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mmu_circBaz1b_1903

Basic information of circular RNA
PanCircBase ID:

mmu_circBaz1b_1903

mm39_Chr. Position ID:

chr5|135239594|135247148|+

Gene name:

Baz1b

Transcript:

ENSMUST00000002825.6

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

2

Exon/intron indexes:

6,7

Exon/intron offsets:

0,5849

Genomic length:

7554

Exon/intron sizes in nt:

19,81,705

Flanking exon/introns:

chr5:135238018-135239594|chr5:135247148-135250811

Splice length:

1903

Other database IDs:

CIRCpedia: MMU_CIRCpedia_8226, circAtlas: mmu-Baz1b_0009

circAtlas: hsa-BAZ1B_0025, CIRCpedia: HSA_CIRCpedia_117598

Human Ortholog IDs:
Mature spliced Sequence:

ATCATCAGCAATGTGCCAGCAGACAGCTTGATTCGAACGGAGAGACCACCAAATAAGGAGATACTTCGATACTTTATTCGGCATAATGCCTTACGTGCTGGTACTGGTGAGAATGCACCTTGGGTGGTGGAAGATGAATTGGTGAAGAAATATTCCCTGCCTAGCAAGTTCAGTGACTTTTTACTTGACCCATATAAGTATATGACTCTCAATCCCTCTACCAAGAGGAGGAATACTGGATCTCCAGACAGGAAGCCCTCAAAGAAACCTAAGAGAGACAGTTCTTCTCTGAGTTCCCCACTAAACCCTAAGCTGTGGTGTCATGTGCACTTGGAGAAGTCGTTAAATGGCCCACCACTCAAAGTAAAGAACTCAAAGAATTCCAAGTCTCCGGAAGAACATTTGGAAGGGGTGATGAAAATTATGTCCCCCAACAACAACAAGCTACACAGCTTCCACATTCCTAAGAAAGGCCCAGCCGCCAAGAAGCCAGGGAAGCACAGTGACAAACCTCTGAAGGCCAAGGGCAGAGGCAAAGGCATCCTGAATGGACAGAAGTCCACAGGCAACTCCAAATCACCCAGCAAGTGTGTGAAGACTCCTAAGACCAAAATGAAGCAGATGACTTTGTTGGATATGGCCAAAGGGACTCAGAAGATGACCCGCACCCCACGGAGTTCTGGGGGTGTGCCAAGGTCTTCTGGTAAACCTCACAAACACCTGCCTCCTGCAGCTCTGCATCTGATTGCCTATTACAAAGAAAATAAAGACAAAGAGGACAAGAAGAGTGCTCTGTCCTGTGTTATCTCCAAAACAGCACGTCTCCTCTCCAATGAAGATAGAGCCCGTCTTCCAGAAGAGCTCCGGGCTCTTGTCCAGAAACGCTATGAGCTCCTGGAACATAAAAAGAGGTGGGCCTCTATGTCTGAAGAGCAACGGAAAGAATATTTGAAAAAGAAACGACAGGAGCTAAAGGAGAGGTTGCGGGAAAAAGCCAAAGAACGGAGAGAGAGAGAAATGCTGGAGAGGTTAGAAAAGCAGAAGCGATTTGAGGACCAGGAGTTAGGTGGCAGAAACCTCCCAGCATTCAGGCTGGTAGATACCCCTGAGGGGCTCCCCAACACTCTTTTTGGGGATGTGGCCCTGGTGGTGGAGTTCCTGAGCTGTTACTCTGGGTTGCTCTTGCCCGATGCTCAGTACCCTATCACTGCTGTGTCCCTAATGGAAGCCTTGAGTGCAGATAAAGGTGGTTTTTTGTACTTGAACAGAGTGCTGGTCATCCTGCTACAGACCTTACTTCAGGATGAAATTGCAGAAGACTATGGAGAACTGGGAATGAAGCTGTCAGAAATCCCCCTCACCCTGCATTCTGTCTCAGAGCTGGTCCGGCTCTGCTTGCGCAGGTGTGATGTCCAGGAGGACAGCGAGGGCTCAGAAACAGATGACAATAAAGATTCCACACCATTTGAGGATAATGAGGTGCAAGACGAATTTCTAGAGAAGCTGGAGACATCAGAATTCTTTGAGCTGACCTCAGAGGAGAAGCTACGGATATTGACAGCTCTTTGCCACCGTATCCTCATGACATACTCTGTGCAAGACCACATGGAGACAAGACAGCAGGTGTCTGCAGAGTTGTGGAAGGAGCGGCTTGCTGTGCTGAAGGAGGAGAATGATAAGAAGAGAGCAGAGAAGCAAAAGCGGAAGGAGATGGAAGCCAGAAATAAAGAAAATGGCAAAGAGGAGAATGTGCTAGGCAAAGTGGACAGGAAAAAAGAAATTGTGAAGATTGAACAGCAAGTAGAAGTGGAAGCAGATGACATGATCAGTGCTGTCAAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAACAAAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCAGATGACATGATCAGTGCTG

