top of page
mmu_circBbx_2203
Basic information of circular RNA
PanCircBase ID:
mmu_circBbx_2203
mm39_Chr. Position ID:
chr16|50040807|50100902|-
Gene name:
Bbx
Transcript:
ENSMUST00000138166.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,2805,3843,20409,27337,30869,41971,45732,54102,59924
Genomic length:
60095
Exon/intron sizes in nt:
14,91,36,10,06,81,75,80,00,00,00,00,000
Flanking exon/introns:
chr16:50029507-50040807|chr16:50100902-50151391
Splice length:
2203
Other database IDs:
circAtlas: mmu-Bbx_0014
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTCACAGTAATGAAAGGCAGTAATAGAAATAAAGACCATTCAACAGAAGGAGAAGGGGACGGAAAACGACCAAAACGGAAGTGTCTTCAGTGGCATCCGTTGCTAGCTAAGAAACTTCTGGACTTTTCAGAGGAGGAGGAGGAAGACGAGGAAGAGGAAGATATTGATAAAGTTCAACTTCTTGAGGCGGATGGCCTAGAGCAAGATGTTGCTGAGACTGAAGATGATGAGTCACCTGAGCAGCGAGCCCGGAGACCAATGAATGCATTTCTTTTATTTTGCAAGCGCCACCGCTCCCTTGTCCGACAGGAACACCCAAGGCTTGATAACCGAGGTGCTACCAAGATACTAGCTGATTGGTGGGCTGTTCTTGATCCTAAGGAAAAGCAGAAATATACAGACATGGCCAAGGAGTATAAAGATGCATTTATGAAAGCAAATCCTGGCTACAGATGGTGCCCTACCACAAACAAGCCTGTGAAATCGCCAACACCCACTGTCAATCCACGGAAGAAACTTTGGGCCTTCCCACCTGACTCTTCAAGAGACTTGCCAACCCCCAAGAAGGCAAAGACAGAAGTTCCTCAGCTCAACTTTGGAATGGCAGATCCTACTCAGATGGGGGGCCTGAGCATGTTGCTGCTAGCTGGAGAGCACGCTCTTGGGACACCAGAGGCATCCTCTGGGACTTGCAGGCCTGATATTTCAGAATCACCTGAGTTGCGTCAGAAGTCACCATTGTTTCAGTTTGCCGAGATATCTTCAAGGACATCGCACCCCGATGCGCCTTCAAAACAGTGTCAAGCGTCAGCCTTGTTTCAGTTTGCAGAGATCTCTTCAAGCACTTCGCAGTTGGGTGGCACTGAGCCTGTGAAACGCTGTGGAAATTCTGCACTCTTTCAACTGGCAGAGATGTGTCTGGCATCAGAGGGGGTGAAAATGGAAGACACAAAACTAATAAAATCAAAAGAGTCAGATGGAGGAAGAATTGAGGAAATAGAAAAGGGGAAGGAAGAGAGAGGCACAGAAGTGGAGAAAACCACTGAAACTTCGTTCCAGAAGGAAGCAGAATTTGGGAAGTCAGCTAAGGGCAACGTGAGAGAGTCTAAGGACTTAAGAGACATCGAACAGCTACAGATGGATAATGTCATGGCTATCAAAGTGGAAGACCCCAAAGAAATTAGGAAGGAGCCAGAGGATGATCAGAAATACAGTCACTTCCCTGATTTTTCTTACTCTGCCAGTAGCAAGATAATAATTAGTGGTGTCCCCAGTAGAAAGGACCACATGTGCCATCCTCACGGAATTATGATCATTGAGGACCCAACCACGCTAAACAAACCAGAGAAGATAAAGAAGAAAAAGAAGAAAAACAAACTGGATCGACACGGAAATGACAAATCCACACCCAAGAAGACGTGCAAGAAGAGGCAGTCTTCAGAATCTGACATCGAGAGTGTCATGTACACCATCGAAGCTGTGGCAAAAGGAGACTGGGGTGTTGACAAACTCGGAGAAACCCCAAGAAAGAAAGTCCGCCCCTCCTCAAGTGGCAAGGGAGGCATTTTGGATGCCAAGCCACCAAAGAAAAAAGTGAAATCCAAGGAGAAGAAAGTTTCCAAGGAGAAGTGCTCAGACATCACCAAAGAGTCTCGACCTCCAGATTTCCTTAGCATTTCTGCCAGCAAGAGTGTTCCTGGGGAGGTGCCAGAGGGCATAAAAGCAGAACCCTTGACCCCTACCGAGGATGCACTGCCACCTAGCCTACCAGGACAGGCTAAGCCTGAGGATAGTGATTGTCACAGAAAAACGGAGACCTGTGGCTCCAGGAAATCTGAGAGGTCTTGCAAAGGTGCTCTTTATAAAACCCTGGTGTCCGAGGGCATGCTCACCTCTCTCCGAGCTAATGTTGACAGAGGGAAACGAAGCTCAGGAAAAGGAAACTCCTCTGATCATGAAGGGTGTTGGAGTGAAGAGAGCTGGACATTTAATCAGAGTGGGACCAGTGGGAGCAAGAAGTTTAAGAAGAAACTAAGGGAAGACTCCTTCCTTGGTTCTGCGAAGCTGGATGAAGAATTTGAAAAGAAATTCAACAGCCTCCCTCAATATAGTCCTATTACATTTGACCGGAAATGCGTATCTACTCCAAGAAAAAAGAAGAAGACTGGAAACATGTCCTCAGAATCGACTAAAACCAGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATTTGACCGGAAATGCGT
58.76
GTCGTTTTCCGTCCCCTTCT
59.97
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACATTTGACCGGAAATGCGT
58.76
TTCCGTCCCCTTCTCCTTCT
59.88
149
PCR template sequence:
CAATATAGTCCTATTACATTTGACCGGAAATGCGTATCTACTCCAAGAAAAAAGAAGAAGACTGGAAACATGTCCTCAGAATCGACTAAAACCAGCAAAGGTCACAGTAATGAAAGGCAGTAATAGAAATAAAGACCATTCAACAGAAGGAGAAGGGGACGGAAAACGACCAAAACGGAAGTGTCTTCAGTGGCATCCGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
21
Interacting miRNAs (#binding sites)
mmu-miR-1904(2), mmu-miR-9-5p(1), mmu-miR-140-5p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-148b-3p(1), mmu-miR-18a-5p(1), mmu-miR-24-3p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-342-5p(1), mmu-miR-207(1), mmu-miR-484(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAACCAGCAAAGGTCACAGTAATGA
siRNA sets
ATTACTGTGACCTTTGCTG, TTACTGTGACCTTTGCTGG, CATTACTGTGACCTTTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBBX_2203_ORF_1
0.8934
0.203248919
0.999999177
2337
ORF sequence:
ATGAAGAATTTGAAAAGAAATTCAACAGCCTCCCTCAATATAGTCCTATTACATTTGACCGGAAATGCGTATCTACTCCAAGAAAAAAGAAGAAGACTGGAAACATGTCCTCAGAATCGACTAAAACCAGCAAAGGTCACAGTAATGAAAGGCAGTAATAGAAATAAAGACCATTCAACAGAAGGAGAAGGGGACGGAAAACGACCAAAACGGAAGTGTCTTCAGTGGCATCCGTTGCTAGCTAAGAAACTTCTGGACTTTTCAGAGGAGGAGGAGGAAGACGAGGAAGAGGAAGATATTGATAAAGTTCAACTTCTTGAGGCGGATGGCCTAGAGCAAGATGTTGCTGAGACTGAAGATGATGAGTCACCTGAGCAGCGAGCCCGGAGACCAATGAATGCATTTCTTTTATTTTGCAAGCGCCACCGCTCCCTTGTCCGACAGGAACACCCAAGGCTTGATAACCGAGGTGCTACCAAGATACTAGCTGATTGGTGGGCTGTTCTTGATCCTAAGGAAAAGCAGAAATATACAGACATGGCCAAGGAGTATAAAGATGCATTTATGAAAGCAAATCCTGGCTACAGATGGTGCCCTACCACAAACAAGCCTGTGAAATCGCCAACACCCACTGTCAATCCACGGAAGAAACTTTGGGCCTTCCCACCTGACTCTTCAAGAGACTTGCCAACCCCCAAGAAGGCAAAGACAGAAGTTCCTCAGCTCAACTTTGGAATGGCAGATCCTACTCAGATGGGGGGCCTGAGCATGTTGCTGCTAGCTGGAGAGCACGCTCTTGGGACACCAGAGGCATCCTCTGGGACTTGCAGGCCTGATATTTCAGAATCACCTGAGTTGCGTCAGAAGTCACCATTGTTTCAGTTTGCCGAGATATCTTCAAGGACATCGCACCCCGATGCGCCTTCAAAACAGTGTCAAGCGTCAGCCTTGTTTCAGTTTGCAGAGATCTCTTCAAGCACTTCGCAGTTGGGTGGCACTGAGCCTGTGAAACGCTGTGGAAATTCTGCACTCTTTCAACTGGCAGAGATGTGTCTGGCATCAGAGGGGGTGAAAATGGAAGACACAAAACTAATAAAATCAAAAGAGTCAGATGGAGGAAGAATTGAGGAAATAGAAAAGGGGAAGGAAGAGAGAGGCACAGAAGTGGAGAAAACCACTGAAACTTCGTTCCAGAAGGAAGCAGAATTTGGGAAGTCAGCTAAGGGCAACGTGAGAGAGTCTAAGGACTTAAGAGACATCGAACAGCTACAGATGGATAATGTCATGGCTATCAAAGTGGAAGACCCCAAAGAAATTAGGAAGGAGCCAGAGGATGATCAGAAATACAGTCACTTCCCTGATTTTTCTTACTCTGCCAGTAGCAAGATAATAATTAGTGGTGTCCCCAGTAGAAAGGACCACATGTGCCATCCTCACGGAATTATGATCATTGAGGACCCAACCACGCTAAACAAACCAGAGAAGATAAAGAAGAAAAAGAAGAAAAACAAACTGGATCGACACGGAAATGACAAATCCACACCCAAGAAGACGTGCAAGAAGAGGCAGTCTTCAGAATCTGACATCGAGAGTGTCATGTACACCATCGAAGCTGTGGCAAAAGGAGACTGGGGTGTTGACAAACTCGGAGAAACCCCAAGAAAGAAAGTCCGCCCCTCCTCAAGTGGCAAGGGAGGCATTTTGGATGCCAAGCCACCAAAGAAAAAAGTGAAATCCAAGGAGAAGAAAGTTTCCAAGGAGAAGTGCTCAGACATCACCAAAGAGTCTCGACCTCCAGATTTCCTTAGCATTTCTGCCAGCAAGAGTGTTCCTGGGGAGGTGCCAGAGGGCATAAAAGCAGAACCCTTGACCCCTACCGAGGATGCACTGCCACCTAGCCTACCAGGACAGGCTAAGCCTGAGGATAGTGATTGTCACAGAAAAACGGAGACCTGTGGCTCCAGGAAATCTGAGAGGTCTTGCAAAGGTGCTCTTTATAAAACCCTGGTGTCCGAGGGCATGCTCACCTCTCTCCGAGCTAATGTTGACAGAGGGAAACGAAGCTCAGGAAAAGGAAACTCCTCTGATCATGAAGGGTGTTGGAGTGAAGAGAGCTGGACATTTAATCAGAGTGGGACCAGTGGGAGCAAGAAGTTTAAGAAGAAACTAAGGGAAGACTCCTTCCTTGGTTCTGCGAAGCTGGATGAAGAATTTGAAAAGAAATTCAACAGCCTCCCTCAATATAGTCCTATTACATTTGACCGGAAATGCGTATCTACTCCAAGAAAAAAGAAGAAGACTGGAAACATGTCCTCAGAATCGACTAAAACCAGCAAA
Peptide sequence:
MKNLKRNSTASLNIVLLHLTGNAYLLQEKRRRLETCPQNRLKPAKVTVMKGSNRNKDHSTEGEGDGKRPKRKCLQWHPLLAKKLLDFSEEEEEDEEEEDIDKVQLLEADGLEQDVAETEDDESPEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAVLDPKEKQKYTDMAKEYKDAFMKANPGYRWCPTTNKPVKSPTPTVNPRKKLWAFPPDSSRDLPTPKKAKTEVPQLNFGMADPTQMGGLSMLLLAGEHALGTPEASSGTCRPDISESPELRQKSPLFQFAEISSRTSHPDAPSKQCQASALFQFAEISSSTSQLGGTEPVKRCGNSALFQLAEMCLASEGVKMEDTKLIKSKESDGGRIEEIEKGKEERGTEVEKTTETSFQKEAEFGKSAKGNVRESKDLRDIEQLQMDNVMAIKVEDPKEIRKEPEDDQKYSHFPDFSYSASSKIIISGVPSRKDHMCHPHGIMIIEDPTTLNKPEKIKKKKKKNKLDRHGNDKSTPKKTCKKRQSSESDIESVMYTIEAVAKGDWGVDKLGETPRKKVRPSSSGKGGILDAKPPKKKVKSKEKKVSKEKCSDITKESRPPDFLSISASKSVPGEVPEGIKAEPLTPTEDALPPSLPGQAKPEDSDCHRKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRSSGKGNSSDHEGCWSEESWTFNQSGTSGSKKFKKKLREDSFLGSAKLDEEFEKKFNSLPQYSPITFDRKCVSTPRKKKKTGNMSSESTKTSK
IRESfinder result
IRESfinder score
0.454397034
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page