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mmu_circAs3mt_278
Basic information of circular RNA
PanCircBase ID:
mmu_circAs3mt_278
mm39_Chr. Position ID:
chr19|46708752|46713477|+
Gene name:
As3mt
Transcript:
ENSMUST00000003655.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
9,10
Exon/intron offsets:
0,4590
Genomic length:
4725
Exon/intron sizes in nt:
1,43,135
Flanking exon/introns:
chr19:46708459-46708752|chr19:46713477-46728899
Splice length:
278
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGACTGTCGCTTTGTGTCTGCCACATTTCGCCTCTTCAAACTCCCTAAGACAGAGCCAGCCGAAAGATGCCGAGTTGTTTACAATGGAGGAATCAAGGGACATGAAAAGGAACTAATTTTCGATGCAAATTTCACATTCAAGGAAGGCGAAGCTGTTGCAGTGGATGAGGAGACGGCAGCTGTCCTGAAGAACTCACGTTTTGCTCCGGATTTTCTCTTCACACCTGTTGACGCCTCGCTGCCAGCTCCCCAGGGCCGTTCTGAGTTAGAGACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTCACGTTTTGCTCCGGAT
59.97
ATCTTTCGGCTGGCTCTGTC
60.11
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACTCACGTTTTGCTCCGGA
60.18
ATCTTTCGGCTGGCTCTGTC
60.11
156
PCR template sequence:
AGCTGTCCTGAAGAACTCACGTTTTGCTCCGGATTTTCTCTTCACACCTGTTGACGCCTCGCTGCCAGCTCCCCAGGGCCGTTCTGAGTTAGAGACAAAGGTGACTGTCGCTTTGTGTCTGCCACATTTCGCCTCTTCAAACTCCCTAAGACAGAGCCAGCCGAAAGATGCCGAGTTGTTTACAATGGAGGAATCAAGGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-149-5p(1), mmu-miR-382-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTAGAGACAAAGGTGACTGTCGCTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.498389016
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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