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mmu_circAsap2_1963
Basic information of circular RNA
PanCircBase ID:
mmu_circAsap2_1963
mm39_Chr. Position ID:
chr12|21250880|21304468|+
Gene name:
Asap2
Transcript:
ENSMUST00000064595.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
19
Exon/intron indexes:
5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23
Exon/intron offsets:
0,3080,5423,11929,12268,13482,17095,17308,23425,28359,28494,29943,38675,43806,46408,48422,50259,51861,53335
Genomic length:
53588
Exon/intron sizes in nt:
50,130,86,76,78,9,104,70,88,49,167,134,95,190,86,116,68,114,253
Flanking exon/introns:
chr12:21249619-21250880|chr12:21304468-21306475
Splice length:
1963
Other database IDs:
circAtlas: mmu-Asap2_0047
Human Ortholog IDs:
Mature spliced Sequence:
GATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGAAAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGATGGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTGAAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATGGATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAACAGAAAGAGTCTAGGAGAGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGCATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCAGAAGAGGAAGTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCCAAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCACATGACAGGACGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAACAGCAAGGAAGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACCAAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATCCGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTGCTTGCCAAGAATATCGGGAATGCAGGCTTTAATGAAATTATGGAGTGTTGCCTACCGTCTGAGGACCCAGTCAAGCCCAACCCAGGCAGTGACATGATTGCAAGGAAGGACTACATCACAGCCAAGTACATGGAGAGGAGATATGCACGGAAAAAGCATGCCGACACCGCGGCGAAGCTCCACAGCCTTTGCGAGGCCGTCAAGACCAGAGACATTTTTGGGTTACTCCAAGCTTATGCTGATGGTGTGGACCTGACAGAGAAAATCCCACTGGCCAATGGGCATGAGCCAGATGAGACAGCCCTCCATCTTGCAGTCAGATCTGTGGACCGGACTTCCCTTCACATTGTAGACTTCCTGGTCCAGAACAGTGGGAACCTGGATAAACAGACAGGCAAGGGCAGCACAGCCCTGCACTACTGCTGCCTGACAGACAATGCTGAGTGCCTCAAGCTCCTCCTGCGGGGAAAAGCCTCCATTGAGATAGCCAATGAGTCAGGAGAGACCCCGTTGGACATTGCTAAGCGGCTCAAGCACGAACACTGTGAGGAACTGCTCACTCAGGCCTTGTCGGGGCGGTTTAACTCCCATGTTCACGTCGAGTATGAATGGCGGCTGCTGCACGAGGACCTGGATGAGAGCGACGACGATGTGGACGAGAAGCTACAGCCTAGTCCCAACCGACGGGAGGACCGGCCTGTTAGCTTCTACCAGCTGGGGTCCAGCCAGTTTCAGTCCAATGCTGTGTCTTTGGCCAGAGATACTGCAAACCTCACCAAGGACAAGCAGAGGGGCTTTGGGCCCAGCATCTTGCAGAATGAGACCTATGGAGCCATCCTGAGTGGCAGCCCGCCCTCCTCCCAGTCAATACCCCCCAGCACCACCAGCGCACCCCCGCTCCCACCTCGGAATGTTGGCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCTCCCAGTCAATACCCCC
60.33
TGGCATGCTCCTTTTTCTCCT
59.92
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCCTCCTCCCAGTCAATAC
59.53
TGGCATGCTCCTTTTTCTCCT
59.92
156
PCR template sequence:
ACCTATGGAGCCATCCTGAGTGGCAGCCCGCCCTCCTCCCAGTCAATACCCCCCAGCACCACCAGCGCACCCCCGCTCCCACCTCGGAATGTTGGCAAAGGATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGAAAAAGGAGCATGCCAAGCTCCACGGGATGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-705(2), mmu-miR-199a-5p(1), mmu-miR-375-3p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-31-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-216a-5p(1), mmu-miR-217-5p(1), mmu-miR-214-3p(1), mmu-miR-125a-5p(1), mmu-miR-676-3p(1), mmu-miR-182-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-377-3p(1), mmu-miR-207(1), mmu-miR-670-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
AATGTTGGCAAAGGATCTGAAAAAGC
siRNA sets
CTTTTTCAGATCCTTTGCC,TTTCAGATCCTTTGCCAAC,TTTTTCAGATCCTTTGCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAP2_1963_ORF_1
1.1905
0.4121896
0.999997265
1974
ORF sequence:
ATGTTGGCAAAGGATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGAAAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGATGGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTGAAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATGGATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAACAGAAAGAGTCTAGGAGAGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGCATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCAGAAGAGGAAGTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCCAAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCACATGACAGGACGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAACAGCAAGGAAGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACCAAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATCCGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTGCTTGCCAAGAATATCGGGAATGCAGGCTTTAATGAAATTATGGAGTGTTGCCTACCGTCTGAGGACCCAGTCAAGCCCAACCCAGGCAGTGACATGATTGCAAGGAAGGACTACATCACAGCCAAGTACATGGAGAGGAGATATGCACGGAAAAAGCATGCCGACACCGCGGCGAAGCTCCACAGCCTTTGCGAGGCCGTCAAGACCAGAGACATTTTTGGGTTACTCCAAGCTTATGCTGATGGTGTGGACCTGACAGAGAAAATCCCACTGGCCAATGGGCATGAGCCAGATGAGACAGCCCTCCATCTTGCAGTCAGATCTGTGGACCGGACTTCCCTTCACATTGTAGACTTCCTGGTCCAGAACAGTGGGAACCTGGATAAACAGACAGGCAAGGGCAGCACAGCCCTGCACTACTGCTGCCTGACAGACAATGCTGAGTGCCTCAAGCTCCTCCTGCGGGGAAAAGCCTCCATTGAGATAGCCAATGAGTCAGGAGAGACCCCGTTGGACATTGCTAAGCGGCTCAAGCACGAACACTGTGAGGAACTGCTCACTCAGGCCTTGTCGGGGCGGTTTAACTCCCATGTTCACGTCGAGTATGAATGGCGGCTGCTGCACGAGGACCTGGATGAGAGCGACGACGATGTGGACGAGAAGCTACAGCCTAGTCCCAACCGACGGGAGGACCGGCCTGTTAGCTTCTACCAGCTGGGGTCCAGCCAGTTTCAGTCCAATGCTGTGTCTTTGGCCAGAGATACTGCAAACCTCACCAAGGACAAGCAGAGGGGCTTTGGGCCCAGCATCTTGCAGAATGAGACCTATGGAGCCATCCTGAGTGGCAGCCCGCCCTCCTCCCAGTCAATACCCCCCAGCACCACCAGCGCACCCCCGCTCCCACCTCGGAATGTTGGCAAA
Peptide sequence:
MLAKDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKVKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTAQDEERRQLIQLRDILKSALQVEQKESRRDSQLRQSTAYSLHQPQGNKEHGTERNGNLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPSEDPVKPNPGSDMIARKDYITAKYMERRYARKKHADTAAKLHSLCEAVKTRDIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDESDDDVDEKLQPSPNRREDRPVSFYQLGSSQFQSNAVSLARDTANLTKDKQRGFGPSILQNETYGAILSGSPPSSQSIPPSTTSAPPLPPRNVGK
IRESfinder result
IRESfinder score
0.339525373
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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