top of page
mmu_circB3glct_730
Basic information of circular RNA
PanCircBase ID:
mmu_circB3glct_730
mm39_Chr. Position ID:
chr5|149626743|149663066|+
Gene name:
B3glct
Transcript:
ENSMUST00000100404.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
3,4,5,6,7,8,9,10
Exon/intron offsets:
0,6041,22132,23475,26645,29394,32091,36253
Genomic length:
36323
Exon/intron sizes in nt:
40,110,77,112,137,64,120,70
Flanking exon/introns:
chr5:149619939-149626743|chr5:149663066-149668890
Splice length:
730
Other database IDs:
circAtlas: mmu-B3glct_0007
Human Ortholog IDs:
Mature spliced Sequence:
GATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAGCAGAACAGTTGAAAAAAAACATCTTAAAGCAGGCTGCAAATCTGACACAGGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTACCGCTCTGTGGAGT
60.25
TGACTCTGGATGACAAACACGA
59.64
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCTACCGCTCTGTGGAGTA
59.38
TGACTCTGGATGACAAACACGA
59.64
158
PCR template sequence:
ACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGAAGATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-106b-5p(1), mmu-miR-24-3p(1), mmu-miR-384-3p(1), mmu-miR-3065-5p(1), mmu-miR-1904(1), mmu-miR-574-5p(1), mmu-miR-376a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATGCTGACAGAAGATTTGAGGCAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCB3GLCT_730_ORF_1
0.8747
0.157840463
0.912298074
741
ORF sequence:
ATGCTGACAGAAGATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAGCAGAACAGTTGAAAAAAAACATCTTAAAGCAGGCTGCAAATCTGACACAGGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGGAGTACCGGTAAAGAAGGAGGAGATTTTTGTTGCAGTGAAAACGTGCAAGAAATTTCATGCTGACAGA
Peptide sequence:
MLTEDLRQSSLPGRHDIDLKEIVFVIQSQSNSFHAKRAEQLKKNILKQAANLTQDLPRVLLLHQLAKQEGAWTILPLLPHFSVTYSKNSAWIFFCEEETRLQIPRLLDTLRRYDPSKEWFLGKALYDEESTIIHHYAFSENPTVFKYPDFAAGWALSIPLVNKLAKRLKSEALKSDFTIDLKHEIALYIWDKGGGPALTPVPEFCTEDVDPRCVTTFHSFLPLCGVPVKKEEIFVAVKTCKKFHADR
IRESfinder result
IRESfinder score
0.633019142
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page