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mmu_circBirc6_1467
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_1467
mm39_Chr. Position ID:
chr17|74892947|74906962|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
10,11,12,13,14,15,16,17,18,19
Exon/intron offsets:
0,3642,7136,8411,8917,10904,12086,13030,13284,13917
Genomic length:
14015
Exon/intron sizes in nt:
150,226,161,90,132,182,134,162,132,98
Flanking exon/introns:
chr17:74887557-74892947|chr17:74906962-74909959
Splice length:
1467
Other database IDs:
circAtlas: mmu-Birc6_0015
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_369788
Human Ortholog IDs:
Mature spliced Sequence:
GTGGTGAGCTTCATTTTCTCCAAATTGGAGGAACCTGTGATGATATTGATGAAGCTGATATTCTGGTAGATGGATCCCTTTCTAAAGGAATAGAACCAGCTCTAGAAGGTTCCAGACCTTTATCAAATCCTTCAAGTCCTGGTATTTCAGGAGTTGAGTTATTGGTGGACCAGCCTTTCACACTTGAAATCTTGACGTCTTTAGTAGAACTAACTCGATTTGAAACACTGACTCCACGATTTTCAGCAACTGTTCCTCCATGTTGGGTAGAAGTTCAGCAAGAACAGCAGCAAAGAAGGCACCCGCAGCATCTGCACCAGCAGCACCACGGCGATGCTGCTCAGCACACCCGGACCTGGAAACTGCAGACCGACAGCAACAGCTGGGATGAACATGTGTTTGAATTGGTTCTACCTAAAGCCTGTATGGTTGGACATGTGGACTTCAAATTCGTTCTGAACTCCAACATCACCAGTGTCCCCCAGATACAAGTGACACTGCTGAAGAACAAAGCTCCAGGCCTAGGGAAAGCCAATGCTCTTAACATTGAAGTGGAACATAATGGGAACCCGTCCCTGGTTGATTTGAATGAAGAAATGCACCACATGGATGTAGAGGAGTCACAGTGTCTTAGGTTATGTCCATTCTTAGAGGATCATAAAGAAGACATTCTGTGTGGCCCAGTATGGCTTGCTAGTGGTCTGGACCTATCAGGTCATGCTGGGATGTTGACGTTGACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGGAAGTACCGTTCATTTTTAATTCATGTCAAAGCAGTGAGTGACCGAGGAGCAGCAGATGAGATGTGCAGCAGTGGCCTGCGTCCTGTTGTGAGGCTTCCATCTCTTAAACAGCAGGGTCACAAGGGATACTCACTGGCTTCTCTCTTGGCCAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATGTTAAAAATGAAAATGCAGGAGGCACCCGAAAGTCTGAAAACCTGCGGGGTTGTGATTTACTTCAGGAGGTGTCTGTCACCATTCGGAGGTTTAAGAAAACCTCCATCTGTAAGGAAAGGGTTCAGCGTTGCGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTCTTAACACGCTTTGTAGACGATCAGATGATGGGCAGGTCACAGAGCACGCCCAGAGTCTTGTGTTGGATGCTCTCTGCTGGTTAGCAGGAGTCCATTCAAATGGATCTGGCAGCTCAAAGGAAGGAAATGAGTGCCTACTTTCAAAAACACGGAAATGTCTTTCCGATATTGTCCGTGTATGCTTCTTTGAGGCAGGACGAAGTATAGCCCATAAATGTGCTCGGTTTCTAGCCTTGTGCATTAGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCTGGATTTGGATCGGT
