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mmu_circAtp8a2_1771
Basic information of circular RNA
PanCircBase ID:
mmu_circAtp8a2_1771
mm39_Chr. Position ID:
chr14|60028629|60263667|-
Gene name:
Atp8a2
Transcript:
ENSMUST00000080368.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
18
Exon/intron indexes:
29,28,27,26,25,24,23,22,21,20,19,18,17,16,15,14,13,12
Exon/intron offsets:
0,2686,11063,65848,68949,133826,206820,215048,217174,218622,221198,224693,225608,226379,229578,231942,232268,234960
Genomic length:
235038
Exon/intron sizes in nt:
79,123,75,111,184,173,65,139,140,85,70,50,83,106,76,45,89,78
Flanking exon/introns:
chr14:60011471-60028629|chr14:60263667-60264373
Splice length:
1771
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GACATGGATATGTATTATATTGAAAATGACACTCCTGCAATGGCCAGGACATCAAACCTCAACGAAGAGCTGGGGCAGGTAAAATACCTGTTTTCAGACAAGACTGGAACTCTTACATGTAATATCATGAACTTCAAGAAGTGTAGCATTGCTGGTGTGACCTATGGCCACTTCCCGGAACTAGCCAGAGAGCAGTCCTCAGATGACTTCTGCCGGATGACTTCTTGTACCAATGACTCATGTGACTTCAATGATCCCAGGCTGTTAAAGAACATCGAGGATCAGCACCCTACAGCCCCTTGCATACAAGAGTTCCTTACCCTGCTGGCTGTGTGTCACACCGTCGTCCCCGAGAAGGATGGAGATGAAATTATCTACCAAGCCTCGTCCCCAGATGAAGCTGCTTTGGTCAAAGGAGCTAAGAAGCTTGGCTTTGTGTTTACCGGGAGGACGCCGTACTCGGTCATCATCGAAGCGATGGGACAAGAACAGACATTCGGGATCCTCAATGTTCTAGAATTTTCTAGTGACAGGAAAAGAATGTCTGTCATTGTCCGACTGCCATCAGGACAACTTCGACTCTACTGCAAGGGAGCCGATAACGTCATCTTTGAAAGACTCTCAAAGGACTCAAAGTACATGGAGGAGACGCTATGCCATCTGGAATATTTTGCCACAGAAGGCCTGCGGACTCTGTGCGTGGCCTACGCAGACCTTTCTGAGAATGAGTATGAGGAGTGGCTGAAAGTCTATCAAGAGGCCAGCATCATTCTGAAGGACAGAGCCCAGAGGCTGGAAGAGTGTTACGAGATCATTGAGAAGAATTTACTGTTACTTGGAGCTACAGCCATCGAAGACCGTCTTCAAGCCGGCGTTCCAGAAACCATAGCCACTCTGCTGAAGGCAGAAATCAAAATCTGGGTGTTGACAGGAGACAAACAAGAAACTGCAATTAATATCGGGTATTCCTGTCGGTTGGTGTCGCAGAACATGGCCCTTATCCTATTGAAGGAGGACTCTTTAGATGCAACAAGGGCGGCCATCACTCAGCACTGCACAGATCTTGGAAATTTGCTGGGCAAAGAGAACGATGTGGCCCTCATCATCGATGGCCATACCCTGAAGTATGCACTCTCCTTTGAAGTCCGCAGAAGTTTCCTGGACCTGGCGCTCTCATGCAAAGCTGTCATCTGCTGCAGAGTGTCCCCTCTGCAGAAGTCTGAGATCGTGGATGTTGTGAAGAAGCGAGTGAAAGCCATCACCCTGGCCATCGGAGACGGTGCCAATGACGTGGGGATGATCCAGACAGCCCACGTAGGGGTCGGGATCAGCGGGAACGAGGGAATGCAGGCTACCAACAACTCAGATTATGCCATCGCACAGTTTTCCTACCTGGAGAAGCTGCTTTTGGTCCACGGAGCCTGGAGCTACAACCGGGTGACCAAGTGTATCCTGTACTGTTTCTACAAGAATGTGGTCCTCTACATCATCGAGCTATGGTTCGCCTTTGTGAATGGATTTTCTGGGCAGATTTTATTCGAGCGCTGGTGCATCGGCTTGTACAATGTGATCTTCACGGCATTGCCGCCCTTCACTCTGGGGATCTTCGAGAGGTCTTGTACTCAGGAGAGCATGCTCAGGTTCCCACAGCTTTACAGAATCACTCAGAACGCTGAAGGTTTCAACACTAAGGTTTTCTGGGGTCACTGCATCAATGCCTTGGTTCATTCCCTCATCCTCTTCTGGGTTCCCATGAAAGCGCTGGAGCATG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTTTTCTGGGGTCACTGC
60.18
CCCAGCTCTTCGTTGAGGTT
59.96
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGGTTTTCTGGGGTCACTGC
60.18
GAGGTTTGATGTCCTGGCCA
59.96
141
PCR template sequence:
