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mmu_circAsph_1037
Basic information of circular RNA
PanCircBase ID:
mmu_circAsph_1037
mm39_Chr. Position ID:
chr4|9529885|9610938|-
Gene name:
Asph
Transcript:
ENSMUST00000078139.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
17,16,15,14,13,12,11,10,9,8,7,6,5,4
Exon/intron offsets:
0,2029,7793,12339,53926,64777,65491,68383,68847,71421,72583,74630,77911,80957
Genomic length:
81053
Exon/intron sizes in nt:
137,151,87,86,45,36,39,33,48,57,33,114,75,96
Flanking exon/introns:
chr4:9517734-9529885|chr4:9610938-9635900
Splice length:
1037
Other database IDs:
CIRCpedia: MMU_CIRCpedia_35051, circAtlas: mmu-Asph_0027
Human Ortholog IDs:
Mature spliced Sequence:
GCCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGGAGGAGGCAGAGACTCACGCTGAGCTGGAGGAGCAGGCCCCTGAGGGGGCAGACATCCAGAATGTTGAAGATGAAGTAAAGGAACAAATTCAGTCCCTTCTTCAGGAATCAGTACACACAGACCATGACCTTGAAGCAGATGGGCTGGCAGGAGAACCACAGCCGGAGGTTGAGGACTTCCTCACAGTGACCGACAGTGATGACAGATTTGAGGACCTGGAACCCGGGACAGTTCATGAAGAAATCGAGGATACTTACCATGTGGAAGACACAGCATCGCAGAACCATCCAAATGACATGGAAGAGATGACGAATGAGCAGGAAAATTCAGATCCCAGTGAAGCAGTGACAGATGCAGGTGTGCTGCTGCCCCATGCAGAGGAAGTAAGACACCAAGACTATGATGAACCAGTATATGAACCCTCAGAGCATGAAGGGGTCGCGATTTCAGATAACACCATAGATGATTCCAGCATAATCTCAGAAGAAATAAATGTCGCCTCTGTTGAAGAACAGCAAGACACACCACCAGTTAAGAAGAAGAAGCCTAAACTTCTGAACAAATTTGATAAGACAATTAAGGCTGAGCTGGATGCTGCAGAAAAGCTCCGGAAAAGGGGTAAAATTGAGGAAGCAGTGAACGCATTTGAAGAACTGGTTCGAAAGTACCCTCAGAGCCCACGAGCAAGATATGGCAAAGCGCAGTGTGAAGATGACTTGGCAGAGAAGCAGAGAAGCAATGAGGTTCTGCGCAGGGCCATCGAGACCTACCAGGAGGCAGCCGACCTGCCTGATGCCCCTACAGACCTGGTGAAGCTGAGCTTGAAGCGAAGGTCGGAACGGCAGCAGTTTCTAGGTCACATGAGAGGTTCTCTACTTACCCTCCAGAGACTAGTTCAACTGTTCCCTAGTGATACTACCTTAAAAAACGACCTTGGCGTAGGATACCTCTTGTTGGGAGACAACGACAGTGCCAAGAAGGTTTACGAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGACCTTGGCGTAGGATACC
59.97
CTCCTCCAGCTCAGCGTG
59.82
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGACCTTGGCGTAGGATACC
59.97
CTCCTCCAGCTCAGCGTGAG
62.28
141
PCR template sequence:
AGTTCAACTGTTCCCTAGTGATACTACCTTAAAAAACGACCTTGGCGTAGGATACCTCTTGTTGGGAGACAACGACAGTGCCAAGAAGGTTTACGAAGAGGCCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGGAGGAGGCAGAGACTCACGCTGAGCTGGAGGAGCAGGCCCCTGAGGGGGCAGACAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-15b-5p(1), mmu-miR-328-3p(1), mmu-miR-196a-5p(1), mmu-miR-24-3p(1), mmu-miR-431-5p(1), mmu-miR-192-5p(1), mmu-miR-215-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGTTTACGAAGAGGCCTTAAAGAAAG
siRNA sets
TTTCTTTAAGGCCTCTTCG,TTCTTTAAGGCCTCTTCGT,CTTTCTTTAAGGCCTCTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASPH_1037_ORF_1
1.1334
0.299632999
0.981134671
819
ORF sequence:
ATGGAAGAGATGACGAATGAGCAGGAAAATTCAGATCCCAGTGAAGCAGTGACAGATGCAGGTGTGCTGCTGCCCCATGCAGAGGAAGTAAGACACCAAGACTATGATGAACCAGTATATGAACCCTCAGAGCATGAAGGGGTCGCGATTTCAGATAACACCATAGATGATTCCAGCATAATCTCAGAAGAAATAAATGTCGCCTCTGTTGAAGAACAGCAAGACACACCACCAGTTAAGAAGAAGAAGCCTAAACTTCTGAACAAATTTGATAAGACAATTAAGGCTGAGCTGGATGCTGCAGAAAAGCTCCGGAAAAGGGGTAAAATTGAGGAAGCAGTGAACGCATTTGAAGAACTGGTTCGAAAGTACCCTCAGAGCCCACGAGCAAGATATGGCAAAGCGCAGTGTGAAGATGACTTGGCAGAGAAGCAGAGAAGCAATGAGGTTCTGCGCAGGGCCATCGAGACCTACCAGGAGGCAGCCGACCTGCCTGATGCCCCTACAGACCTGGTGAAGCTGAGCTTGAAGCGAAGGTCGGAACGGCAGCAGTTTCTAGGTCACATGAGAGGTTCTCTACTTACCCTCCAGAGACTAGTTCAACTGTTCCCTAGTGATACTACCTTAAAAAACGACCTTGGCGTAGGATACCTCTTGTTGGGAGACAACGACAGTGCCAAGAAGGTTTACGAAGAGGCCTTAAAGAAAGATCTCCTTCTGAGAGGACATTTCCACCGGAGGAGGAGGCAGAGACTCACGCTGAGCTGGAGGAGCAGGCCCCTGAGGGGGCAGACATCCAGAATGTTGAAGATGAAGTAA
Peptide sequence:
MEEMTNEQENSDPSEAVTDAGVLLPHAEEVRHQDYDEPVYEPSEHEGVAISDNTIDDSSIISEEINVASVEEQQDTPPVKKKKPKLLNKFDKTIKAELDAAEKLRKRGKIEEAVNAFEELVRKYPQSPRARYGKAQCEDDLAEKQRSNEVLRRAIETYQEAADLPDAPTDLVKLSLKRRSERQQFLGHMRGSLLTLQRLVQLFPSDTTLKNDLGVGYLLLGDNDSAKKVYEEALKKDLLLRGHFHRRRRQRLTLSWRSRPLRGQTSRMLKMK
IRESfinder result
IRESfinder score
0.35992248
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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