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mmu_circAscc3_1538
Basic information of circular RNA
PanCircBase ID:
mmu_circAscc3_1538
mm39_Chr. Position ID:
chr10|50625130|50725742|+
Gene name:
Ascc3
Transcript:
ENSMUST00000035606.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
32,33,34,35,36,37,38,39,40,41
Exon/intron offsets:
0,1418,4962,18056,18338,74601,91263,93108,96496,100436
Genomic length:
100612
Exon/intron sizes in nt:
1,21,13,31,26,11,21,35,00,00,00,00,00,00,000
Flanking exon/introns:
chr10:50625039-50625130|chr10:50725742-50726556
Splice length:
1538
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTCTCATTGCTACAAGCACATTAGCATGGGGTGTAAACTTTCCAGCACACTTAGTAATTATTAAGGGAACTGAATATTATGATGGAAAGACAAGACGTTATGTGGATTTTCCCATCACAGATGTGCTGCAGATGATGGGACGAGCTGGGAGACCTCAGTTTGATGACCAGGGCAAAGCTGTAATTCTAGTTCATGATATAAAAAAAGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAGTAGAATCAAGCTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCTGGAGGTACAATAACATCAAAGCAAGATGCAATGGATTATATCACCTGGACTTACTTTTTCCGGCGTCTTATCATGAATCCCAGCTACTACAGCTTGGGTGACGTCAGCCAGGACTCCATAAACAAGTTCCTGTCACATCTGATTGGGCAGTCTCTGGTTGAATTGGAGCTTTCCCACTGCATTGAAGTTGGAGAGGATAATCGCACCATTGAACCACTGACATGTGGTCGAATTGCCTCCTATTACTATTTGAAGCACAAAACTGTCAAAATGTTCAAGGACCGTTTGAAACCTGAGTGCAGCACTGAGGAACTGCTGTCAATTCTGAGTGATGCAGAAGAATACACAGACTTACCAGTAAGACACAATGAGGACCATACGAATAATGAATTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAGCCCTCACACCAAAGCCCATCTCTTGCTTCAAGCGCATCTCAGCCGAGCCATGCTGCCCTGCCCAGATTATGATACTGATACCAAAACAGTCTTAGACCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAAGCCAGGGATGGCTAGTGACCGTCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCTCAAGGACTCTTCTCTTCTCACAATACCAAACATAGAGCAGCACCACCTTCACCTTTTCAGGAAATGGAAGCCACCTGTAAAGAGCTCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCTGTGAAGGGAAGGACCATGTGTTCAGCTCCATGGTAGAGAAAGAGTTACAGCCTGCAAAAACAAAGCAGGCATGGAATTTCTTATCTCGCTTGCCAGTGATAAATGTTGGCATAAGTGTTAAAGGCTCATGGGATGACTTAGTTGAAGGACATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACAAATGGATCAAGTTGCATGCTGATCAGGAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGGGCTTCCAAAGGGAAAACATGAGAATCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGCTGGTTTTTGATATTAGGAGAAGTGGATAAGAGAGAGCTGATGGCTGTAAAACGAGTAGGATTTGTTCGAACACATCATGATGCTTCCATCTCGTTCTTTACTCCTGAAACACCTGGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGATAAGAGAGAGCTGATGGC
59.37
TGCTGGAAAGTTTACACCCCA
59.78
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGATAAGAGAGAGCTGATGGC
59.37
GTGCTGGAAAGTTTACACCCC
59.39
146
PCR template sequence:
