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mmu_circBfar_377
Basic information of circular RNA
PanCircBase ID:
mmu_circBfar_377
mm39_Chr. Position ID:
chr16|13515196|13516939|+
Gene name:
Bfar
Transcript:
ENSMUST00000023365.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
6,7
Exon/intron offsets:
0,1540
Genomic length:
1743
Exon/intron sizes in nt:
174,203
Flanking exon/introns:
chr16:13510441-13515196|chr16:13516939-13519928
Splice length:
377
Other database IDs:
circBase: mmu_circ_0000663, CIRCpedia: MMU_CIRCpedia_8487, circAtlas: mmu-Bfar_0002
circAtlas: hsa-BFAR_0002, CIRCpedia: HSA_CIRCpedia_16451
Human Ortholog IDs:
Mature spliced Sequence:
GCTGTCAACCCGGGTAGATCCCTATTCTTGCTCTACGCTCTCAAGAGCTCACCCAGACTTGGCTTGCTATACCTGTACCTGTTTGACTACACAGACTGCTTCCTACCCTTCATCCACACCATCTGCCCTCTGCAGGAAAACAGCTCTGGCGAGGACATTTTCACCAAGCTTCTGGACCTGAGGGAGCCCACGTGGAAGCAGTGGAGAGAATTCCTCGTCAAGTACTCCTTTCTTCCGTACCAGCTGATCGCGGAGTTTGCCTGGGACTGGCTGGAGGTCCACTACTGGACATCTCGCTTCCTGATCGTCAATGCCGTGCTGCTCTCTGTTCTGGAGTTGTTCTCTTTTTGGAGGATCTGGTCGAGAAGTGAGCTGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTGATCGTCAATGCCGT
59.75
CCAAGTCTGGGTGAGCTCTT
59.31
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCTTCCTGATCGTCAATGC
59.7
CCAAGTCTGGGTGAGCTCTT
59.31
145
PCR template sequence:
TCCACTACTGGACATCTCGCTTCCTGATCGTCAATGCCGTGCTGCTCTCTGTTCTGGAGTTGTTCTCTTTTTGGAGGATCTGGTCGAGAAGTGAGCTGAAGCTGTCAACCCGGGTAGATCCCTATTCTTGCTCTACGCTCTCAAGAGCTCACCCAGACTTGGCTTGCTATACCTGTACCTGTTTGACTACACAGACTGCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15b-5p(1), mmu-miR-298-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGTGAGCTGAAGCTGTCAACCCGG
siRNA sets
TTGACAGCTTCAGCTCACT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.760333117
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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