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mmu_circB3glct_200
Basic information of circular RNA
PanCircBase ID:
mmu_circB3glct_200
mm39_Chr. Position ID:
chr5|149619889|149632894|+
Gene name:
B3glct
Transcript:
ENSMUST00000100404.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,6854,12895
Genomic length:
13005
Exon/intron sizes in nt:
50,40,110
Flanking exon/introns:
chr5:149601844-149619889|chr5:149632894-149648875
Splice length:
200
Other database IDs:
CIRCpedia: MMU_CIRCpedia_10031, circAtlas: mmu-B3glct_0002
circBase: hsa_circ_0002635, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_10528
Human Ortholog IDs:
Mature spliced Sequence:
CTTTTGCTTTGATGTCTGAAGAGATAAAAGAAAAGGTCACGCCTTCTCAGGATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAGCAGAACAGTTGAAAAAAAACATCTTAAAGCAGGCTGCAAATCTGACACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCAAGAGAGCAGAACAGT
59.24
TGTCATGTCTCCCCGGTAGA
59.67
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCAAGAGAGCAGAACAGT
59.24
TCATGTCTCCCCGGTAGACT
59.37
146
PCR template sequence:
AATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAGCAGAACAGTTGAAAAAAAACATCTTAAAGCAGGCTGCAAATCTGACACAGCTTTTGCTTTGATGTCTGAAGAGATAAAAGAAAAGGTCACGCCTTCTCAGGATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
AAATCTGACACAGCTTTTGCTTTGAT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCB3GLCT_200_ORF_1
0.9337
0.017196576
0.119694248
201
ORF sequence:
ATGTCTGAAGAGATAAAAGAAAAGGTCACGCCTTCTCAGGATTTGAGGCAGAGTAGTCTACCGGGGAGACATGACATAGACTTAAAAGAAATCGTGTTTGTCATCCAGAGTCAAAGTAATTCCTTTCATGCCAAGAGAGCAGAACAGTTGAAAAAAAACATCTTAAAGCAGGCTGCAAATCTGACACAGCTTTTGCTTTGA
Peptide sequence:
MSEEIKEKVTPSQDLRQSSLPGRHDIDLKEIVFVIQSQSNSFHAKRAEQLKKNILKQAANLTQLLL
IRESfinder result
IRESfinder score
0.546583266
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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