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mmu_circAsah1_393
Basic information of circular RNA
PanCircBase ID:
mmu_circAsah1_393
mm39_Chr. Position ID:
chr8|41796555|41799387|-
Gene name:
Asah1
Transcript:
ENSMUST00000034000.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
12,11,10,9
Exon/intron offsets:
0,211,2588,2777
Genomic length:
2832
Exon/intron sizes in nt:
124,132,82,55
Flanking exon/introns:
chr8:41795978-41796555|chr8:41799387-41799967
Splice length:
393
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGAAAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTTATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCTATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTTGTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGAACACCGGCCAAGAAGT
60.54
CCCACTGGGCATCTTTCCTT
59.96
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGATGACCGCAGAACACCG
60.95
ACTGGGCATCTTTCCTTCCG
60.04
147
PCR template sequence:
GTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTTGTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGGGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-1198-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
CACCACACAGAAGGGACTGTTCAGTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCASAH1_393_ORF_1
0.7231
0.139468532
0.861467143
717
ORF sequence:
ATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGAAAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTTATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCTATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTTGTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAGGGACTGTTCAGTCTTTCACTAAATGAACGTTTCAGTATAAATGGTGGTTATCTGGGTATCCTAGAATGGATGTTCGGAAGGAAAGATGCCCAGTGGGTAGGGTTTATCACTCGATCAGTTCTGGAAAACACCACAAGTTATGAAGAAGCCAAGAACACACTGACCAAGACCAAGATAATGGCGCCAGTATATTTTATCCTGGGAGGCAAGAAGTCTGGAGAGGGTTGTGTGATCACACGGGAAAGAAAAGAGTCTTTGGATGTCTATGAACTTGATCCTAAGCATGGCAGATGGTATGTGGTACAAACCAATTATGACAGGTGGAAAAACACCTTGTTTATTGATGACCGCAGAACACCGGCCAAGAAGTGTCTAAATCACACCACACAGAAG
Peptide sequence:
MFGRKDAQWVGFITRSVLENTTSYEEAKNTLTKTKIMAPVYFILGGKKSGEGCVITRERKESLDVYELDPKHGRWYVVQTNYDRWKNTLFIDDRRTPAKKCLNHTTQKGLFSLSLNERFSINGGYLGILEWMFGRKDAQWVGFITRSVLENTTSYEEAKNTLTKTKIMAPVYFILGGKKSGEGCVITRERKESLDVYELDPKHGRWYVVQTNYDRWKNTLFIDDRRTPAKKCLNHTTQK
IRESfinder result
IRESfinder score
0.348454882
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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