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mmu_circAsh1l_736
Basic information of circular RNA
PanCircBase ID:
mmu_circAsh1l_736
mm39_Chr. Position ID:
chr3|88914448|88915184|+
Gene name:
Ash1l
Transcript:
ENSMUST00000186583.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
5
Exon/intron offsets:
0
Genomic length:
736
Exon/intron sizes in nt:
736
Flanking exon/introns:
chr3:88909181-88914448|chr3:88915184-88930337
Splice length:
736
Other database IDs:
circBase: mmu_circ_0010877, CIRCpedia: MMU_CIRCpedia_33272, circAtlas: mmu-Ash1l_0018
circBase: hsa_circ_0000137, circAtlas: hsa-ASH1L_0016, CIRCpedia: HSA_CIRCpedia_28184
Human Ortholog IDs:
Mature spliced Sequence:
TGAATGGAGTTCCTTCCAGAAGTCCCAGATTGGTTGCTTCTATGGATGATTCTGTAGACAGTCTGTTGCAGCGGATAGTACATCACGATGAGCAAGAGTCCATGGAGAAAAACGGTGATGCTTCCATTACAACTGTGTCTGCACCACCTTCTTCCAGTCCAGGCCATAGCTACAGCAAGGAGCGAGCCTTAGGTAAATCAGACAGCCTTCTAGTGCCTGCAGTCCCGAATGACTCTTGCAGTAATATCCCACTTCTTTCTGAAAAGTCAGCAAGTAGGTGTTCCCCCCATCACATCAAGAGAAGTGTAGTTGAAGCTATGCAACGCCAAGCTCGGAAAATGTGCAATTATGACAAAATCTTGGCCACCAAGAAAAACCTGGACCATGTTAATAAAATTTTAAAAGCCAAAAAACTTCAAAGGCAGGCCAGGACAGGAAACAATTTTGTGAAACGCAGACCAGGTCGACCTCGGAAATGCCCCCTCCAAGCAGTGGTGTCAATGCAAGCCTTCCAGGCCGCCCAGTTTGTCAGCCCGGAATTGAATGAAGGCGAAGACATGTCCCTGCACCTAAGTCCAGACACAGTCACTGATGTGATCGAGGCTGTGGTTCAGAGTGTGAACCTCACTTCAGAACATAAGAAGGGGGTGAAGAGGAAAAATTGGCTGTTAGAAGAACAGACTAGGAAAAAGCAGAAGACAGTACCAGAGGAAGAAGAGCAAGAAAACAATAAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGGTGAAGAGGAAAAATTGGC
60.03
CCGCTGCAACAGACTGTCTA
60.04
166
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAAGGGGGTGAAGAGGAAA
57.87
CCGCTGCAACAGACTGTCTA
60.04
171
PCR template sequence:
ATAAGAAGGGGGTGAAGAGGAAAAATTGGCTGTTAGAAGAACAGACTAGGAAAAAGCAGAAGACAGTACCAGAGGAAGAAGAGCAAGAAAACAATAAAAGTGAATGGAGTTCCTTCCAGAAGTCCCAGATTGGTTGCTTCTATGGATGATTCTGTAGACAGTCTGTTGCAGCGGATAGTACATCACGATGAGCAAGAGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-134-5p(1), mmu-miR-32-5p(1), mmu-miR-92a-3p(1), mmu-miR-205-5p(1), mmu-miR-25-3p(1), mmu-miR-363-3p(1), mmu-miR-153-3p(1), mmu-miR-384-3p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
AAAACAATAAAAGTGAATGGAGTTCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASH1L_736_ORF_1
0.8961
0.171147515
0.921115148
744
ORF sequence:
ATGGATGATTCTGTAGACAGTCTGTTGCAGCGGATAGTACATCACGATGAGCAAGAGTCCATGGAGAAAAACGGTGATGCTTCCATTACAACTGTGTCTGCACCACCTTCTTCCAGTCCAGGCCATAGCTACAGCAAGGAGCGAGCCTTAGGTAAATCAGACAGCCTTCTAGTGCCTGCAGTCCCGAATGACTCTTGCAGTAATATCCCACTTCTTTCTGAAAAGTCAGCAAGTAGGTGTTCCCCCCATCACATCAAGAGAAGTGTAGTTGAAGCTATGCAACGCCAAGCTCGGAAAATGTGCAATTATGACAAAATCTTGGCCACCAAGAAAAACCTGGACCATGTTAATAAAATTTTAAAAGCCAAAAAACTTCAAAGGCAGGCCAGGACAGGAAACAATTTTGTGAAACGCAGACCAGGTCGACCTCGGAAATGCCCCCTCCAAGCAGTGGTGTCAATGCAAGCCTTCCAGGCCGCCCAGTTTGTCAGCCCGGAATTGAATGAAGGCGAAGACATGTCCCTGCACCTAAGTCCAGACACAGTCACTGATGTGATCGAGGCTGTGGTTCAGAGTGTGAACCTCACTTCAGAACATAAGAAGGGGGTGAAGAGGAAAAATTGGCTGTTAGAAGAACAGACTAGGAAAAAGCAGAAGACAGTACCAGAGGAAGAAGAGCAAGAAAACAATAAAAGTGAATGGAGTTCCTTCCAGAAGTCCCAGATTGGTTGCTTCTATGGATGA
Peptide sequence:
MDDSVDSLLQRIVHHDEQESMEKNGDASITTVSAPPSSSPGHSYSKERALGKSDSLLVPAVPNDSCSNIPLLSEKSASRCSPHHIKRSVVEAMQRQARKMCNYDKILATKKNLDHVNKILKAKKLQRQARTGNNFVKRRPGRPRKCPLQAVVSMQAFQAAQFVSPELNEGEDMSLHLSPDTVTDVIEAVVQSVNLTSEHKKGVKRKNWLLEEQTRKKQKTVPEEEEQENNKSEWSSFQKSQIGCFYG
IRESfinder result
IRESfinder score
0.405477745
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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