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mmu_circBaz2b_993
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz2b_993
mm39_Chr. Position ID:
chr2|59748782|59756504|-
Gene name:
Baz2b
Transcript:
ENSMUST00000112550.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
24,23,22,21,20,19
Exon/intron offsets:
0,1856,3704,5102,6222,7581
Genomic length:
7722
Exon/intron sizes in nt:
6,32,44,75,21,52,55,140
Flanking exon/introns:
chr2:59747842-59748782|chr2:59756504-59762448
Splice length:
993
Other database IDs:
circAtlas: mmu-Baz2b_0117
Human Ortholog IDs:
Mature spliced Sequence:
GAAAAAGAACGATTGAAGCAAGAGAAACGTGATGAGAAAAGATTGAATAAGGAACGCAAACTAGAGCAGCGGAGACTGGAGTTAGAAATGGCGAAGGAGCTAAAGAAACCTAAGGAAGACATGTGCTTAGCAGACCAAAAGCCCTTGCCAGAGTGGCCTCGCATCCCGGGACTTGTGCTCTCTGGAACCACATTTTCAGACTGTCTCATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCTTTGATGTGAATATTGATGTTCCAAACCTCAGTGTTCTTCAAGAGGGATTGCTAAATATAGGGGACAGCATGGGTGAAGTACAAGACTTGCTTGTGAGGCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGGCAAAAACAGCTCTGGGAGAACATTTGCTGAACGTTGGTGTGAATCGAGACAATGTGTCCGAGGTTTTGCAGATTTTCATGGAGGCCCACTGTGGGCAGACTGAGCTGACTGAGAGCCTGAAGACTAAAGCTTTCCAGGCTCACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTCGTTAACGAGCTGGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTTGAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAAGCTTAGGATAATTCATGCTAAGAAAACAGGCAAGAGAGACGCCTCAGGGGGCATTGACCTCGGGGAAGAACAACACCCCTTGGGCACGCCCACGCCAGGACGCAAGCGGAGAAGGAAGGGAGGGGACAGTGATTATGACGATGATGACGATGATGACAGCGACGACCAAGCTGATGAGGATGAGGAAGATGAGGAAGATAAAGATGACAAAAAAGGAAAGAAGACCGATATCTGTGAAGATGAGGATGAAGGTGACCAAACAGCCAGCGTTGAGGAGCTAGAAAAACAGATTGAAAAACTGAGCAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGGTGACCAAACAGCCA
59.74
CTAACTCCAGTCTCCGCTGC
60.18
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGGTGACCAAACAGCCA
59.74
TAACTCCAGTCTCCGCTGCT
60.61
145
PCR template sequence:
CAAAAAAGGAAAGAAGACCGATATCTGTGAAGATGAGGATGAAGGTGACCAAACAGCCAGCGTTGAGGAGCTAGAAAAACAGATTGAAAAACTGAGCAAAGAAAAAGAACGATTGAAGCAAGAGAAACGTGATGAGAAAAGATTGAATAAGGAACGCAAACTAGAGCAGCGGAGACTGGAGTTAGAAATGGCGAAGGAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-122-5p(1), mmu-miR-146a-5p(1), mmu-miR-216a-5p(1), mmu-miR-125b-5p(1), mmu-miR-351-5p(1), mmu-miR-125a-5p(1), mmu-miR-676-3p(1), mmu-miR-207(1), mmu-miR-484(1), mmu-miR-452-3p(1), mmu-miR-3061-5p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAACTGAGCAAAGAAAAAGAACGAT
siRNA sets
ATCGTTCTTTTTCTTTGCT,TTCTTTTTCTTTGCTCAGT,GTTCTTTTTCTTTGCTCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ2B_993_ORF_1
1.1356
0.333817195
0.999993148
1899
ORF sequence:
