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mmu_circAsb8_269
Basic information of circular RNA
PanCircBase ID:
mmu_circAsb8_269
mm39_Chr. Position ID:
chr15|98039164|98040695|-
Gene name:
Asb8
Transcript:
ENSMUST00000143400.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,1367
Genomic length:
1531
Exon/intron sizes in nt:
1,05,164
Flanking exon/introns:
chr15:98034319-98039164|chr15:98040695-98063351
Splice length:
269
Other database IDs:
circAtlas: mmu-Asb8_0001
circAtlas: hsa-ASB8_0001, CIRCpedia: HSA_CIRCpedia_8549
Human Ortholog IDs:
Mature spliced Sequence:
GCAGTTTGGAGCATGTGAACACACTCTGAGCCTTGATGAGTTCCAGCATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCCCTCTCCGAACGCCTGATCCGAACCATCGCTGCCATCCGCTCCTTCCCACATGACAACGTAGAGGACCTCATCAGAGGGGGAGCAGATGTGAACTGCACGCACGGCACACTGAAGCCCCTCCACTGTGCCTGCATGGTGTCCGACGCCGACTGCGTAGAGCTCCTCCTGGAGAAGGGAGCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGAAGCCCCTCCACTGTG
60
GCGTTCGGAGAGGGAGTATT
59.25
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACTGAAGCCCCTCCACTG
60
GCGTTCGGAGAGGGAGTATT
59.25
175
PCR template sequence:
AGATGTGAACTGCACGCACGGCACACTGAAGCCCCTCCACTGTGCCTGCATGGTGTCCGACGCCGACTGCGTAGAGCTCCTCCTGGAGAAGGGAGCAGAGGCAGTTTGGAGCATGTGAACACACTCTGAGCCTTGATGAGTTCCAGCATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCCCTCTCCGAACGCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-128-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGGGAGCAGAGGCAGTTTGGAGCA
siRNA sets
TCCAAACTGCCTCTGCTCC, CAAACTGCCTCTGCTCCCT, TGCTCCAAACTGCCTCTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASB8_269_ORF_1
1.0632
0.352011814
0.494802843
252
ORF sequence:
ATGAGTTCCAGCATGTGGTATATTATGCAGAGCATTCAGAGCAAATACTCCCTCTCCGAACGCCTGATCCGAACCATCGCTGCCATCCGCTCCTTCCCACATGACAACGTAGAGGACCTCATCAGAGGGGGAGCAGATGTGAACTGCACGCACGGCACACTGAAGCCCCTCCACTGTGCCTGCATGGTGTCCGACGCCGACTGCGTAGAGCTCCTCCTGGAGAAGGGAGCAGAGGCAGTTTGGAGCATGTGA
Peptide sequence:
MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGGADVNCTHGTLKPLHCACMVSDADCVELLLEKGAEAVWSM
IRESfinder result
IRESfinder score
0.320543604
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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