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mmu_circAscc3_283
Basic information of circular RNA
PanCircBase ID:
mmu_circAscc3_283
mm39_Chr. Position ID:
chr10|50480846|50484166|+
Gene name:
Ascc3
Transcript:
ENSMUST00000035606.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,3169
Genomic length:
3320
Exon/intron sizes in nt:
132,151
Flanking exon/introns:
chr10:50468993-50480846|chr10:50484166-50493807
Splice length:
283
Other database IDs:
CIRCpedia: MMU_CIRCpedia_21882, circAtlas: mmu-Ascc3_0047
circAtlas: hsa-ASCC3_0038, CIRCpedia: HSA_CIRCpedia_146758
Human Ortholog IDs:
Mature spliced Sequence:
TTTCGAGTTTCTTATTTCTATGGATCACTGACTGAGTTATAAATGGCTCTACCTCGGCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAGATAATTACAATGAAGAAGTAGCTGACTTGAAGTTGAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTTGGCCTTACATGGAAGAAGATAGTGAAATTTTTGAATGAAAAGCTAGAGAAGAATAAAATGCAAAATATAAATGAAGACTTAAAAGACATACTACAAGCTGCAAAACAAATCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAAAGCTAGAGAAGAATAAAATGCA
57.99
TTTGAAAAGGAGCGCAAGGC
59.97
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAAAGCTAGAGAAGAATAAAATGCA
57.99
TCCTGTGAGCCGAGGTAGAG
60.39
143
PCR template sequence:
AAGAAGATAGTGAAATTTTTGAATGAAAAGCTAGAGAAGAATAAAATGCAAAATATAAATGAAGACTTAAAAGACATACTACAAGCTGCAAAACAAATCGTTTCGAGTTTCTTATTTCTATGGATCACTGACTGAGTTATAAATGGCTCTACCTCGGCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAAAACAAATCGTTTCGAGTTTCTT
siRNA sets
GAAACTCGAAACGATTTGT, AAACTCGAAACGATTTGTT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASCC3_283_ORF_1
0.7253
-0.014430044
0.121868192
276
ORF sequence:
ATGGCTCTACCTCGGCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAGATAATTACAATGAAGAAGTAGCTGACTTGAAGTTGAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTTGGCCTTACATGGAAGAAGATAGTGAAATTTTTGAATGAAAAGCTAGAGAAGAATAAAATGCAAAATATAAATGAAGACTTAAAAGACATACTACAAGCTGCAAAACAAATCGTTTCGAGTTTCTTATTTCTATGGATCACTGACTGA
Peptide sequence:
MALPRLTGALRSFSNVTKQDNYNEEVADLKLKRSKLHEQVLDFGLTWKKIVKFLNEKLEKNKMQNINEDLKDILQAAKQIVSSFLFLWITD
IRESfinder result
IRESfinder score
0.629573377
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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