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mmu_circAsah2_986
Basic information of circular RNA
PanCircBase ID:
mmu_circAsah2_986
mm39_Chr. Position ID:
chr19|32022289|32038805|-
Gene name:
Asah2
Transcript:
ENSMUST00000235213.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
9,8,7,6,5,4,3
Exon/intron offsets:
0,1195,7943,9590,12907,15662,16397
Genomic length:
16516
Exon/intron sizes in nt:
12,81,77,15,01,61,12,90,00,000
Flanking exon/introns:
chr19:32020958-32022289|chr19:32038805-32078949
Splice length:
986
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TAGATTTGGAAACACATTGAGAAATCAGCCTGAGCAACCTGCAAGGCACAAGGCACAAGATTCTGCATGGTTATTTGCTCTCCCAGGAGGTGAACACTTGTTTTGATTAACAGAGTCAGGGTTGAGATGCCCAGTTGTTCCTCATCTTGGCTCAGAAGAAGCACCTAGGAATAAAAGCTCTAAGCTGGTATTAAGTAGAATGGGCTTAAAGTCCACTACAGGAAACAACAGCTAGTGACAGAAATGGCAAAGCGAACCTTCTCCACCTTGGAGGCATTCCTCATTTTCCTTCTGGTAATAATGACAGTCATCACAGTGGCCCTTCTCACCCTCTTGTTTGTTACCAGTGGGACCATTGAAAACCACAAAGATTCAGGAAATCACTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCTTCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATATCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCCCAACGACTGAGGTTGGAGGTCCTGAAGAGACTAGAGAGTAAATATGGCTCTCTGTATCGAAGAGACAATGTTATCCTGAGTGCCATTCACACACACTCTGGCCCAGCAGGGTTTTTCCAATATACACTCTATATACTCGCCAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCATGAAGAGCATTGATATAGCTCACACAAATCTTAAACCAGGCAAAATCTTTATCAACAAAGGAAATGTTGCTAATGTGCAGATCAACCGAAGCCCCTCCTCTTACCTTCTGAATCCACAGTCAGAGAGAGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACCGAAGCCCCTCCTCTTA
60.25
AGTGTTCACCTCCTGGGAGA
59.81
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGATCAACCGAAGCCCCTC
60.46
AGTGTTCACCTCCTGGGAGA
59.81
154
PCR template sequence:
AACCAGGCAAAATCTTTATCAACAAAGGAAATGTTGCTAATGTGCAGATCAACCGAAGCCCCTCCTCTTACCTTCTGAATCCACAGTCAGAGAGAGCAAGTAGATTTGGAAACACATTGAGAAATCAGCCTGAGCAACCTGCAAGGCACAAGGCACAAGATTCTGCATGGTTATTTGCTCTCCCAGGAGGTGAACACTTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-150-5p(1), mmu-miR-322-5p(1), mmu-miR-495-3p(1), mmu-miR-433-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-370-3p(1), mmu-miR-183-5p(1), mmu-miR-466d-3p(1), mmu-miR-350-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGAGAGAGCAAGTAGATTTGGAAAC
siRNA sets
AATCTACTTGCTCTCTCTG, TTTCCAAATCTACTTGCTC, GTTTCCAAATCTACTTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAH2_986_ORF_1
0.6949
0.09030253
0.924065677
849
ORF sequence:
ATGGCAAAGCGAACCTTCTCCACCTTGGAGGCATTCCTCATTTTCCTTCTGGTAATAATGACAGTCATCACAGTGGCCCTTCTCACCCTCTTGTTTGTTACCAGTGGGACCATTGAAAACCACAAAGATTCAGGAAATCACTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCTTCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATATCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCCCAACGACTGAGGTTGGAGGTCCTGAAGAGACTAGAGAGTAAATATGGCTCTCTGTATCGAAGAGACAATGTTATCCTGAGTGCCATTCACACACACTCTGGCCCAGCAGGGTTTTTCCAATATACACTCTATATACTCGCCAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCATGAAGAGCATTGATATAGCTCACACAAATCTTAAACCAGGCAAAATCTTTATCAACAAAGGAAATGTTGCTAATGTGCAGATCAACCGAAGCCCCTCCTCTTACCTTCTGAATCCACAGTCAGAGAGAGCAAGTAGATTTGGAAACACATTGAGAAATCAGCCTGAGCAACCTGCAAGGCACAAGGCACAAGATTCTGCATGGTTATTTGCTCTCCCAGGAGGTGAACACTTGTTTTGA
Peptide sequence:
MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERASRFGNTLRNQPEQPARHKAQDSAWLFALPGGEHLF
IRESfinder result
IRESfinder score
0.664821291
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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