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mmu_circAs3mt_699
Basic information of circular RNA
PanCircBase ID:
mmu_circAs3mt_699
mm39_Chr. Position ID:
chr19|46697985|46713477|+
Gene name:
As3mt
Transcript:
ENSMUST00000003655.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
5,6,7,8,9,10
Exon/intron offsets:
0,2674,5642,10342,10767,15357
Genomic length:
15492
Exon/intron sizes in nt:
1,37,70,82,13,21,43,130
Flanking exon/introns:
chr19:46697557-46697985|chr19:46713477-46728899
Splice length:
699
Other database IDs:
CIRCpedia: HSA_CIRCpedia_374681
Human Ortholog IDs:
Mature spliced Sequence:
GTCGAAGTGGCTAAAACCTATCTTGAACACCACATGGAAAAATTTGGTTTCCAGGCACCCAATGTGACTTTTCTCCACGGCCGCATCGAGAAGTTGGCAGAGGCTGGGATCCAGAGTGAGAGCTATGATATTGTCATATCCAACTGTGTTATCAACCTTGTTCCTGATAAACAACAAGTCCTCCAGGAGGTCTATCGAGTGCTGAAGCACGGCGGGGAGCTCTATTTCAGTGACGTCTATGCCAGCCTTGAAGTGCCAGAAGACATCAAGTCGCACAAAGTTTTATGGGGGGAATGCCTGGGAGGCGCTCTGTACTGGAAGGATCTTGCCATCATTGCCCAAAAGATTGGGTTCTGCCCTCCACGTTTGGTCACTGCCGATATCATTACTGTTGAAAACAAGGAGCTCGAAGGGGTTCTTGGTGACTGTCGCTTTGTGTCTGCCACATTTCGCCTCTTCAAACTCCCTAAGACAGAGCCAGCCGAAAGATGCCGAGTTGTTTACAATGGAGGAATCAAGGGACATGAAAAGGAACTAATTTTCGATGCAAATTTCACATTCAAGGAAGGCGAAGCTGTTGCAGTGGATGAGGAGACGGCAGCTGTCCTGAAGAACTCACGTTTTGCTCCGGATTTTCTCTTCACACCTGTTGACGCCTCGCTGCCAGCTCCCCAGGGCCGTTCTGAGTTAGAGACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCACGTTTTGCTCCGGAT
59.97
TGGGTGCCTGGAAACCAAAT
60.11
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCACGTTTTGCTCCGGAT
59.97
ATTGGGTGCCTGGAAACCAA
60.11
149
PCR template sequence:
AGCTGTCCTGAAGAACTCACGTTTTGCTCCGGATTTTCTCTTCACACCTGTTGACGCCTCGCTGCCAGCTCCCCAGGGCCGTTCTGAGTTAGAGACAAAGGTCGAAGTGGCTAAAACCTATCTTGAACACCACATGGAAAAATTTGGTTTCCAGGCACCCAATGTGACTTTTCTCCACGGCCGCATCGAGAAGTTGGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-34a-5p(1), mmu-miR-31-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-149-5p(1), mmu-miR-382-3p(1), mmu-miR-224-5p(1), mmu-miR-3470b(1), mmu-miR-339-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTAGAGACAAAGGTCGAAGTGGCTA
siRNA sets
TAGCCACTTCGACCTTTGT, AGCCACTTCGACCTTTGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAS3MT_699_ORF_1
1.2393
0.2813896
0.999674445
1365
ORF sequence:
ATGGAAAAATTTGGTTTCCAGGCACCCAATGTGACTTTTCTCCACGGCCGCATCGAGAAGTTGGCAGAGGCTGGGATCCAGAGTGAGAGCTATGATATTGTCATATCCAACTGTGTTATCAACCTTGTTCCTGATAAACAACAAGTCCTCCAGGAGGTCTATCGAGTGCTGAAGCACGGCGGGGAGCTCTATTTCAGTGACGTCTATGCCAGCCTTGAAGTGCCAGAAGACATCAAGTCGCACAAAGTTTTATGGGGGGAATGCCTGGGAGGCGCTCTGTACTGGAAGGATCTTGCCATCATTGCCCAAAAGATTGGGTTCTGCCCTCCACGTTTGGTCACTGCCGATATCATTACTGTTGAAAACAAGGAGCTCGAAGGGGTTCTTGGTGACTGTCGCTTTGTGTCTGCCACATTTCGCCTCTTCAAACTCCCTAAGACAGAGCCAGCCGAAAGATGCCGAGTTGTTTACAATGGAGGAATCAAGGGACATGAAAAGGAACTAATTTTCGATGCAAATTTCACATTCAAGGAAGGCGAAGCTGTTGCAGTGGATGAGGAGACGGCAGCTGTCCTGAAGAACTCACGTTTTGCTCCGGATTTTCTCTTCACACCTGTTGACGCCTCGCTGCCAGCTCCCCAGGGCCGTTCTGAGTTAGAGACAAAGGTCGAAGTGGCTAAAACCTATCTTGAACACCACATGGAAAAATTTGGTTTCCAGGCACCCAATGTGACTTTTCTCCACGGCCGCATCGAGAAGTTGGCAGAGGCTGGGATCCAGAGTGAGAGCTATGATATTGTCATATCCAACTGTGTTATCAACCTTGTTCCTGATAAACAACAAGTCCTCCAGGAGGTCTATCGAGTGCTGAAGCACGGCGGGGAGCTCTATTTCAGTGACGTCTATGCCAGCCTTGAAGTGCCAGAAGACATCAAGTCGCACAAAGTTTTATGGGGGGAATGCCTGGGAGGCGCTCTGTACTGGAAGGATCTTGCCATCATTGCCCAAAAGATTGGGTTCTGCCCTCCACGTTTGGTCACTGCCGATATCATTACTGTTGAAAACAAGGAGCTCGAAGGGGTTCTTGGTGACTGTCGCTTTGTGTCTGCCACATTTCGCCTCTTCAAACTCCCTAAGACAGAGCCAGCCGAAAGATGCCGAGTTGTTTACAATGGAGGAATCAAGGGACATGAAAAGGAACTAATTTTCGATGCAAATTTCACATTCAAGGAAGGCGAAGCTGTTGCAGTGGATGAGGAGACGGCAGCTGTCCTGAAGAACTCACGTTTTGCTCCGGATTTTCTCTTCACACCTGTTGACGCCTCGCTGCCAGCTCCCCAGGGCCGTTCTGAGTTAGAGACAAAG
Peptide sequence:
MEKFGFQAPNVTFLHGRIEKLAEAGIQSESYDIVISNCVINLVPDKQQVLQEVYRVLKHGGELYFSDVYASLEVPEDIKSHKVLWGECLGGALYWKDLAIIAQKIGFCPPRLVTADIITVENKELEGVLGDCRFVSATFRLFKLPKTEPAERCRVVYNGGIKGHEKELIFDANFTFKEGEAVAVDEETAAVLKNSRFAPDFLFTPVDASLPAPQGRSELETKVEVAKTYLEHHMEKFGFQAPNVTFLHGRIEKLAEAGIQSESYDIVISNCVINLVPDKQQVLQEVYRVLKHGGELYFSDVYASLEVPEDIKSHKVLWGECLGGALYWKDLAIIAQKIGFCPPRLVTADIITVENKELEGVLGDCRFVSATFRLFKLPKTEPAERCRVVYNGGIKGHEKELIFDANFTFKEGEAVAVDEETAAVLKNSRFAPDFLFTPVDASLPAPQGRSELETK
IRESfinder result
IRESfinder score
0.573692493
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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