59.71

TTTGGTGGTCTCTCCGTTCG

59.97

144

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

AGAAGGCTGCTTAGCATGCA

60.03

CAGCACGTAAGGCATTATGC

57.61

161

PCR template sequence:

GAAGTGGAAGCAGATGACATGATCAGTGCTGTCAAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAACAAAAGATCATCAGCAATGTGCCAGCAGACAGCTTGATTCGAACGGAGAGACCACCAAATAAGGAGATACTTCGATACTTTATTCGGCATAATGCCTTACGTGCTG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

15

Interacting miRNAs (#binding sites)

mmu-miR-1a-3p(1), mmu-miR-7b-5p(1), mmu-miR-206-3p(1), mmu-let-7b-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-335-5p(1), mmu-miR-18a-5p(1), mmu-miR-431-5p(1), mmu-miR-194-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-3065-5p(1), mmu-miR-1199-5p(1), mmu-miR-1904(1)

siRNAs for circular RNA silencing
Junction sequence

AGAGAAACAAAAGATCATCAGCAATG

siRNA sets

ATTGCTGATGATCTTTTGT,GATGATCTTTTGTTTCTCT,TTGCTGATGATCTTTTGTT

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBAZ1B_1903_ORF_1

1.0973

0.299979576

0.99997561

1758

ORF sequence:

