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mmu_circAU040320_1443
Basic information of circular RNA
PanCircBase ID:
mmu_circAU040320_1443
mm39_Chr. Position ID:
chr4|126729263|126741660|+
Gene name:
AU040320
Transcript:
ENSMUST00000102608.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
10,11,12,13,14,15,16,17,18,19
Exon/intron offsets:
0,1164,1816,4128,5175,5635,7005,10887,11670,12306
Genomic length:
12397
Exon/intron sizes in nt:
229,124,133,149,152,139,160,143,123,91
Flanking exon/introns:
chr4:126726015-126729263|chr4:126741660-126742603
Splice length:
1443
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTTAACTGTTGTCGACTCTGACGGGGCTACCAACTCCACCACTGCAAGCCTGACTGTGAACAAAGCTGTGGACTACCCTCCCGTGGCCAATGCAGGCCCCAACCAAGTGATCACCCTGCCTCAGAACTCCATCACCCTCTTTGGAAACCAGAGCACGGATGACCACGGCATCACCAGCTATGAGTGGTCGCTCAGCCCGAGCAGCAAAGGGAAGGTGGTGGAGATGCAGGGAGTTAGAACGCCAGCCCTGCAGCTGTCCGCAATGCAAGAAGGAGACTATACCTACCAGCTCACAGTGACTGACACCGCAGGACAACAGGCCACCGCCCAAGTGACTGTGATTGTGCAGCCTGAGAACAACAAGCCTCCTCAGGCAGATGCAGGCCCAGACAAAGAGCTGACCCTGCCCGTGGACAGCACAACCCTGGACGGCAGCAAGAGCACAGATGACCAGAGAGTCGTCTCTTACCTTTGGGAGCAGAGTCGGGGACCTGACGGGGTGCAGCTGGAGAATGCCAACAGCAGTGTCGCCACTGTGACTGGGCTGCAAGTCGGGACTTATGTATTCACCTTGACTGTCAAAGATGAGAGGAACCTACAGAGCCAGAGCTCCGTTAATGTCATTGTCAAAGAAGAAATAAACAAACCGCCAGTAGCCAAGATCGCTGGGAACGTGGTGGTGACCTTGCCCACGAGCACAGCTGAGCTGGATGGCTCGAGGTCCTCAGATGACAAGGGGATAGTCAGCTACCTGTGGACTCGAGATGAGACGAGCCCAGCCGCAGGGGAGGTGCTGAATCACTCTGACCACCACCCTGTCCTCTTCCTCTCCAACCTGGTGGAGGGGACCTACACGTTTCACCTGAAAGTGACAGATGCAAAGGGCGAGAGCGACACAGACCGGACGACAGTGGAAGTGAAGCCTGACCCCAGGAAAAGCAACCTAGTGGAGATCATCTTGGATGTGAACGTCAGTCAGCTGACTGAGAGGCTGAAGGGGATGCTCATCCGCCAGATTGGGGTCCTCCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGCAGAGCACCAAGATGTTGTTTTTTGTTCAGAACGACCCTCCCCACCAGCTCTTCAAAGGCCATGAGGTGGCAGCCATGCTCAAGAGCGAGCTGCAGAAGCAGAAGGCTGACTTCCTCATCTTCAGAGCCCTGGAAATCAGCACAGTCACATGTCAGCTGAACTGTTCTGACCATGGCCACTGTGACTCATTCACCAAGCGCTGTGTCTGTGACCCGTTTTGGATGGAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAATGGAGCGTGCTCTACGTCATCATTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGCTGCTGCAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAACTGTGAATGGAGCGTGC
60.11
TGTTCACAGTCAGGCTTGCA
60.11
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACTGTGAATGGAGCGTG
60.11
TGTTCACAGTCAGGCTTGCA
60.11
162
PCR template sequence:
GCAACTGTGAATGGAGCGTGCTCTACGTCATCATTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGCTGCTGCAAGAGCTTAACTGTTGTCGACTCTGACGGGGCTACCAACTCCACCACTGCAAGCCTGACTGTGAACAAAGCTGTGGACTACCCTCCCGTGGCCAATGCAGGCCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-134-5p(1), mmu-miR-15a-5p(1), mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-33-5p(1), mmu-miR-203-3p(1), mmu-miR-324-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
GCTGCTGCAAGAGCTTAACTGTTGTC
siRNA sets
AACAGTTAAGCTCTTGCAG, GTTAAGCTCTTGCAGCAGC, CAACAGTTAAGCTCTTGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAU040320_1443_ORF_1
1.3209
0.431801212
0.999999986
2661
ORF sequence:
ATGCAGGGAGTTAGAACGCCAGCCCTGCAGCTGTCCGCAATGCAAGAAGGAGACTATACCTACCAGCTCACAGTGACTGACACCGCAGGACAACAGGCCACCGCCCAAGTGACTGTGATTGTGCAGCCTGAGAACAACAAGCCTCCTCAGGCAGATGCAGGCCCAGACAAAGAGCTGACCCTGCCCGTGGACAGCACAACCCTGGACGGCAGCAAGAGCACAGATGACCAGAGAGTCGTCTCTTACCTTTGGGAGCAGAGTCGGGGACCTGACGGGGTGCAGCTGGAGAATGCCAACAGCAGTGTCGCCACTGTGACTGGGCTGCAAGTCGGGACTTATGTATTCACCTTGACTGTCAAAGATGAGAGGAACCTACAGAGCCAGAGCTCCGTTAATGTCATTGTCAAAGAAGAAATAAACAAACCGCCAGTAGCCAAGATCGCTGGGAACGTGGTGGTGACCTTGCCCACGAGCACAGCTGAGCTGGATGGCTCGAGGTCCTCAGATGACAAGGGGATAGTCAGCTACCTGTGGACTCGAGATGAGACGAGCCCAGCCGCAGGGGAGGTGCTGAATCACTCTGACCACCACCCTGTCCTCTTCCTCTCCAACCTGGTGGAGGGGACCTACACGTTTCACCTGAAAGTGACAGATGCAAAGGGCGAGAGCGACACAGACCGGACGACAGTGGAAGTGAAGCCTGACCCCAGGAAAAGCAACCTAGTGGAGATCATCTTGGATGTGAACGTCAGTCAGCTGACTGAGAGGCTGAAGGGGATGCTCATCCGCCAGATTGGGGTCCTCCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGCAGAGCACCAAGATGTTGTTTTTTGTTCAGAACGACCCTCCCCACCAGCTCTTCAAAGGCCATGAGGTGGCAGCCATGCTCAAGAGCGAGCTGCAGAAGCAGAAGGCTGACTTCCTCATCTTCAGAGCCCTGGAAATCAGCACAGTCACATGTCAGCTGAACTGTTCTGACCATGGCCACTGTGACTCATTCACCAAGCGCTGTGTCTGTGACCCGTTTTGGATGGAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAATGGAGCGTGCTCTACGTCATCATTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGCTGCTGCAAGAGCTTAACTGTTGTCGACTCTGACGGGGCTACCAACTCCACCACTGCAAGCCTGACTGTGAACAAAGCTGTGGACTACCCTCCCGTGGCCAATGCAGGCCCCAACCAAGTGATCACCCTGCCTCAGAACTCCATCACCCTCTTTGGAAACCAGAGCACGGATGACCACGGCATCACCAGCTATGAGTGGTCGCTCAGCCCGAGCAGCAAAGGGAAGGTGGTGGAGATGCAGGGAGTTAGAACGCCAGCCCTGCAGCTGTCCGCAATGCAAGAAGGAGACTATACCTACCAGCTCACAGTGACTGACACCGCAGGACAACAGGCCACCGCCCAAGTGACTGTGATTGTGCAGCCTGAGAACAACAAGCCTCCTCAGGCAGATGCAGGCCCAGACAAAGAGCTGACCCTGCCCGTGGACAGCACAACCCTGGACGGCAGCAAGAGCACAGATGACCAGAGAGTCGTCTCTTACCTTTGGGAGCAGAGTCGGGGACCTGACGGGGTGCAGCTGGAGAATGCCAACAGCAGTGTCGCCACTGTGACTGGGCTGCAAGTCGGGACTTATGTATTCACCTTGACTGTCAAAGATGAGAGGAACCTACAGAGCCAGAGCTCCGTTAATGTCATTGTCAAAGAAGAAATAAACAAACCGCCAGTAGCCAAGATCGCTGGGAACGTGGTGGTGACCTTGCCCACGAGCACAGCTGAGCTGGATGGCTCGAGGTCCTCAGATGACAAGGGGATAGTCAGCTACCTGTGGACTCGAGATGAGACGAGCCCAGCCGCAGGGGAGGTGCTGAATCACTCTGACCACCACCCTGTCCTCTTCCTCTCCAACCTGGTGGAGGGGACCTACACGTTTCACCTGAAAGTGACAGATGCAAAGGGCGAGAGCGACACAGACCGGACGACAGTGGAAGTGAAGCCTGACCCCAGGAAAAGCAACCTAGTGGAGATCATCTTGGATGTGAACGTCAGTCAGCTGACTGAGAGGCTGAAGGGGATGCTCATCCGCCAGATTGGGGTCCTCCTGGGGGTGCTGGATTCCGACATCATTGTGCAAAAGATTCAGCCGTACACGGAGCAGAGCACCAAGATGTTGTTTTTTGTTCAGAACGACCCTCCCCACCAGCTCTTCAAAGGCCATGAGGTGGCAGCCATGCTCAAGAGCGAGCTGCAGAAGCAGAAGGCTGACTTCCTCATCTTCAGAGCCCTGGAAATCAGCACAGTCACATGTCAGCTGAACTGTTCTGACCATGGCCACTGTGACTCATTCACCAAGCGCTGTGTCTGTGACCCGTTTTGGATGGAGAATTTCATCAAGGTGCAGCTGAGGGATGGAGACAGCAACTGTGAATGGAGCGTGCTCTACGTCATCATTGCCTCCTTTGTCATTGTTGTTGCCTTGGGGATCCTGTCATGGACTACAATCTGCTGCTGCAAG
Peptide sequence:
MQGVRTPALQLSAMQEGDYTYQLTVTDTAGQQATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSTDDQRVVSYLWEQSRGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPVAKIAGNVVVTLPTSTAELDGSRSSDDKGIVSYLWTRDETSPAAGEVLNHSDHHPVLFLSNLVEGTYTFHLKVTDAKGESDTDRTTVEVKPDPRKSNLVEIILDVNVSQLTERLKGMLIRQIGVLLGVLDSDIIVQKIQPYTEQSTKMLFFVQNDPPHQLFKGHEVAAMLKSELQKQKADFLIFRALEISTVTCQLNCSDHGHCDSFTKRCVCDPFWMENFIKVQLRDGDSNCEWSVLYVIIASFVIVVALGILSWTTICCCKSLTVVDSDGATNSTTASLTVNKAVDYPPVANAGPNQVITLPQNSITLFGNQSTDDHGITSYEWSLSPSSKGKVVEMQGVRTPALQLSAMQEGDYTYQLTVTDTAGQQATAQVTVIVQPENNKPPQADAGPDKELTLPVDSTTLDGSKSTDDQRVVSYLWEQSRGPDGVQLENANSSVATVTGLQVGTYVFTLTVKDERNLQSQSSVNVIVKEEINKPPVAKIAGNVVVTLPTSTAELDGSRSSDDKGIVSYLWTRDETSPAAGEVLNHSDHHPVLFLSNLVEGTYTFHLKVTDAKGESDTDRTTVEVKPDPRKSNLVEIILDVNVSQLTERLKGMLIRQIGVLLGVLDSDIIVQKIQPYTEQSTKMLFFVQNDPPHQLFKGHEVAAMLKSELQKQKADFLIFRALEISTVTCQLNCSDHGHCDSFTKRCVCDPFWMENFIKVQLRDGDSNCEWSVLYVIIASFVIVVALGILSWTTICCCK
IRESfinder result
IRESfinder score
0.348508336
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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