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mmu_circB3glct_660
Basic information of circular RNA
PanCircBase ID:
mmu_circB3glct_660
mm39_Chr. Position ID:
chr5|149626743|149658954|+
Gene name:
B3glct
Transcript:
ENSMUST00000100404.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
3,4,5,6,7,8,9
Exon/intron offsets:
0,6041,22132,23475,26645,29394,32091
Genomic length:
32211
Exon/intron sizes in nt:
4,01,10,77,11,21,37,64,000
Flanking exon/introns:
chr5:149619939-149626743|chr5:149658954-149662996
Splice length:
660
Other database IDs:
CIRCpedia: MMU_CIRCpedia_10035, circAtlas: mmu-B3glct_0008
Human Ortholog IDs:
Mature spliced Sequence:
GATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAGCAGAACAGTTGAAAAAAAACATCTTAAAGCAGGCTGCAAATCTGACACAGGATCTCCCTAGAGTCCTCCTTCTCCATCAGTTGGCAAAGCAGGAAGGTGCTTGGACCATCCTTCCCTTGTTACCGCACTTTTCTGTAACATACAGCAAGAATTCAGCGTGGATTTTCTTCTGTGAAGAAGAGACGAGACTACAAATCCCCAGACTCCTAGACACACTCAGACGATATGACCCATCTAAGGAATGGTTTTTAGGAAAAGCACTGTATGACGAAGAGTCGACGATCATCCACCATTACGCCTTCTCTGAGAACCCAACAGTCTTTAAATATCCCGACTTCGCTGCTGGCTGGGCCCTTAGCATCCCGCTGGTGAACAAGCTTGCCAAAAGATTAAAGAGTGAGGCGCTGAAGTCTGACTTCACAATTGATTTAAAGCACGAGATTGCCCTCTACATCTGGGACAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCTGTGCCTGAGTTTTGT
60.47
TGACTCTGGATGACAAACACGA
59.64
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCCCTGTGCCTGAGTTTTG
60.47
TGACTCTGGATGACAAACACGA
59.64
152
PCR template sequence:
CAAAGGTGGAGGACCGGCCCTCACCCCTGTGCCTGAGTTTTGTACAGAAGATGTGGACCCCCGCTGCGTCACCACGTTCCATTCTTTCCTACCGCTCTGTGATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-106a-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-106b-5p(1), mmu-miR-24-3p(1), mmu-miR-384-3p(1), mmu-miR-1904(1), mmu-miR-574-5p(1), mmu-miR-376a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CCTACCGCTCTGTGATTTGAGGCAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.6455099
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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