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mmu_circBaz2b_656
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz2b_656
mm39_Chr. Position ID:
chr2|59743883|59744539|-
Gene name:
Baz2b
Transcript:
ENSMUST00000112550.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
26
Exon/intron offsets:
0
Genomic length:
656
Exon/intron sizes in nt:
656
Flanking exon/introns:
chr2:59743349-59743883|chr2:59744539-59747697
Splice length:
656
Other database IDs:
CIRCpedia: MMU_CIRCpedia_37620
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_146220
Human Ortholog IDs:
Mature spliced Sequence:
GCCTAGAAGAAATTGCAAAAGAAAAAGAAAAGCTTAAAAAGGCAGAGAGTCTCCAGATCAAAGAAGAAGTATTTGAGACTTCTGCGGAGACATTAAACTGTTCCATTAGAGATCACTGCGAGCAGAAAGACGACCCAAAAGAGAAAGATAACACAAATCTATTTCTTCAGAAACCAGGCTCTTTCTCCAAGTTAAGCAAGCTTTTAGAGGTAGCTAAAATGCCTCCTGAGTCAGATGTTATGACCCCCCCAAAAGTGAATGTTAGTACAAACGGGGGCCCACTGTCTCATCAGAACAGCGGGAAGCATCCACTGGGGAGCGTTCCCTCAGCAACAACAGCACAAAGCCCCGTGGGAAAGACAGACGCCAGTCTCTTTAGCTCAGGCTCCGGCAGTTGTGGGAAGTTCTACAGTCCTCTCCCCAATGACCAGCTGTTAAAAACACTGACTGAAAAGAACAGACAGTGGTTTAGCCTTTTGCCAAAAACACCTTGCGATGACACGTCCCTGACCCACGCTGACCTGTCCACCACTCTGGTGACTCCTCAGTCTCAGCCACCATCTAAGTCACCTTCGCCGGCCCCAGCTGCACTGCTCGGACCCTCTTCTGTTCAGAGCCCCCCTGGACTGAACCCGTTTGCTCTGTCGCCTCTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCGGACCCTCTTCTGTTC
60.11
TGTCTCCGCAGAAGTCTCAA
58.67
156
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTTCTGTTCAGAGCCCCC
59.75
TGTCTCCGCAGAAGTCTCAA
58.67
147
PCR template sequence:
ACCATCTAAGTCACCTTCGCCGGCCCCAGCTGCACTGCTCGGACCCTCTTCTGTTCAGAGCCCCCCTGGACTGAACCCGTTTGCTCTGTCGCCTCTTCAGGCCTAGAAGAAATTGCAAAAGAAAAAGAAAAGCTTAAAAAGGCAGAGAGTCTCCAGATCAAAGAAGAAGTATTTGAGACTTCTGCGGAGACATTAAACTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-181b-5p(1), mmu-miR-182-5p(1), mmu-miR-690(1), mmu-miR-29b-2-5p(1), mmu-miR-188-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCGCCTCTTCAGGCCTAGAAGAAAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ2B_656_ORF_1
0.8931
0.129555045
0.53016446
444
ORF sequence:
ATGCCTCCTGAGTCAGATGTTATGACCCCCCCAAAAGTGAATGTTAGTACAAACGGGGGCCCACTGTCTCATCAGAACAGCGGGAAGCATCCACTGGGGAGCGTTCCCTCAGCAACAACAGCACAAAGCCCCGTGGGAAAGACAGACGCCAGTCTCTTTAGCTCAGGCTCCGGCAGTTGTGGGAAGTTCTACAGTCCTCTCCCCAATGACCAGCTGTTAAAAACACTGACTGAAAAGAACAGACAGTGGTTTAGCCTTTTGCCAAAAACACCTTGCGATGACACGTCCCTGACCCACGCTGACCTGTCCACCACTCTGGTGACTCCTCAGTCTCAGCCACCATCTAAGTCACCTTCGCCGGCCCCAGCTGCACTGCTCGGACCCTCTTCTGTTCAGAGCCCCCCTGGACTGAACCCGTTTGCTCTGTCGCCTCTTCAGGCCTAG
Peptide sequence:
MPPESDVMTPPKVNVSTNGGPLSHQNSGKHPLGSVPSATTAQSPVGKTDASLFSSGSGSCGKFYSPLPNDQLLKTLTEKNRQWFSLLPKTPCDDTSLTHADLSTTLVTPQSQPPSKSPSPAPAALLGPSSVQSPPGLNPFALSPLQA
IRESfinder result
IRESfinder score
0.593917027
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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