ATGACTCTCAATCCCTCTACCAAGAGGAGGAATACTGGATCTCCAGACAGGAAGCCCTCAAAGAAACCTAAGAGAGACAGTTCTTCTCTGAGTTCCCCACTAAACCCTAAGCTGTGGTGTCATGTGCACTTGGAGAAGTCGTTAAATGGCCCACCACTCAAAGTAAAGAACTCAAAGAATTCCAAGTCTCCGGAAGAACATTTGGAAGGGGTGATGAAAATTATGTCCCCCAACAACAACAAGCTACACAGCTTCCACATTCCTAAGAAAGGCCCAGCCGCCAAGAAGCCAGGGAAGCACAGTGACAAACCTCTGAAGGCCAAGGGCAGAGGCAAAGGCATCCTGAATGGACAGAAGTCCACAGGCAACTCCAAATCACCCAGCAAGTGTGTGAAGACTCCTAAGACCAAAATGAAGCAGATGACTTTGTTGGATATGGCCAAAGGGACTCAGAAGATGACCCGCACCCCACGGAGTTCTGGGGGTGTGCCAAGGTCTTCTGGTAAACCTCACAAACACCTGCCTCCTGCAGCTCTGCATCTGATTGCCTATTACAAAGAAAATAAAGACAAAGAGGACAAGAAGAGTGCTCTGTCCTGTGTTATCTCCAAAACAGCACGTCTCCTCTCCAATGAAGATAGAGCCCGTCTTCCAGAAGAGCTCCGGGCTCTTGTCCAGAAACGCTATGAGCTCCTGGAACATAAAAAGAGGTGGGCCTCTATGTCTGAAGAGCAACGGAAAGAATATTTGAAAAAGAAACGACAGGAGCTAAAGGAGAGGTTGCGGGAAAAAGCCAAAGAACGGAGAGAGAGAGAAATGCTGGAGAGGTTAGAAAAGCAGAAGCGATTTGAGGACCAGGAGTTAGGTGGCAGAAACCTCCCAGCATTCAGGCTGGTAGATACCCCTGAGGGGCTCCCCAACACTCTTTTTGGGGATGTGGCCCTGGTGGTGGAGTTCCTGAGCTGTTACTCTGGGTTGCTCTTGCCCGATGCTCAGTACCCTATCACTGCTGTGTCCCTAATGGAAGCCTTGAGTGCAGATAAAGGTGGTTTTTTGTACTTGAACAGAGTGCTGGTCATCCTGCTACAGACCTTACTTCAGGATGAAATTGCAGAAGACTATGGAGAACTGGGAATGAAGCTGTCAGAAATCCCCCTCACCCTGCATTCTGTCTCAGAGCTGGTCCGGCTCTGCTTGCGCAGGTGTGATGTCCAGGAGGACAGCGAGGGCTCAGAAACAGATGACAATAAAGATTCCACACCATTTGAGGATAATGAGGTGCAAGACGAATTTCTAGAGAAGCTGGAGACATCAGAATTCTTTGAGCTGACCTCAGAGGAGAAGCTACGGATATTGACAGCTCTTTGCCACCGTATCCTCATGACATACTCTGTGCAAGACCACATGGAGACAAGACAGCAGGTGTCTGCAGAGTTGTGGAAGGAGCGGCTTGCTGTGCTGAAGGAGGAGAATGATAAGAAGAGAGCAGAGAAGCAAAAGCGGAAGGAGATGGAAGCCAGAAATAAAGAAAATGGCAAAGAGGAGAATGTGCTAGGCAAAGTGGACAGGAAAAAAGAAATTGTGAAGATTGAACAGCAAGTAGAAGTGGAAGCAGATGACATGATCAGTGCTGTCAAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAACAAAAGATCATCAGCAATGTGCCAGCAGACAGCTTGATTCGAACGGAGAGACCACCAAATAA

Peptide sequence:

MTLNPSTKRRNTGSPDRKPSKKPKRDSSSLSSPLNPKLWCHVHLEKSLNGPPLKVKNSKNSKSPEEHLEGVMKIMSPNNNKLHSFHIPKKGPAAKKPGKHSDKPLKAKGRGKGILNGQKSTGNSKSPSKCVKTPKTKMKQMTLLDMAKGTQKMTRTPRSSGGVPRSSGKPHKHLPPAALHLIAYYKENKDKEDKKSALSCVISKTARLLSNEDRARLPEELRALVQKRYELLEHKKRWASMSEEQRKEYLKKKRQELKERLREKAKERREREMLERLEKQKRFEDQELGGRNLPAFRLVDTPEGLPNTLFGDVALVVEFLSCYSGLLLPDAQYPITAVSLMEALSADKGGFLYLNRVLVILLQTLLQDEIAEDYGELGMKLSEIPLTLHSVSELVRLCLRRCDVQEDSEGSETDDNKDSTPFEDNEVQDEFLEKLETSEFFELTSEEKLRILTALCHRILMTYSVQDHMETRQQVSAELWKERLAVLKEENDKKRAEKQKRKEMEARNKENGKEENVLGKVDRKKEIVKIEQQVEVEADDMISAVKSRRLLSMQAKRKREIQERETKDHQQCASRQLDSNGETTK

IRESfinder result

IRESfinder score

0.368341294

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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