top of page
mmu_circAzi2_691
Basic information of circular RNA
PanCircBase ID:
mmu_circAzi2_691
mm39_Chr. Position ID:
chr9|117876480|117884978|+
Gene name:
Azi2
Transcript:
ENSMUST00000044454.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
2,3,4,5,6
Exon/intron offsets:
0,1897,2484,3941,8403
Genomic length:
8498
Exon/intron sizes in nt:
221,126,100,149,95
Flanking exon/introns:
chr9:117870026-117876480|chr9:117884978-117888124
Splice length:
691
Other database IDs:
circAtlas: mmu-Azi2_0008
Human Ortholog IDs:
Mature spliced Sequence:
CTGCCATGGACACGCTAGTAGAAGATGACATCTGCATTCTGAATCATGAAAAGGCCCACAGGAGAGAGGCAGTGACTCCACTCTCAGCGTACCCTGGAGATGAGTCTGTTGCTTCCCATTTCGCACTGGTCACTGCTTATGAAGACATCAAAAAGAGGCTGAAGGATTCAGAGAAAGAAAACTCCTTCTTAAAGAAAAGAATCAGGGCTTTGGAAGAAAGGCTTGTTGGAGCTCGAGCAGATGAAGAAACAAGTTCTGTGGGCCGAGAACAAGTGAATAAGGCCTATCATGCATATCGTGAGGTTTGCATTGATAGAGACAATCTGAAGAACCAATTGGAAAAGATAAATAAAGACAACTCTGAATCTTTGAAAATGTTGAATGAGCAGCTACAGTCTAAAGAAGTAGAACTTCTGCAGCTAAGAACAGAGGTGGAGACTCAGCAGGTGATGAGGAATTTAAACCCACCCTCATCCAGCTGGGAGGTGGAGAAGCTAAGCTGTGACTTGAAGATCCATGGTTTGGAGCAAGAGCTGGGACTACTGAGGAAGGAGTGCAGTGACCTCAGGACAGAGCTGCAGAAGGCCAGACAGACGGGTCCACCTCAGGAAGACATTCTGCAGGGCAGAGATGTCATCAGGCCGAGCTTGTCGAGAGAAGAGCATGTTCCACACCAGGGTCTTCACCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAAGACATTCTGCAGGGC
60.03
AGTCACTGCCTCTCTCCTGT
59.89
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGAAGACATTCTGCAGGGC
60.03
GTCACTGCCTCTCTCCTGTG
59.75
161
PCR template sequence:
AGACGGGTCCACCTCAGGAAGACATTCTGCAGGGCAGAGATGTCATCAGGCCGAGCTTGTCGAGAGAAGAGCATGTTCCACACCAGGGTCTTCACCACAGCTGCCATGGACACGCTAGTAGAAGATGACATCTGCATTCTGAATCATGAAAAGGCCCACAGGAGAGAGGCAGTGACTCCACTCTCAGCGTACCCTGGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-676-3p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-370-3p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCTTCACCACAGCTGCCATGGACAC
siRNA sets
TGGCAGCTGTGGTGAAGAC, ATGGCAGCTGTGGTGAAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAZI2_691_ORF_1
1.0075
0.253725306
0.954687063
750
ORF sequence:
ATGTCATCAGGCCGAGCTTGTCGAGAGAAGAGCATGTTCCACACCAGGGTCTTCACCACAGCTGCCATGGACACGCTAGTAGAAGATGACATCTGCATTCTGAATCATGAAAAGGCCCACAGGAGAGAGGCAGTGACTCCACTCTCAGCGTACCCTGGAGATGAGTCTGTTGCTTCCCATTTCGCACTGGTCACTGCTTATGAAGACATCAAAAAGAGGCTGAAGGATTCAGAGAAAGAAAACTCCTTCTTAAAGAAAAGAATCAGGGCTTTGGAAGAAAGGCTTGTTGGAGCTCGAGCAGATGAAGAAACAAGTTCTGTGGGCCGAGAACAAGTGAATAAGGCCTATCATGCATATCGTGAGGTTTGCATTGATAGAGACAATCTGAAGAACCAATTGGAAAAGATAAATAAAGACAACTCTGAATCTTTGAAAATGTTGAATGAGCAGCTACAGTCTAAAGAAGTAGAACTTCTGCAGCTAAGAACAGAGGTGGAGACTCAGCAGGTGATGAGGAATTTAAACCCACCCTCATCCAGCTGGGAGGTGGAGAAGCTAAGCTGTGACTTGAAGATCCATGGTTTGGAGCAAGAGCTGGGACTACTGAGGAAGGAGTGCAGTGACCTCAGGACAGAGCTGCAGAAGGCCAGACAGACGGGTCCACCTCAGGAAGACATTCTGCAGGGCAGAGATGTCATCAGGCCGAGCTTGTCGAGAGAAGAGCATGTTCCACACCAGGGTCTTCACCAC
Peptide sequence:
MSSGRACREKSMFHTRVFTTAAMDTLVEDDICILNHEKAHRREAVTPLSAYPGDESVASHFALVTAYEDIKKRLKDSEKENSFLKKRIRALEERLVGARADEETSSVGREQVNKAYHAYREVCIDRDNLKNQLEKINKDNSESLKMLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSSWEVEKLSCDLKIHGLEQELGLLRKECSDLRTELQKARQTGPPQEDILQGRDVIRPSLSREEHVPHQGLHH
IRESfinder result
IRESfinder score
0.501222023
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page