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mmu_circBbs5_348
Basic information of circular RNA
PanCircBase ID:
mmu_circBbs5_348
mm39_Chr. Position ID:
chr2|69483125|69487336|+
Gene name:
Bbs5
Transcript:
ENSMUST00000134659.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,1281,2717,4115
Genomic length:
4211
Exon/intron sizes in nt:
66,50,136,96
Flanking exon/introns:
chr2:69479448-69483125|chr2:69487336-69491379
Splice length:
348
Other database IDs:
circAtlas: mmu-Bbs5_0009
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_122723
Human Ortholog IDs:
Mature spliced Sequence:
GTAGACTCTTAGTCACAAACTTAAGGATCATCTGGCATTCCTTGGCATTGCCCAGAGTCAATCTTTCTATTGGTTACAACTGCATATTGAATATCACAACAAGAACTGCTAACTCTAGCATATGAAACTTCTAAAATGTACCGTGACTTTAAGTTGAGAAGTGCAGTCATTCAGAACAAGCAGCTGAGGTTACTACCACAGGAGCATGTGTATGATAAGATCAATGGCGTATGGAATCTGTCCAGCGACCAGGGGAATTTAGGAACTTTTTTTATTACCAATGTGAGAATTGTGTGGCATGCCAATATGAATGACAGTTTTAATGTCAGCATACCATATCTGCAAATT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGAGAATTGTGTGGCATGC
59.73
CCAATAGAAAGATTGACTCTGGGC
59.42
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCAATGTGAGAATTGTGTGGC
59.7
AGAAAGATTGACTCTGGGCA
56.44
140
PCR template sequence:
CCAGGGGAATTTAGGAACTTTTTTTATTACCAATGTGAGAATTGTGTGGCATGCCAATATGAATGACAGTTTTAATGTCAGCATACCATATCTGCAAATTGTAGACTCTTAGTCACAAACTTAAGGATCATCTGGCATTCCTTGGCATTGCCCAGAGTCAATCTTTCTATTGGTTACAACTGCATATTGAATATCACAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-326-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATATCTGCAAATTGTAGACTCTTAGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBBS5_348_ORF_1
1.0382
0.040622535
0.176690388
225
ORF sequence:
ATGTACCGTGACTTTAAGTTGAGAAGTGCAGTCATTCAGAACAAGCAGCTGAGGTTACTACCACAGGAGCATGTGTATGATAAGATCAATGGCGTATGGAATCTGTCCAGCGACCAGGGGAATTTAGGAACTTTTTTTATTACCAATGTGAGAATTGTGTGGCATGCCAATATGAATGACAGTTTTAATGTCAGCATACCATATCTGCAAATTGTAGACTCTTAG
Peptide sequence:
MYRDFKLRSAVIQNKQLRLLPQEHVYDKINGVWNLSSDQGNLGTFFITNVRIVWHANMNDSFNVSIPYLQIVDS
IRESfinder result
IRESfinder score
0.74969553
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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