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mmu_circAsh1l_739
Basic information of circular RNA
PanCircBase ID:
mmu_circAsh1l_739
mm39_Chr. Position ID:
chr3|88973478|88977953|+
Gene name:
Ash1l
Transcript:
ENSMUST00000186583.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
20,21,22,23,24
Exon/intron offsets:
0,983,2815,4056,4302
Genomic length:
4475
Exon/intron sizes in nt:
24,98,79,11,39,173
Flanking exon/introns:
chr3:88971225-88973478|chr3:88977953-88979752
Splice length:
739
Other database IDs:
CIRCpedia: MMU_CIRCpedia_33323, circAtlas: mmu-Ash1l_0044
circBase: hsa_circ_0110869, circAtlas: hsa-ASH1L_0041, CIRCpedia: HSA_CIRCpedia_28163
Human Ortholog IDs:
Mature spliced Sequence:
AAGTACTATGGGCGTAAATCCCCAATTGGGAGAGATGTTTGCCGCTTACGAAAGGCCTATTACAGCGCCCGGCATGAAGCTTCGGCTCAGATCGATGAGATTGTGGGAGAAACGGCCAGTGAGGCCGACAGCAGCGAGACCTCAGTCTCCGAGAAGGAGAGCGGGCACGAGAAGGATGATGATGTTATCCGCTGCATCTGCGGCCTCTATAAAGATGAAGGCCTCATGATCCAGTGTGACAAGTGCATGGTGTGGCAGCACTGCGACTGCATGGGTGTGAACACGGATGTGGAGCACTACCTTTGTGAGCAGTGTGACCCTCGGCCTGTGGACAGGGAGGTACCCATGATCCCTAGGCCCCACTATGCCCAGCCCGGCTGTGTCTACTTCATCTGTTTACTCCGAGATGACTTGCTGCTTCGTCAGGGTGATTGTGTGTACCTGATGAGGGACAGTCGGCGCACGCCTGATGGCCACCCAGTCCGTCAGTCCTACAGACTCTTATCTCACATTAACCGAGATAAACTTGACATCTTTAGAATCGAGAAGCTCTGGAAGAATGAAAAAGAAGAACGGTTTGCCTTTGGTCACCATTATTTCCGCCCACATGAGACTCATCACTCTCCATCCCGTCGGTTCTATCACAACGAGCTGTTCCGGGTGCCCCTCTATGAGATCATTCCCTTGGAGGCCGTAGTTGGGACCTGCTGTGTGTTGGACCTTTATACATACTGTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATTCCCTTGGAGGCCGTAG
60.18
TCATCGATCTGAGCCGAAGC
59.97
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TAGTTGGGACCTGCTGTGTG
59.6
TCATCGATCTGAGCCGAAGC
59.97
143
PCR template sequence:
TATCACAACGAGCTGTTCCGGGTGCCCCTCTATGAGATCATTCCCTTGGAGGCCGTAGTTGGGACCTGCTGTGTGTTGGACCTTTATACATACTGTAAAGAAGTACTATGGGCGTAAATCCCCAATTGGGAGAGATGTTTGCCGCTTACGAAAGGCCTATTACAGCGCCCGGCATGAAGCTTCGGCTCAGATCGATGAGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-101b-3p(1), mmu-miR-196a-5p(1), mmu-miR-339-5p(1), mmu-miR-30d-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACATACTGTAAAGAAGTACTATGGGC
siRNA sets
ATAGTACTTCTTTACAGTA,CATAGTACTTCTTTACAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASH1L_739_ORF_1
0.7852
0.252349179
0.740030399
531
ORF sequence:
ATGATCCAGTGTGACAAGTGCATGGTGTGGCAGCACTGCGACTGCATGGGTGTGAACACGGATGTGGAGCACTACCTTTGTGAGCAGTGTGACCCTCGGCCTGTGGACAGGGAGGTACCCATGATCCCTAGGCCCCACTATGCCCAGCCCGGCTGTGTCTACTTCATCTGTTTACTCCGAGATGACTTGCTGCTTCGTCAGGGTGATTGTGTGTACCTGATGAGGGACAGTCGGCGCACGCCTGATGGCCACCCAGTCCGTCAGTCCTACAGACTCTTATCTCACATTAACCGAGATAAACTTGACATCTTTAGAATCGAGAAGCTCTGGAAGAATGAAAAAGAAGAACGGTTTGCCTTTGGTCACCATTATTTCCGCCCACATGAGACTCATCACTCTCCATCCCGTCGGTTCTATCACAACGAGCTGTTCCGGGTGCCCCTCTATGAGATCATTCCCTTGGAGGCCGTAGTTGGGACCTGCTGTGTGTTGGACCTTTATACATACTGTAAAGAAGTACTATGGGCGTAA
Peptide sequence:
MIQCDKCMVWQHCDCMGVNTDVEHYLCEQCDPRPVDREVPMIPRPHYAQPGCVYFICLLRDDLLLRQGDCVYLMRDSRRTPDGHPVRQSYRLLSHINRDKLDIFRIEKLWKNEKEERFAFGHHYFRPHETHHSPSRRFYHNELFRVPLYEIIPLEAVVGTCCVLDLYTYCKEVLWA
IRESfinder result
IRESfinder score
0.396389568
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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