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mmu_circBaz2b_3151
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz2b_3151
mm39_Chr. Position ID:
chr2|59752486|59807896|-
Gene name:
Baz2b
Transcript:
ENSMUST00000112550.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
18
Exon/intron indexes:
22,21,20,19,18,17,16,15,14,13,12,11,10,9,8,7,6,5
Exon/intron offsets:
0,1398,2518,3877,9962,11528,13072,14543,20619,20795,25944,35487,36289,37678,39878,46484,47075,55216
Genomic length:
55410
Exon/intron sizes in nt:
7,52,15,25,51,41,66,45,00,00,00,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:59750882-59752486|chr2:59807896-59808886
Splice length:
3151
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTATAAATGGGTCGGTTAATGGAACCAGTGCAGCATCAGTACTTGGTGTCAATGCATCTGTAGTAGCCACTCCTGCTTCAAGTTCCATGGGACAGAACCAAAGCACAAGCTCAGGTGGAGGAACCCTAAAATGTCATCAGGAACAAAACAAAAGCCAGCCTGTGGACGCCAGAGCCGACAGAATCAAAGATAAGAAACCAAGGAAGAAGGCTATGGAAAGTTCTAGCAACAGTGATAGTGACTCAGGCACATCGTCAGACACCTCGAGTGAAGGCATTAGCAGCAGTGACTCAGACGATCTGGAAGAGGAAGAGGAAGAAGATCAAAGCGTGGAGGAGAGTGAAGATGACGATTCTGACTCAGAGACTGAAGCACAACACAAAAGTAACAACCAGGTGCTATTACATGGTATTTCAGACCCAAAAACAGATGGACAGAAAGCAACTGAAAAAGCCCAGGAAAGAAGAACACACCAGCCATTACCTCCTGTGTCTGAGTCCCAGACGCACCCACCATTCCAGTCCCAGCAGAAGCAGCCTCAGGTTCTCTCACAGCAGCTTCCGTTTATTTTCCAAAGCTCTCAGGCAAAGGAGGAGTCTGTGACCAAACACACCAGTGTAATACAGTCGACGGGACTGGTGTCCAATGTGAAACCTTTGTCTTTGGTAAATCAAGCCAAAAAGGAAACTTACAGGAAACTCGTAGTTCCTTCTCCTGATGTACTTAAAGCAGGGAATAAAAATACCTCTGAAGAGTCTAGTTCTTTGACCAGTGAATTGCGATCCAAACGGGAACAATATAAGCAGACATTCCCATCACAAGGAAAGAAACAGGAGATGGGGAAGAGCCTGAAGAAGGTTATTGCAGCTTTGTCAAATACTAAAGCAACCTCTAGCTCACCAGCACATCCAAAACTACCACTAGATAACAACCACCCTAATCCATTTTTGACAAATGCACTTTTAGGGAATCACCAACCAAATGGAGTCATTCAAAGCGTCATTCAAGAAGCTCCGTTAGCACTAACTACCAAAACGAAAATGCAGAGTAAGATTAACGAAAATGTCTCAAGTAGCACCCCGTTCTCCTCACCTGTCAATCTGAGCACGAGTGGGAGGAGAGCCCCTGGCAGTCAGACTCCAGCGCTGCCCTCTGCCTCGCCCATCCTGCACAGCAGTGGGAAGGAAAAGCGGGTCAGCAATGACGCAACCCCGCTCAAAGCGCACCATCACCCCCATCCGGCGGCAGCGGCCGCGGCCTTGGTGGAACAGTTTAGAGGGACAGATTCAGACGTTCCCAGTAGTAAAGACTCTGAGGACTCAAATGAGGATGAGGAGGAAGATGATGAGGAAGAAGATGAAGAGGATGATGAAGATGATGAGTCTGATGACAGTCAATCAGAATCAGATAGTAATTCACAATCAGATTCAGAAGGCTCAGAAGACGACGAGGAGAAAGACCAGGAGGAATCAGATAGTGACACTGAAGGCGAGAAACCTGCCGTGAACCTGACCCAGACAAGCTCCTCTGCCAAAAGCCCTCCCTCCAGTCTCACAGCGCACTCAGCACCTCACCTCCACATAGGAAGCCCCCCAGGCTCTGCTCCTGCTGCCCTGTGCTCTGAGTCCCAGCCTCCTGCTTTTCTTGGCACTTCTTCTTCCACACTCACTTCAACTCCACACTCTGGTACATCCAAACGAAGAAGAGTGGCAGACGATCAGGAGCTGCGCATCCCTCTGGACTATGGCTGGCAGAGGGAGACAAGGGTGAGAAACTTTGGTGGGCGCCTTCCAGGAGAAGTTGCATATTATGCCCCGTGTGGGAAGAAGCTGAGGCAGTGCCCTGACATGGTAAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGAAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAGGAGAAAGAACTGAGAAGACAGCAGGCTGTCCTTCTGAAACACCAGGAACGGGAGCGAAGGCGGCAGCATGTGATGCTTATGAAAGCTATGGAAGCTCGTAAAAAAGCAGAAGAAAAAGAACGATTGAAGCAAGAGAAACGTGATGAGAAAAGATTGAATAAGGAACGCAAACTAGAGCAGCGGAGACTGGAGTTAGAAATGGCGAAGGAGCTAAAGAAACCTAAGGAAGACATGTGCTTAGCAGACCAAAAGCCCTTGCCAGAGTGGCCTCGCATCCCGGGACTTGTGCTCTCTGGAACCACATTTTCAGACTGTCTCATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCTTTGATGTGAATATTGATGTTCCAAACCTCAGTGTTCTTCAAGAGGGATTGCTAAATATAGGGGACAGCATGGGTGAAGTACAAGACTTGCTTGTGAGGCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGGCAAAAACAGCTCTGGGAGAACATTTGCTGAACGTTGGTGTGAATCGAGACAATGTGTCCGAGGTTTTGCAGATTTTCATGGAGGCCCACTGTGGGCAGACTGAGCTGACTGAGAGCCTGAAGACTAAAGCTTTCCAGGCTCACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTCGTTAACGAGCTGGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTTGAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAAGAGTGTGGTGAGTGAGA
59.93
AACTTGAAGCAGGAGTGGCT
59.52
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAAGAGTGTGGTGAGTGAG
59.93
AACTTGAAGCAGGAGTGGCT
59.52
176
PCR template sequence:
TGGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTTGAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAAGTATAAATGGGTCGGTTAATGGAACCAGTGCAGCATCAGTACTTGGTGTCAATGCATCTGTAGTAGCCACTCCTGCTTCAAGTTCCATGGGACAGAACCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-1199-5p(3), mmu-miR-574-5p(3), mmu-miR-211-5p(2), mmu-miR-298-5p(2), mmu-miR-136-5p(2), mmu-miR-370-3p(2), mmu-miR-484(2), mmu-miR-9-5p(1), mmu-miR-93-5p(1), mmu-let-7b-5p(1), mmu-miR-122-5p(1), mmu-miR-204-5p(1), mmu-miR-146a-5p(1), mmu-miR-216a-5p(1), mmu-miR-709(1), mmu-miR-214-3p(1), mmu-miR-541-5p(1), mmu-miR-324-3p(1), mmu-miR-342-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-532-5p(1), mmu-miR-452-3p(1), mmu-miR-188-3p(1), mmu-miR-697(1), mmu-miR-1904(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTAAACTCCGCAAGTATAAATGGGTC