ATGGCGAAGGAGCTAAAGAAACCTAAGGAAGACATGTGCTTAGCAGACCAAAAGCCCTTGCCAGAGTGGCCTCGCATCCCGGGACTTGTGCTCTCTGGAACCACATTTTCAGACTGTCTCATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCTTTGATGTGAATATTGATGTTCCAAACCTCAGTGTTCTTCAAGAGGGATTGCTAAATATAGGGGACAGCATGGGTGAAGTACAAGACTTGCTTGTGAGGCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGGCAAAAACAGCTCTGGGAGAACATTTGCTGAACGTTGGTGTGAATCGAGACAATGTGTCCGAGGTTTTGCAGATTTTCATGGAGGCCCACTGTGGGCAGACTGAGCTGACTGAGAGCCTGAAGACTAAAGCTTTCCAGGCTCACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTCGTTAACGAGCTGGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTTGAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAAGCTTAGGATAATTCATGCTAAGAAAACAGGCAAGAGAGACGCCTCAGGGGGCATTGACCTCGGGGAAGAACAACACCCCTTGGGCACGCCCACGCCAGGACGCAAGCGGAGAAGGAAGGGAGGGGACAGTGATTATGACGATGATGACGATGATGACAGCGACGACCAAGCTGATGAGGATGAGGAAGATGAGGAAGATAAAGATGACAAAAAAGGAAAGAAGACCGATATCTGTGAAGATGAGGATGAAGGTGACCAAACAGCCAGCGTTGAGGAGCTAGAAAAACAGATTGAAAAACTGAGCAAAGAAAAAGAACGATTGAAGCAAGAGAAACGTGATGAGAAAAGATTGAATAAGGAACGCAAACTAGAGCAGCGGAGACTGGAGTTAGAAATGGCGAAGGAGCTAAAGAAACCTAAGGAAGACATGTGCTTAGCAGACCAAAAGCCCTTGCCAGAGTGGCCTCGCATCCCGGGACTTGTGCTCTCTGGAACCACATTTTCAGACTGTCTCATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCTTTGATGTGAATATTGATGTTCCAAACCTCAGTGTTCTTCAAGAGGGATTGCTAAATATAGGGGACAGCATGGGTGAAGTACAAGACTTGCTTGTGAGGCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGGCAAAAACAGCTCTGGGAGAACATTTGCTGAACGTTGGTGTGAATCGAGACAATGTGTCCGAGGTTTTGCAGATTTTCATGGAGGCCCACTGTGGGCAGACTGAGCTGACTGAGAGCCTGAAGACTAAAGCTTTCCAGGCTCACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTCGTTAACGAGCTGGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTTGAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAAGCTTAGGATAATTCATGCTAAGAAAACAGGCAAGAGAGACGCCTCAGGGGGCATTGACCTCGGGGAAGAACAACACCCCTTGGGCACGCCCACGCCAGGACGCAAGCGGAGAAGGAAGGGAGGGGACAGTGATTATGACGATGATGACGATGATGACAGCGACGACCAAGCTGATGAGGATGAGGAAGATGAGGAAGATAAAGATGACAAAAAAGGAAAGAAGACCGATATCTGTGAAGATGAGGATGAAGGTGACCAAACAGCCAGCGTTGAGGAGCTAGAAAAACAGATTGAAAAACTGAGCAAA
Peptide sequence:
MAKELKKPKEDMCLADQKPLPEWPRIPGLVLSGTTFSDCLMVVQFLRNFGKVLGFDVNIDVPNLSVLQEGLLNIGDSMGEVQDLLVRLLSAAVCDPGLITGYKAKTALGEHLLNVGVNRDNVSEVLQIFMEAHCGQTELTESLKTKAFQAHTPAQKASILAFLVNELACSKSVVSEIDKNIEYMSNLRRDKWMVEGKLRKLRIIHAKKTGKRDASGGIDLGEEQHPLGTPTPGRKRRRKGGDSDYDDDDDDDSDDQADEDEEDEEDKDDKKGKKTDICEDEDEGDQTASVEELEKQIEKLSKEKERLKQEKRDEKRLNKERKLEQRRLELEMAKELKKPKEDMCLADQKPLPEWPRIPGLVLSGTTFSDCLMVVQFLRNFGKVLGFDVNIDVPNLSVLQEGLLNIGDSMGEVQDLLVRLLSAAVCDPGLITGYKAKTALGEHLLNVGVNRDNVSEVLQIFMEAHCGQTELTESLKTKAFQAHTPAQKASILAFLVNELACSKSVVSEIDKNIEYMSNLRRDKWMVEGKLRKLRIIHAKKTGKRDASGGIDLGEEQHPLGTPTPGRKRRRKGGDSDYDDDDDDDSDDQADEDEEDEEDKDDKKGKKTDICEDEDEGDQTASVEELEKQIEKLSK
IRESfinder result
IRESfinder score
0.318656513
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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