60.11
AGAAAGGGATCCATCTACCAGA
57.71
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGCCTGGATTTGGATCGG
59.82
AGAAAGGGATCCATCTACCAGA
57.71
160
PCR template sequence:
AGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGTGGTGGTGAGCTTCATTTTCTCCAAATTGGAGGAACCTGTGATGATATTGATGAAGCTGATATTCTGGTAGATGGATCCCTTTCTAAAGGAATAGAACCAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-26a-5p(1), mmu-miR-15b-5p(1), mmu-miR-26b-5p(1), mmu-miR-22-3p(1), mmu-miR-127-5p(1), mmu-miR-541-5p(1), mmu-miR-107-3p(1), mmu-miR-690(1), mmu-miR-335-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAGCAACTGGTGGTGGTGAGCTTCA
siRNA sets
TGAAGCTCACCACCACCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_1467_ORF_1
0.8228
0.06829238
0.999999542
2508
ORF sequence:
ATGGTTGGACATGTGGACTTCAAATTCGTTCTGAACTCCAACATCACCAGTGTCCCCCAGATACAAGTGACACTGCTGAAGAACAAAGCTCCAGGCCTAGGGAAAGCCAATGCTCTTAACATTGAAGTGGAACATAATGGGAACCCGTCCCTGGTTGATTTGAATGAAGAAATGCACCACATGGATGTAGAGGAGTCACAGTGTCTTAGGTTATGTCCATTCTTAGAGGATCATAAAGAAGACATTCTGTGTGGCCCAGTATGGCTTGCTAGTGGTCTGGACCTATCAGGTCATGCTGGGATGTTGACGTTGACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGGAAGTACCGTTCATTTTTAATTCATGTCAAAGCAGTGAGTGACCGAGGAGCAGCAGATGAGATGTGCAGCAGTGGCCTGCGTCCTGTTGTGAGGCTTCCATCTCTTAAACAGCAGGGTCACAAGGGATACTCACTGGCTTCTCTCTTGGCCAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATGTTAAAAATGAAAATGCAGGAGGCACCCGAAAGTCTGAAAACCTGCGGGGTTGTGATTTACTTCAGGAGGTGTCTGTCACCATTCGGAGGTTTAAGAAAACCTCCATCTGTAAGGAAAGGGTTCAGCGTTGCGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTCTTAACACGCTTTGTAGACGATCAGATGATGGGCAGGTCACAGAGCACGCCCAGAGTCTTGTGTTGGATGCTCTCTGCTGGTTAGCAGGAGTCCATTCAAATGGATCTGGCAGCTCAAAGGAAGGAAATGAGTGCCTACTTTCAAAAACACGGAAATGTCTTTCCGATATTGTCCGTGTATGCTTCTTTGAGGCAGGACGAAGTATAGCCCATAAATGTGCTCGGTTTCTAGCCTTGTGCATTAGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGTGGTGGTGAGCTTCATTTTCTCCAAATTGGAGGAACCTGTGATGATATTGATGAAGCTGATATTCTGGTAGATGGATCCCTTTCTAAAGGAATAGAACCAGCTCTAGAAGGTTCCAGACCTTTATCAAATCCTTCAAGTCCTGGTATTTCAGGAGTTGAGTTATTGGTGGACCAGCCTTTCACACTTGAAATCTTGACGTCTTTAGTAGAACTAACTCGATTTGAAACACTGACTCCACGATTTTCAGCAACTGTTCCTCCATGTTGGGTAGAAGTTCAGCAAGAACAGCAGCAAAGAAGGCACCCGCAGCATCTGCACCAGCAGCACCACGGCGATGCTGCTCAGCACACCCGGACCTGGAAACTGCAGACCGACAGCAACAGCTGGGATGAACATGTGTTTGAATTGGTTCTACCTAAAGCCTGTATGGTTGGACATGTGGACTTCAAATTCGTTCTGAACTCCAACATCACCAGTGTCCCCCAGATACAAGTGACACTGCTGAAGAACAAAGCTCCAGGCCTAGGGAAAGCCAATGCTCTTAACATTGAAGTGGAACATAATGGGAACCCGTCCCTGGTTGATTTGAATGAAGAAATGCACCACATGGATGTAGAGGAGTCACAGTGTCTTAGGTTATGTCCATTCTTAGAGGATCATAAAGAAGACATTCTGTGTGGCCCAGTATGGCTTGCTAGTGGTCTGGACCTATCAGGTCATGCTGGGATGTTGACGTTGACAAGCCCCAAACTTGTTAAAGGTATGGCAGGAGGGAAGTACCGTTCATTTTTAATTCATGTCAAAGCAGTGAGTGACCGAGGAGCAGCAGATGAGATGTGCAGCAGTGGCCTGCGTCCTGTTGTGAGGCTTCCATCTCTTAAACAGCAGGGTCACAAGGGATACTCACTGGCTTCTCTCTTGGCCAAAGTTGCAGCAGGCAAGGAAAAATCATCTAATGTTAAAAATGAAAATGCAGGAGGCACCCGAAAGTCTGAAAACCTGCGGGGTTGTGATTTACTTCAGGAGGTGTCTGTCACCATTCGGAGGTTTAAGAAAACCTCCATCTGTAAGGAAAGGGTTCAGCGTTGCGCCATGTTACAGTTTTCAGAATTTCATGAGAAGCTTCTTAACACGCTTTGTAGACGATCAGATGATGGGCAGGTCACAGAGCACGCCCAGAGTCTTGTGTTGGATGCTCTCTGCTGGTTAGCAGGAGTCCATTCAAATGGATCTGGCAGCTCAAAGGAAGGAAATGAGTGCCTACTTTCAAAAACACGGAAATGTCTTTCCGATATTGTCCGTGTATGCTTCTTTGAGGCAGGACGAAGTATAGCCCATAAATGTGCTCGGTTTCTAGCCTTGTGCATTAGTAATGGCAAATGCGAGCCCTGTCAGCCTGGATTTGGATCGGTTCTATTGAAGGCTTTACTTGATAATATGTGCTTTTTACCAGCAGCAGCAACTGGT
Peptide sequence:
MVGHVDFKFVLNSNITSVPQIQVTLLKNKAPGLGKANALNIEVEHNGNPSLVDLNEEMHHMDVEESQCLRLCPFLEDHKEDILCGPVWLASGLDLSGHAGMLTLTSPKLVKGMAGGKYRSFLIHVKAVSDRGAADEMCSSGLRPVVRLPSLKQQGHKGYSLASLLAKVAAGKEKSSNVKNENAGGTRKSENLRGCDLLQEVSVTIRRFKKTSICKERVQRCAMLQFSEFHEKLLNTLCRRSDDGQVTEHAQSLVLDALCWLAGVHSNGSGSSKEGNECLLSKTRKCLSDIVRVCFFEAGRSIAHKCARFLALCISNGKCEPCQPGFGSVLLKALLDNMCFLPAAATGGGELHFLQIGGTCDDIDEADILVDGSLSKGIEPALEGSRPLSNPSSPGISGVELLVDQPFTLEILTSLVELTRFETLTPRFSATVPPCWVEVQQEQQQRRHPQHLHQQHHGDAAQHTRTWKLQTDSNSWDEHVFELVLPKACMVGHVDFKFVLNSNITSVPQIQVTLLKNKAPGLGKANALNIEVEHNGNPSLVDLNEEMHHMDVEESQCLRLCPFLEDHKEDILCGPVWLASGLDLSGHAGMLTLTSPKLVKGMAGGKYRSFLIHVKAVSDRGAADEMCSSGLRPVVRLPSLKQQGHKGYSLASLLAKVAAGKEKSSNVKNENAGGTRKSENLRGCDLLQEVSVTIRRFKKTSICKERVQRCAMLQFSEFHEKLLNTLCRRSDDGQVTEHAQSLVLDALCWLAGVHSNGSGSSKEGNECLLSKTRKCLSDIVRVCFFEAGRSIAHKCARFLALCISNGKCEPCQPGFGSVLLKALLDNMCFLPAAATG
IRESfinder result
IRESfinder score
0.664805368
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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