siRNA sets
TTATACTTGCGGAGTTTAC, TTTATACTTGCGGAGTTTA, ATTTATACTTGCGGAGTTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ2B_3151_ORF_1
1.0021
0.22085229
0.999999997
3084
ORF sequence:
ATGGGACAGAACCAAAGCACAAGCTCAGGTGGAGGAACCCTAAAATGTCATCAGGAACAAAACAAAAGCCAGCCTGTGGACGCCAGAGCCGACAGAATCAAAGATAAGAAACCAAGGAAGAAGGCTATGGAAAGTTCTAGCAACAGTGATAGTGACTCAGGCACATCGTCAGACACCTCGAGTGAAGGCATTAGCAGCAGTGACTCAGACGATCTGGAAGAGGAAGAGGAAGAAGATCAAAGCGTGGAGGAGAGTGAAGATGACGATTCTGACTCAGAGACTGAAGCACAACACAAAAGTAACAACCAGGTGCTATTACATGGTATTTCAGACCCAAAAACAGATGGACAGAAAGCAACTGAAAAAGCCCAGGAAAGAAGAACACACCAGCCATTACCTCCTGTGTCTGAGTCCCAGACGCACCCACCATTCCAGTCCCAGCAGAAGCAGCCTCAGGTTCTCTCACAGCAGCTTCCGTTTATTTTCCAAAGCTCTCAGGCAAAGGAGGAGTCTGTGACCAAACACACCAGTGTAATACAGTCGACGGGACTGGTGTCCAATGTGAAACCTTTGTCTTTGGTAAATCAAGCCAAAAAGGAAACTTACAGGAAACTCGTAGTTCCTTCTCCTGATGTACTTAAAGCAGGGAATAAAAATACCTCTGAAGAGTCTAGTTCTTTGACCAGTGAATTGCGATCCAAACGGGAACAATATAAGCAGACATTCCCATCACAAGGAAAGAAACAGGAGATGGGGAAGAGCCTGAAGAAGGTTATTGCAGCTTTGTCAAATACTAAAGCAACCTCTAGCTCACCAGCACATCCAAAACTACCACTAGATAACAACCACCCTAATCCATTTTTGACAAATGCACTTTTAGGGAATCACCAACCAAATGGAGTCATTCAAAGCGTCATTCAAGAAGCTCCGTTAGCACTAACTACCAAAACGAAAATGCAGAGTAAGATTAACGAAAATGTCTCAAGTAGCACCCCGTTCTCCTCACCTGTCAATCTGAGCACGAGTGGGAGGAGAGCCCCTGGCAGTCAGACTCCAGCGCTGCCCTCTGCCTCGCCCATCCTGCACAGCAGTGGGAAGGAAAAGCGGGTCAGCAATGACGCAACCCCGCTCAAAGCGCACCATCACCCCCATCCGGCGGCAGCGGCCGCGGCCTTGGTGGAACAGTTTAGAGGGACAGATTCAGACGTTCCCAGTAGTAAAGACTCTGAGGACTCAAATGAGGATGAGGAGGAAGATGATGAGGAAGAAGATGAAGAGGATGATGAAGATGATGAGTCTGATGACAGTCAATCAGAATCAGATAGTAATTCACAATCAGATTCAGAAGGCTCAGAAGACGACGAGGAGAAAGACCAGGAGGAATCAGATAGTGACACTGAAGGCGAGAAACCTGCCGTGAACCTGACCCAGACAAGCTCCTCTGCCAAAAGCCCTCCCTCCAGTCTCACAGCGCACTCAGCACCTCACCTCCACATAGGAAGCCCCCCAGGCTCTGCTCCTGCTGCCCTGTGCTCTGAGTCCCAGCCTCCTGCTTTTCTTGGCACTTCTTCTTCCACACTCACTTCAACTCCACACTCTGGTACATCCAAACGAAGAAGAGTGGCAGACGATCAGGAGCTGCGCATCCCTCTGGACTATGGCTGGCAGAGGGAGACAAGGGTGAGAAACTTTGGTGGGCGCCTTCCAGGAGAAGTTGCATATTATGCCCCGTGTGGGAAGAAGCTGAGGCAGTGCCCTGACATGGTAAAGGGAATGCAGTGGTGTCTTTTGAAAGAAGAGGATGTCATTCCTCGTATCAGGGCAATGGACGGCCGAAGAGGAAGACCACCCAATCCAGATAGACCCAGAGCAAGAGAGGAATCCAGGATGAAACGCCGGAAGGGTCGCCCTCCCAATGTTGGCAGTGCAGAATTCCTAGATAACACAGATGCAAAATTACTAAGGAAACTGCAGGCTCAAGAAATAGCCAGACAAGCAGCACAAATAAAACTTTTGAGAAAACTTCAGAAGCAGGAACAGGCACGGGTTGCCAAAGAAGCTAAAAAACAGCAAGCGATAATGGCTGCGGAAGAGAAGCGCAAACAGAAAGAACAGATGAAGATTATAAAGCAGCAGGAAAAAATTAAGAGAATACAGCAAATCAGAATGGAGAAAGAACTGAGAGCTCAGCAGATTCTCGAGGCTAAAAAGAAAAAGAAGGAAGAAGCGGCAAATGCCAAATTATTGGAGGCCGAGAAACGAACCAAGGAGAAAGAACTGAGAAGACAGCAGGCTGTCCTTCTGAAACACCAGGAACGGGAGCGAAGGCGGCAGCATGTGATGCTTATGAAAGCTATGGAAGCTCGTAAAAAAGCAGAAGAAAAAGAACGATTGAAGCAAGAGAAACGTGATGAGAAAAGATTGAATAAGGAACGCAAACTAGAGCAGCGGAGACTGGAGTTAGAAATGGCGAAGGAGCTAAAGAAACCTAAGGAAGACATGTGCTTAGCAGACCAAAAGCCCTTGCCAGAGTGGCCTCGCATCCCGGGACTTGTGCTCTCTGGAACCACATTTTCAGACTGTCTCATGGTGGTGCAGTTCTTACGAAACTTTGGTAAAGTTTTGGGCTTTGATGTGAATATTGATGTTCCAAACCTCAGTGTTCTTCAAGAGGGATTGCTAAATATAGGGGACAGCATGGGTGAAGTACAAGACTTGCTTGTGAGGCTCCTCTCAGCTGCTGTATGTGATCCAGGTCTAATTACAGGATACAAGGCAAAAACAGCTCTGGGAGAACATTTGCTGAACGTTGGTGTGAATCGAGACAATGTGTCCGAGGTTTTGCAGATTTTCATGGAGGCCCACTGTGGGCAGACTGAGCTGACTGAGAGCCTGAAGACTAAAGCTTTCCAGGCTCACACTCCAGCACAGAAAGCGTCCATCCTGGCCTTCCTCGTTAACGAGCTGGCATGCAGCAAGAGTGTGGTGAGTGAGATTGACAAGAACATTGAATACATGTCTAACTTGAGAAGAGATAAATGGATGGTGGAAGGTAAACTCCGCAAGTATAAATGGGTCGGTTAA
Peptide sequence:
MGQNQSTSSGGGTLKCHQEQNKSQPVDARADRIKDKKPRKKAMESSSNSDSDSGTSSDTSSEGISSSDSDDLEEEEEEDQSVEESEDDDSDSETEAQHKSNNQVLLHGISDPKTDGQKATEKAQERRTHQPLPPVSESQTHPPFQSQQKQPQVLSQQLPFIFQSSQAKEESVTKHTSVIQSTGLVSNVKPLSLVNQAKKETYRKLVVPSPDVLKAGNKNTSEESSSLTSELRSKREQYKQTFPSQGKKQEMGKSLKKVIAALSNTKATSSSPAHPKLPLDNNHPNPFLTNALLGNHQPNGVIQSVIQEAPLALTTKTKMQSKINENVSSSTPFSSPVNLSTSGRRAPGSQTPALPSASPILHSSGKEKRVSNDATPLKAHHHPHPAAAAAALVEQFRGTDSDVPSSKDSEDSNEDEEEDDEEEDEEDDEDDESDDSQSESDSNSQSDSEGSEDDEEKDQEESDSDTEGEKPAVNLTQTSSSAKSPPSSLTAHSAPHLHIGSPPGSAPAALCSESQPPAFLGTSSSTLTSTPHSGTSKRRRVADDQELRIPLDYGWQRETRVRNFGGRLPGEVAYYAPCGKKLRQCPDMVKGMQWCLLKEEDVIPRIRAMDGRRGRPPNPDRPRAREESRMKRRKGRPPNVGSAEFLDNTDAKLLRKLQAQEIARQAAQIKLLRKLQKQEQARVAKEAKKQQAIMAAEEKRKQKEQMKIIKQQEKIKRIQQIRMEKELRAQQILEAKKKKKEEAANAKLLEAEKRTKEKELRRQQAVLLKHQERERRRQHVMLMKAMEARKKAEEKERLKQEKRDEKRLNKERKLEQRRLELEMAKELKKPKEDMCLADQKPLPEWPRIPGLVLSGTTFSDCLMVVQFLRNFGKVLGFDVNIDVPNLSVLQEGLLNIGDSMGEVQDLLVRLLSAAVCDPGLITGYKAKTALGEHLLNVGVNRDNVSEVLQIFMEAHCGQTELTESLKTKAFQAHTPAQKASILAFLVNELACSKSVVSEIDKNIEYMSNLRRDKWMVEGKLRKYKWVG
IRESfinder result
IRESfinder score
0.432183484
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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