCTTCCGGGCTATGCTGAAGTGCTCCTGAAAGAGAGGAAGCATGCCCAGTGCCTTCTTCGATTGGTGTTGGGAGTTACAGATGATGGAGAAGGCAAATTCAGGCCTTGCTAA
Peptide sequence:
MAALKRSGRMNYMCPSSSAVEYGLLMPSPSHLHCVAAILWHSYELLVEYDLPALLDRELFELLFNWSMSLPCNVVLKKAVDSLLCSMCHIHPNYFSLLMGWMGIIPPPVQCHHRLSMTDDSKKQDLSSSLTDDSKNAQAPLSLTESHLATLASSSQSPEAIKQLLDSGLPSLLVRSLASFCFSHISYSESIAQSVDNSQDKLRRHHVPQHCNKMPITADLVAPILRFLTEVGNSHIMKDWLGGSEVNPLWTALLFLLCHSGSTAGGHNLGAQQSSTRSASHSSATTTVLTTQQRTAIENATVAFFLQCISCHPNNQKLMAQVLCELFQTAPQRGSLPTSGNISGFVRRLFLQLMLEDEKVTMFLQSPCPLYKGRINATSHVIQHPMFGAGHKFRTLHLPVSTTLSDVLDRVSDTPSITAKLISEQKDDKEKKNHEEKEKVKAENGFQDNYSVVVASGLKSQSKRAMASTPPRPPSRRGRTIPDKIGSASSSADAASKIITVPVFHLFHRLLAGQPLPAEMTLAQLLTLLYDRKLPQGYRSIDLTVKLGSKVITDPSLSKTDSFKRLHPEKDHGDLVGSCPEDEALTPSDECMDGVLDESLLETCPIQSPLQVFAGMGGLALIAERLPMLYPEVIQQVSAPVIASTTQEKPKDSDQFEWVTIEQSGELVYEAPETIAAEPPPVKSAVQATSPIPAHSLAAFGLFLRLPGYAEVLLKERKHAQCLLRLVLGVTDDGEGKFRPC
IRESfinder result
IRESfinder score
0.640977667
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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