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mmu_circAsah1_973
Basic information of circular RNA
PanCircBase ID:
mmu_circAsah1_973
mm39_Chr. Position ID:
chr8|41795921|41807954|-
Gene name:
Asah1
Transcript:
ENSMUST00000034000.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,634,845,3222,3411,4046,5203,6665,9009,11129,11942
Genomic length:
12033
Exon/intron sizes in nt:
57,124,132,82,55,145,46,75,79,87,91
Flanking exon/introns:
chr8:41794722-41795921|chr8:41807954-41813289
Splice length:
973
Other database IDs:
circAtlas: hsa-ASAH1_0008
Human Ortholog IDs:
Mature spliced Sequence:
CTATAGAGGACCAGTTCCGTGGCACACCATAAATCTTGATTTACCACCCTACAAAAGATGGCATGAATTATTGGCTCAAAAGGCACCAGCGTTGAGGATTTTAGTGAATTCCATAACGAGTTTAGTGAATACATTTGTGCCAAGTGGAAAACTAATGAAGATGGTGGATCAAAAGCTGCCTGGTATGATTGGCAGCCTTCCTGACCCTTTTGGAGAGGAAATGAGGGGAATTGCAGATGTTACTGGGATTCCTCTAGGAGAGATTATTTCATTCAACATTTTCTATGAATTGTTTACCATGTGTACATCAATCATAACTGAAGATGAGAAAGGTCATTTACTACATGGGAGAAACATGGATTTTGGAATATTTCTTGGGTGGAATATAAATAATAACACTTGGGTTGTCACAGAAGAATTAAAGCCCTTAACAGTGAATTTGGACTTCCAAAGAAACAATAAGACTGTTTTCAAGGCTACAAGTTTTGTTGGATATGTGGGCATGTTGACAGGATTCAAACCAGGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGAAAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTTATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCTATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTTGTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGAATCTCTCCTTTGCTACCATCTATGATGTCCTATCAACAAAACCTGTCCTCAACAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACCGGCCAAGAAGTGTCTA
59.68
TGCCATCTTTTGTAGGGTGGT
59.57
158
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAACACCGGCCAAGAAGTG
60.54
TGCCATCTTTTGTAGGGTGGT
59.57
162
PCR template sequence:
CAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGAATCTCTCCTTTGCTACCATCTATGATGTCCTATCAACAAAACCTGTCCTCAACAAGCTATAGAGGACCAGTTCCGTGGCACACCATAAATCTTGATTTACCACCCTACAAAAGATGGCATGAATTATTGGCTCAAAAGGCACCAGCGTTGAGGATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(1), mmu-miR-216a-5p(1), mmu-miR-138-5p(1), mmu-miR-96-5p(1), mmu-miR-541-5p(1), mmu-miR-1198-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
TGTCCTCAACAAGCTATAGAGGACCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAH1_973_ORF_1
0.7641
0.005493741
0.968594725
1008
ORF sequence:
ATGATGTCCTATCAACAAAACCTGTCCTCAACAAGCTATAGAGGACCAGTTCCGTGGCACACCATAAATCTTGATTTACCACCCTACAAAAGATGGCATGAATTATTGGCTCAAAAGGCACCAGCGTTGAGGATTTTAGTGAATTCCATAACGAGTTTAGTGAATACATTTGTGCCAAGTGGAAAACTAATGAAGATGGTGGATCAAAAGCTGCCTGGTATGATTGGCAGCCTTCCTGACCCTTTTGGAGAGGAAATGAGGGGAATTGCAGATGTTACTGGGATTCCTCTAGGAGAGATTATTTCATTCAACATTTTCTATGAATTGTTTACCATGTGTACATCAATCATAACTGAAGATGAGAAAGGTCATTTACTACATGGGAGAAACATGGATTTTGGAATATTTCTTGGGTGGAATATAAATAATAACACTTGGGTTGTCACAGAAGAATTAAAGCCCTTAACAGTGAATTTGGACTTCCAAAGAAACAATAAGACTGTTTTCAAGGCTACAAGTTTTGTTGGATATGTGGGCATGTTGACAGGATTCAAACCAGGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGAAAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTTATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCTATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTTGTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGAATCTCTCCTTTGCTACCATCTATGATGTCCTATCAACAAAACCTGTCCTCAACAAG
Peptide sequence:
MMSYQQNLSSTSYRGPVPWHTINLDLPPYKRWHELLAQKAPALRILVNSITSLVNTFVPSGKLMKMVDQKLPGMIGSLPDPFGEEMRGIADVTGIPLGEIISFNIFYELFTMCTSIITEDEKGHLLHGRNMDFGIFLGWNINNNTWVVTEELKPLTVNLDFQRNNKTVFKATSFVGYVGMLTGFKPGLFSLSLNERFSINGGYLGILEWMFGRKDAQWVGFITRSVLENTTSYEEAKNTLTKTKIMAPVYFILGGKKSGEGCVITRERKESLDVYELDPKHGRWYVVQTNYDRWKNTLFIDDRRTPAKKCLNHTTQKNLSFATIYDVLSTKPVLNK
IRESfinder result
IRESfinder score
0.622251431
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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