top of page
mmu_circAsap2_612
Basic information of circular RNA
PanCircBase ID:
mmu_circAsap2_612
mm39_Chr. Position ID:
chr12|21267975|21280957|+
Gene name:
Asap2
Transcript:
ENSMUST00000064595.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
11,12,13,14,15,16
Exon/intron offsets:
0,213,6330,11264,11399,12848
Genomic length:
12982
Exon/intron sizes in nt:
104,70,88,49,167,134
Flanking exon/introns:
chr12:21264371-21267975|chr12:21280957-21289555
Splice length:
612
Other database IDs:
circBase: mmu_circ_0003834, CIRCpedia: MMU_CIRCpedia_13170, circAtlas: mmu-Asap2_0004
circBase: hsa_circ_0052527, circAtlas: hsa-ASAP2_0005, CIRCpedia: HSA_CIRCpedia_42606
Human Ortholog IDs:
Mature spliced Sequence:
GACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGCATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCAGAAGAGGAAGTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCCAAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCACATGACAGGACGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAACAGCAAGGAAGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACCAAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATCCGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CATCGAGTGCTCTGGGATCC
59.97
AGGTTCCCGTTCCTCTCTGT
60.18
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CATCGAGTGCTCTGGGATCC
59.97
TCCCGTTCCTCTCTGTTCCA
60.18
176
PCR template sequence:
GACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTGGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGCATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-375-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-217-5p(1), mmu-miR-125a-5p(1), mmu-miR-676-3p(1), mmu-miR-705(1), mmu-miR-29b-2-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCTGAGCTTCTGGACTCACAACTTC
siRNA sets
AAGTTGTGAGTCCAGAAGC, GAAGTTGTGAGTCCAGAAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAP2_612_ORF_1
1.1321
0.432705714
0.994304489
909
ORF sequence:
ATGTCTGTACTGCAGAACAGCAAGGAAGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACCAAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATCCGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTGGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGCATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCAGAAGAGGAAGTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCCAAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCACATGACAGGACGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAACAGCAAGGAAGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACCAAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATCCGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTG
Peptide sequence:
MSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLDSQLRQSTAYSLHQPQGNKEHGTERNGNLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELL
IRESfinder result
IRESfinder score
0.251935534
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page