top of page
mmu_circAsap2_495
Basic information of circular RNA
PanCircBase ID:
mmu_circAsap2_495
mm39_Chr. Position ID:
chr12|21249544|21263226|+
Gene name:
Asap2
Transcript:
ENSMUST00000064595.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
4,5,6,7,8,9
Exon/intron offsets:
0,1336,4416,6759,13265,13604
Genomic length:
13682
Exon/intron sizes in nt:
75,50,130,86,76,78
Flanking exon/introns:
chr12:21235244-21249544|chr12:21263226-21264362
Splice length:
495
Other database IDs:
circBase: hsa_circ_0121185, circAtlas: hsa-ASAP2_0022, CIRCpedia: HSA_CIRCpedia_42598
Human Ortholog IDs:
Mature spliced Sequence:
ATTCAGAACATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGGGATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGAAAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGATGGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTGAAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATGGATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAACAGAAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCGACAGCTGATACAACTTCG
59.68
AGGGCTTTTTCAGATCCCCT
58.61
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTCCACGGACCTCCACA
60.61
TCAGCAAACTGTCCAAAGGGA
59.79
148
PCR template sequence:
GCTCTCCACGGACCTCCACACCGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAACAGAAAGAGATTCAGAACATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGGGATCTGAAAAAGCCCTTTGATAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(2), mmu-miR-204-5p(1), mmu-miR-216a-5p(1), mmu-miR-214-3p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
GGAACAGAAAGAGATTCAGAACATGA
siRNA sets
CTGAATCTCTTTCTGTTCC, ATGTTCTGAATCTCTTTCT, TGTTCTGAATCTCTTTCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAP2_495_ORF_1
1.2655
0.40457753
0.997042634
981
ORF sequence:
ATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGGGATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGAAAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGATGGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTGAAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATGGATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAACAGAAAGAGATTCAGAACATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGGGATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGAAAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGATGGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTGAAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATGGATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAACAGAAAGAG
Peptide sequence:
MNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKVKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTAQDEERRQLIQLRDILKSALQVEQKEIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKVKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTAQDEERRQLIQLRDILKSALQVEQKE
IRESfinder result
IRESfinder score
0.420577728
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page