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mmu_circAsh2l_192
Basic information of circular RNA
PanCircBase ID:
mmu_circAsh2l_192
mm39_Chr. Position ID:
chr8|26308573|26309746|-
Gene name:
Ash2l
Transcript:
ENSMUST00000068892.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
14,13
Exon/intron offsets:
0,1080
Genomic length:
1173
Exon/intron sizes in nt:
99,93
Flanking exon/introns:
chr8:26307691-26308573|chr8:26309746-26312722
Splice length:
192
Other database IDs:
circBase: hsa_circ_0136476, circAtlas: hsa-ASH2L_0028, CIRCpedia: HSA_CIRCpedia_60953
Human Ortholog IDs:
Mature spliced Sequence:
GCTTTGATAAAGTTCAAGAGTTATTTGTATTTTGAAGAAAAAGACTTTGTGGACAAAGCAGAGAAGAGCCTAAAACAGACCCCCCATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAGGGTGTGGCTTACAGAGATATTTTTGAAGGAGTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTGTCAATCAGGGTGTGG
59.89
GGGGGTCTGTTTTAGGCTCT
59.01
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTGTCAATCAGGGTGTGG
59.89
AGGCTCTTCTCTGCTTTGTCC
60
153
PCR template sequence:
ATATTTTATAAAAATGGTGTCAATCAGGGTGTGGCTTACAGAGATATTTTTGAAGGAGTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGCTTTGATAAAGTTCAAGAGTTATTTGTATTTTGAAGAAAAAGACTTTGTGGACAAAGCAGAGAAGAGCCTAAAACAGACCCCCCATAGTGAGATA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
CAAGAGCTGCACGGCTTTGATAAAGT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASH2L_192_ORF_1
0.7461
-0.28573244
0.012329166
90
ORF sequence:
ATGGTGTCAATCAGGGTGTGGCTTACAGAGATATTTTTGAAGGAGTTTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGCTTTGA
Peptide sequence:
MVSIRVWLTEIFLKEFTSQPSHCTRAARL
IRESfinder result
IRESfinder score
0.842495812
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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