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mmu_circBaz2b_359
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz2b_359
mm39_Chr. Position ID:
chr2|59763755|59773374|-
Gene name:
Baz2b
Transcript:
ENSMUST00000153136.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,259,1803,3274,9350,9526
Genomic length:
9619
Exon/intron sizes in nt:
27,45,66,66,62,93
Flanking exon/introns:
chr2:59762514-59763755|chr2:59773374-59778430
Splice length:
359
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAGGAGAAAGAACTGAGAAGACAGCAGGCTGTCCTTCTGAAACACCAGGAGTTGGAGAGGCATAGACTAGATATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATTGGAGGCCGAGAAACGAA
59.68
TAGCTTCTTTGGCAACCCGT
59.89
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATTGGAGGCCGAGAAACGAA
59.68
GTGCCTGTTCCTGCTTCTGA
60.25
160
PCR template sequence:
ATTATTGGAGGCCGAGAAACGAACCAAGGAGAAAGAACTGAGAAGACAGCAGGCTGTCCTTCTGAAACACCAGGAGTTGGAGAGGCATAGACTAGATATGAAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(1), mmu-miR-370-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TAGACTAGATATGAAATAGCCAGACA
siRNA sets
TGTCTGGCTATTTCATATC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ2B_359_ORF_1
1.1389
0.306808847
0.509803489
267
ORF sequence:
ATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAGGAGAAAGAACTGAGAAGACAGCAGGCTGTCCTTCTGAAACACCAGGAGTTGGAGAGGCATAGACTAGATATGAAATAG
Peptide sequence:
MAAEEKRKQKEQMKIIKQQEKIKRIQQIRMEKELRAQQILEAKKKKKEEAANAKLLEAEKRTKEKELRRQQAVLLKHQELERHRLDMK
IRESfinder result
IRESfinder score
0.209750768
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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