top of page
mmu_circBaz1b_361
Basic information of circular RNA
PanCircBase ID:
mmu_circBaz1b_361
mm39_Chr. Position ID:
chr5|135259010|135260636|+
Gene name:
Baz1b
Transcript:
ENSMUST00000002825.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
12,13
Exon/intron offsets:
0,1443
Genomic length:
1626
Exon/intron sizes in nt:
1,78,183
Flanking exon/introns:
chr5:135257479-135259010|chr5:135260636-135265128
Splice length:
361
Other database IDs:
CIRCpedia: MMU_CIRCpedia_8251, circAtlas: mmu-Baz1b_0012
circBase: hsa_circ_0002967, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_58715
Human Ortholog IDs:
Mature spliced Sequence:
ATACCAAGAAATTACTCATTCTATTTATCTGGCTCGGAAGCCAAATTTGGGTCTAAAATCTTGTGACGGCAACCAGGAGCTCTTAAACTTCCTGAGGAGTGATCTTATTGAAGTGGCAACGAGGCTGCAGAAAGGAGGACTTGGCTACATGGAGGGAACATCTGAGTTTGAGGCCCGGGTCATTTCCTTAGAGAAACTGAAGGATTTTGGTGAATGCGTCATTGCCCTTCAAGCCAGTGTCATAAAGAAATTTCTGCAAGGATTTATGGCGCCTAAGCAGAAGAAGCGGAAACTCCAAAGTGAAGATTCCACAAAGTCCGAGGAAGTAGATGAAGAGAAAAAGATGGTAGAGGAAGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTAAGCAGAAGAAGCGGA
60.11
TTAAGAGCTCCTGGTTGCCG
60.04
177
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTAAGCAGAAGAAGCGGA
60.11
CCTGGTTGCCGTCACAAGAT
60.61
168
PCR template sequence:
ATTTATGGCGCCTAAGCAGAAGAAGCGGAAACTCCAAAGTGAAGATTCCACAAAGTCCGAGGAAGTAGATGAAGAGAAAAAGATGGTAGAGGAAGCAAAGATACCAAGAAATTACTCATTCTATTTATCTGGCTCGGAAGCCAAATTTGGGTCTAAAATCTTGTGACGGCAACCAGGAGCTCTTAAACTTCCTGAGGAGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-382-3p(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGAGGAAGCAAAGATACCAAGAAATT
siRNA sets
AATTTCTTGGTATCTTTGC, TTGGTATCTTTGCTTCCTC, TTTCTTGGTATCTTTGCTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBAZ1B_361_ORF_1
0.8038
0.223366904
0.301823749
279
ORF sequence:
ATGGAGGGAACATCTGAGTTTGAGGCCCGGGTCATTTCCTTAGAGAAACTGAAGGATTTTGGTGAATGCGTCATTGCCCTTCAAGCCAGTGTCATAAAGAAATTTCTGCAAGGATTTATGGCGCCTAAGCAGAAGAAGCGGAAACTCCAAAGTGAAGATTCCACAAAGTCCGAGGAAGTAGATGAAGAGAAAAAGATGGTAGAGGAAGCAAAGATACCAAGAAATTACTCATTCTATTTATCTGGCTCGGAAGCCAAATTTGGGTCTAAAATCTTGTGA
Peptide sequence:
MEGTSEFEARVISLEKLKDFGECVIALQASVIKKFLQGFMAPKQKKRKLQSEDSTKSEEVDEEKKMVEEAKIPRNYSFYLSGSEAKFGSKIL
IRESfinder result
IRESfinder score
0.505785074
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page