GCTGAAGGTTTCAACACTAAGGTTTTCTGGGGTCACTGCATCAATGCCTTGGTTCATTCCCTCATCCTCTTCTGGGTTCCCATGAAAGCGCTGGAGCATGGACATGGATATGTATTATATTGAAAATGACACTCCTGCAATGGCCAGGACATCAAACCTCAACGAAGAGCTGGGGCAGGTAAAATACCTGTTTTCAGACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-23b-3p(1), mmu-miR-135a-5p(1), mmu-miR-101a-3p(1), mmu-miR-298-5p(1), mmu-miR-324-5p(1), mmu-miR-326-3p(1), mmu-miR-23a-3p(1), mmu-miR-501-3p(1), mmu-miR-194-5p(1), mmu-miR-382-5p(1), mmu-miR-224-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-300-3p(1), mmu-miR-5101(1), mmu-miR-369-3p(1), mmu-miR-3470b(1), mmu-miR-350-3p(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCGCTGGAGCATGGACATGGATATGT
siRNA sets
ATATCCATGTCCATGCTCC, ATCCATGTCCATGCTCCAG, ACATATCCATGTCCATGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCATP8A2_1771_ORF_1
1.2109
0.321151438
0.999985817
1791
ORF sequence:
ATGGATATGTATTATATTGAAAATGACACTCCTGCAATGGCCAGGACATCAAACCTCAACGAAGAGCTGGGGCAGGTAAAATACCTGTTTTCAGACAAGACTGGAACTCTTACATGTAATATCATGAACTTCAAGAAGTGTAGCATTGCTGGTGTGACCTATGGCCACTTCCCGGAACTAGCCAGAGAGCAGTCCTCAGATGACTTCTGCCGGATGACTTCTTGTACCAATGACTCATGTGACTTCAATGATCCCAGGCTGTTAAAGAACATCGAGGATCAGCACCCTACAGCCCCTTGCATACAAGAGTTCCTTACCCTGCTGGCTGTGTGTCACACCGTCGTCCCCGAGAAGGATGGAGATGAAATTATCTACCAAGCCTCGTCCCCAGATGAAGCTGCTTTGGTCAAAGGAGCTAAGAAGCTTGGCTTTGTGTTTACCGGGAGGACGCCGTACTCGGTCATCATCGAAGCGATGGGACAAGAACAGACATTCGGGATCCTCAATGTTCTAGAATTTTCTAGTGACAGGAAAAGAATGTCTGTCATTGTCCGACTGCCATCAGGACAACTTCGACTCTACTGCAAGGGAGCCGATAACGTCATCTTTGAAAGACTCTCAAAGGACTCAAAGTACATGGAGGAGACGCTATGCCATCTGGAATATTTTGCCACAGAAGGCCTGCGGACTCTGTGCGTGGCCTACGCAGACCTTTCTGAGAATGAGTATGAGGAGTGGCTGAAAGTCTATCAAGAGGCCAGCATCATTCTGAAGGACAGAGCCCAGAGGCTGGAAGAGTGTTACGAGATCATTGAGAAGAATTTACTGTTACTTGGAGCTACAGCCATCGAAGACCGTCTTCAAGCCGGCGTTCCAGAAACCATAGCCACTCTGCTGAAGGCAGAAATCAAAATCTGGGTGTTGACAGGAGACAAACAAGAAACTGCAATTAATATCGGGTATTCCTGTCGGTTGGTGTCGCAGAACATGGCCCTTATCCTATTGAAGGAGGACTCTTTAGATGCAACAAGGGCGGCCATCACTCAGCACTGCACAGATCTTGGAAATTTGCTGGGCAAAGAGAACGATGTGGCCCTCATCATCGATGGCCATACCCTGAAGTATGCACTCTCCTTTGAAGTCCGCAGAAGTTTCCTGGACCTGGCGCTCTCATGCAAAGCTGTCATCTGCTGCAGAGTGTCCCCTCTGCAGAAGTCTGAGATCGTGGATGTTGTGAAGAAGCGAGTGAAAGCCATCACCCTGGCCATCGGAGACGGTGCCAATGACGTGGGGATGATCCAGACAGCCCACGTAGGGGTCGGGATCAGCGGGAACGAGGGAATGCAGGCTACCAACAACTCAGATTATGCCATCGCACAGTTTTCCTACCTGGAGAAGCTGCTTTTGGTCCACGGAGCCTGGAGCTACAACCGGGTGACCAAGTGTATCCTGTACTGTTTCTACAAGAATGTGGTCCTCTACATCATCGAGCTATGGTTCGCCTTTGTGAATGGATTTTCTGGGCAGATTTTATTCGAGCGCTGGTGCATCGGCTTGTACAATGTGATCTTCACGGCATTGCCGCCCTTCACTCTGGGGATCTTCGAGAGGTCTTGTACTCAGGAGAGCATGCTCAGGTTCCCACAGCTTTACAGAATCACTCAGAACGCTGAAGGTTTCAACACTAAGGTTTTCTGGGGTCACTGCATCAATGCCTTGGTTCATTCCCTCATCCTCTTCTGGGTTCCCATGAAAGCGCTGGAGCATGGACATGGATATGTATTATATTGA
Peptide sequence:
MDMYYIENDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELAREQSSDDFCRMTSCTNDSCDFNDPRLLKNIEDQHPTAPCIQEFLTLLAVCHTVVPEKDGDEIIYQASSPDEAALVKGAKKLGFVFTGRTPYSVIIEAMGQEQTFGILNVLEFSSDRKRMSVIVRLPSGQLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGLRTLCVAYADLSENEYEEWLKVYQEASIILKDRAQRLEECYEIIEKNLLLLGATAIEDRLQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATRAAITQHCTDLGNLLGKENDVALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFTLGIFERSCTQESMLRFPQLYRITQNAEGFNTKVFWGHCINALVHSLILFWVPMKALEHGHGYVLY
IRESfinder result
IRESfinder score
0.406173257
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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