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mmu_circBaz2b_498
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz2b_498
mm39_Chr. Position ID:
chr2|59765558|59778641|-
Gene name:
Baz2b
Transcript:
ENSMUST00000112550.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
16,15,14,13,12
Exon/intron offsets:
0,1471,7547,7723,12872
Genomic length:
13083
Exon/intron sizes in nt:
66,66,62,93,211
Flanking exon/introns:
chr2:59764059-59765558|chr2:59778641-59787973
Splice length:
498
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
GGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGAAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCAGCAGATTCTCGAGGCT
59.25
CGTCCATTGCCCTGATACGA
59.9
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTCAGCAGATTCTCGAGG
59.25
CGTCCATTGCCCTGATACGA
59.9
146
PCR template sequence:
GGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1), mmu-miR-370-3p(1), mmu-miR-541-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
GAAACGAACCAAGGGAATGCAGTGGT
siRNA sets
CTGCATTCCCTTGGTTCGT, CATTCCCTTGGTTCGTTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ2B_498_ORF_1
0.9965
0.213770962
0.990306581
993
ORF sequence:
ATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGAAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGAAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAG
Peptide sequence:
MQWCLLKEEDVIPRIRAMDGRRGRPPNPDRPRAREESRMKRRKGRPPNVGSAEFLDNTDAKLLRKLQAQEIARQAAQIKLLRKLQKQEQARVAKEAKKQQAIMAAEEKRKQKEQMKIIKQQEKIKRIQQIRMEKELRAQQILEAKKKKKEEAANAKLLEAEKRTKGMQWCLLKEEDVIPRIRAMDGRRGRPPNPDRPRAREESRMKRRKGRPPNVGSAEFLDNTDAKLLRKLQAQEIARQAAQIKLLRKLQKQEQARVAKEAKKQQAIMAAEEKRKQKEQMKIIKQQEKIKRIQQIRMEKELRAQQILEAKKKKKEEAANAKLLEAEKRTK
IRESfinder result
IRESfinder score
0.193646416
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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