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mmu_circBaz2b_381
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz2b_381
mm39_Chr. Position ID:
chr2|59778430|59788836|-
Gene name:
Baz2b
Transcript:
ENSMUST00000112550.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
12,11,10
Exon/intron offsets:
0,9543,10345
Genomic length:
10406
Exon/intron sizes in nt:
211,109,61
Flanking exon/introns:
chr2:59773374-59778430|chr2:59788836-59790164
Splice length:
381
Other database IDs:
circBase: mmu_circ_0010055, CIRCpedia: MMU_CIRCpedia_24730, circAtlas: mmu-Baz2b_0046
circAtlas: hsa-BAZ2B_0106
Human Ortholog IDs:
Mature spliced Sequence:
GTACATCCAAACGAAGAAGAGTGGCAGACGATCAGGAGCTGCGCATCCCTCTGGACTATGGCTGGCAGAGGGAGACAAGGGTGAGAAACTTTGGTGGGCGCCTTCCAGGAGAAGTTGCATATTATGCCCCGTGTGGGAAGAAGCTGAGGCAGTGCCCTGACATGGTAAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAATGTTGGCAGTGCAGAA
59.32
CGCCCACCAAAGTTTCTCAC
59.69
169
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTTGGCAGTGCAGAATTCC
59.04
CGCCCACCAAAGTTTCTCAC
59.69
165
PCR template sequence:
TCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGGTACATCCAAACGAAGAAGAGTGGCAGACGATCAGGAGCTGCGCATCCCTCTGGACTATGGCTGGCAGAGGGAGACAAGGGTGAGAAACTTTGGTGGGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(2), mmu-miR-204-5p(1), mmu-miR-541-5p(1), mmu-miR-324-3p(1), mmu-miR-207(1), mmu-miR-188-3p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
CTGCAGGCTCAAGGTACATCCAAACG
siRNA sets
TTTGGATGTACCTTGAGCC, TTGGATGTACCTTGAGCCT, GTTTGGATGTACCTTGAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ2B_381_ORF_1
0.7913
0.171516004
0.775849291
600
ORF sequence:
ATGGTAAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGGTACATCCAAACGAAGAAGAGTGGCAGACGATCAGGAGCTGCGCATCCCTCTGGACTATGGCTGGCAGAGGGAGACAAGGGTGAGAAACTTTGGTGGGCGCCTTCCAGGAGAAGTTGCATATTATGCCCCGTGTGGGAAGAAGCTGAGGCAGTGCCCTGACATGGTAAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAA
Peptide sequence:
MVKGMQWCLLKEEDVIPRIRAMDGRRGRPPNPDRPRAREESRMKRRKGRPPNVGSAEFLDNTDAKLLRKLQAQGTSKRRRVADDQELRIPLDYGWQRETRVRNFGGRLPGEVAYYAPCGKKLRQCPDMVKGMQWCLLKEEDVIPRIRAMDGRRGRPPNPDRPRAREESRMKRRKGRPPNVGSAEFLDNTDAKLLRKLQAQ
IRESfinder result
IRESfinder score
0.215671071
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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