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mmu_circAsah2_311
Basic information of circular RNA
PanCircBase ID:
mmu_circAsah2_311
mm39_Chr. Position ID:
chr19|32030232|32032040|-
Gene name:
Asah2
Transcript:
ENSMUST00000237104.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
7,6
Exon/intron offsets:
0,1647
Genomic length:
1808
Exon/intron sizes in nt:
1,50,161
Flanking exon/introns:
chr19:32023661-32030232|chr19:32032040-32035196
Splice length:
311
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATTCAGGAAATCACTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCTTCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATATCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCCCAACGACTGAGGTTGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCGTGCTTTTATCTTGGCGG
59.9
TGTGTAATTGGAGGGGGCTG
59.67
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCGTGCTTTTATCTTGGCGG
59.9
GTGTAATTGGAGGGGGCTGG
60.4
160
PCR template sequence:
GTTCAGCCGTGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCCCAACGACTGAGGTTGGAGATTCAGGAAATCACTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCTTCATTTCGGAACTTCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-150-5p(1), mmu-miR-466d-3p(1), mmu-miR-350-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTGAGGTTGGAGATTCAGGAAATCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAH2_311_ORF_1
0.5641
0.162923728
0.254536177
342
ORF sequence:
ATGTATGATTTCCCAACGACTGAGGTTGGAGATTCAGGAAATCACTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCTTCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATATCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCCCAACGACTGAGGTTGGAG
Peptide sequence:
MYDFPTTEVGDSGNHWFSTTLGSTTTQPPPITQTPNFPSFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMISQRLRLE
IRESfinder result
IRESfinder score
0.564648313
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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