AAGTGGATAAGAGAGAGCTGATGGCTGTAAAACGAGTAGGATTTGTTCGAACACATCATGATGCTTCCATCTCGTTCTTTACTCCTGAAACACCTGGAAGGTTCTCATTGCTACAAGCACATTAGCATGGGGTGTAAACTTTCCAGCACACTTAGTAATTATTAAGGGAACTGAATATTATGATGGAAAGACAAGACGTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-433-3p(2), mmu-miR-124-3p(1), mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-7a-5p(1), mmu-miR-136-5p(1), mmu-miR-107-3p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-540-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAACACCTGGAAGGTTCTCATTGCTA
siRNA sets
TAGCAATGAGAACCTTCCA, GCAATGAGAACCTTCCAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASCC3_1538_ORF_1
0.8868
0.097736183
0.999142216
1431
ORF sequence:
ATGATGGGACGAGCTGGGAGACCTCAGTTTGATGACCAGGGCAAAGCTGTAATTCTAGTTCATGATATAAAAAAAGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAGTAGAATCAAGCTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCTGGAGGTACAATAACATCAAAGCAAGATGCAATGGATTATATCACCTGGACTTACTTTTTCCGGCGTCTTATCATGAATCCCAGCTACTACAGCTTGGGTGACGTCAGCCAGGACTCCATAAACAAGTTCCTGTCACATCTGATTGGGCAGTCTCTGGTTGAATTGGAGCTTTCCCACTGCATTGAAGTTGGAGAGGATAATCGCACCATTGAACCACTGACATGTGGTCGAATTGCCTCCTATTACTATTTGAAGCACAAAACTGTCAAAATGTTCAAGGACCGTTTGAAACCTGAGTGCAGCACTGAGGAACTGCTGTCAATTCTGAGTGATGCAGAAGAATACACAGACTTACCAGTAAGACACAATGAGGACCATACGAATAATGAATTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAGCCCTCACACCAAAGCCCATCTCTTGCTTCAAGCGCATCTCAGCCGAGCCATGCTGCCCTGCCCAGATTATGATACTGATACCAAAACAGTCTTAGACCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAAGCCAGGGATGGCTAGTGACCGTCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCTCAAGGACTCTTCTCTTCTCACAATACCAAACATAGAGCAGCACCACCTTCACCTTTTCAGGAAATGGAAGCCACCTGTAAAGAGCTCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCTGTGAAGGGAAGGACCATGTGTTCAGCTCCATGGTAGAGAAAGAGTTACAGCCTGCAAAAACAAAGCAGGCATGGAATTTCTTATCTCGCTTGCCAGTGATAAATGTTGGCATAAGTGTTAAAGGCTCATGGGATGACTTAGTTGAAGGACATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACAAATGGATCAAGTTGCATGCTGATCAGGAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGGGCTTCCAAAGGGAAAACATGAGAATCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGCTGGTTTTTGATATTAGGAGAAGTGGATAAGAGAGAGCTGATGGCTGTAAAACGAGTAGGATTTGTTCGAACACATCATGATGCTTCCATCTCGTTCTTTACTCCTGAAACACCTGGAAGGTTCTCATTGCTACAAGCACATTAG
Peptide sequence:
MMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLGVLSDHLNAEIAGGTITSKQDAMDYITWTYFFRRLIMNPSYYSLGDVSQDSINKFLSHLIGQSLVELELSHCIEVGEDNRTIEPLTCGRIASYYYLKHKTVKMFKDRLKPECSTEELLSILSDAEEYTDLPVRHNEDHTNNELAKCLPIELNPHSFDSPHTKAHLLLQAHLSRAMLPCPDYDTDTKTVLDQALRVCQAMLDVAASQGWLVTVLNITHLIQMVIQGRWLKDSSLLTIPNIEQHHLHLFRKWKPPVKSSHAKCRTSIECLPELIHACEGKDHVFSSMVEKELQPAKTKQAWNFLSRLPVINVGISVKGSWDDLVEGHNELSISTLTADKRDENKWIKLHADQEYVLQVSLQRVHFGLPKGKHENHAVTPRFPKLKDEGWFLILGEVDKRELMAVKRVGFVRTHHDASISFFTPETPGRFSLLQAH
IRESfinder result
IRESfinder score
0.594444018
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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