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mmu_circAsap1_857
Basic information of circular RNA
PanCircBase ID:
mmu_circAsap1_857
mm39_Chr. Position ID:
chr15|64021454|64063478|-
Gene name:
Asap1
Transcript:
ENSMUST00000177371.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,3224,9276,10616,16854,18094,23079,23612,41878
Genomic length:
42024
Exon/intron sizes in nt:
70,101,123,76,86,130,50,75,146
Flanking exon/introns:
chr15:64009890-64021454|chr15:64063478-64080332
Splice length:
857
Other database IDs:
circAtlas: mmu-Asap1_0052
circBase: hsa_circ_0085601, circAtlas: hsa-ASAP1_0064, CIRCpedia: HSA_CIRCpedia_89692
Human Ortholog IDs:
Mature spliced Sequence:
ATCATGTACAAAATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACCTTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCAGGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGAGATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGAAAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAATGGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACCAAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATGGCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCAAACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGATCCGAAAGAAGTAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTGACTCTCAGAGCCGGCAAGGTGGGTACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGTGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCACGCAACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAAGTGTGGCAGAGACGGA
59.97
TTGACAAAAGCGGTGCCAAG
59.9
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGTGTGGCAGAGACGGAAG
59.97
TTGACAAAAGCGGTGCCAAG
59.9
162
PCR template sequence:
GAAGGGCTTCCTGCTGAAGAAGAGTGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCACGCAACTATCATGTACAAAATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACCTTGGCACCGCTTTTGTCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-216a-5p(1), mmu-miR-25-3p(1), mmu-miR-214-3p(1), mmu-miR-503-5p(1), mmu-miR-705(1), mmu-miR-3105-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCCCACGCAACTATCATGTACAAAA
siRNA sets
TTTTGTACATGATAGTTGC, TTTGTACATGATAGTTGCG, TTGTACATGATAGTTGCGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAP1_857_ORF_1
1.252
0.248400787
0.91775054
603
ORF sequence:
ATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAATGGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACCAAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATGGCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCAAACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGATCCGAAAGAAGTAGGTGGTTTATATGTTGCCAGCAGGGCTAACAGTGACTCTCAGAGCCGGCAAGGTGGGTACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACGGCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGTGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTGTGCGGTCAAGAACGGGATCCTGACCATCTCCCACGCAACTATCATGTACAAAATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAA
Peptide sequence:
MIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKTKKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLDPKEVGGLYVASRANSDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATIMYKMKKTMHKFWISLEVIF
IRESfinder result
IRESfinder score
0.448982844
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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