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mmu_circArhgef7_291
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgef7_291
mm39_Chr. Position ID:
chr8|11835712|11841544|+
Gene name:
Arhgef7
Transcript:
ENSMUST00000110909.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
5,6
Exon/intron offsets:
0,5743
Genomic length:
5832
Exon/intron sizes in nt:
202,89
Flanking exon/introns:
chr8:11832655-11835712|chr8:11841544-11850459
Splice length:
291
Other database IDs:
circBase: mmu_circ_0014613, CIRCpedia: MMU_CIRCpedia_4379, circAtlas: mmu-Arhgef7_0015
circBase: hsa_circ_0099900, circAtlas: hsa-ARHGEF7_0017, CIRCpedia: HSA_CIRCpedia_10091
Human Ortholog IDs:
Mature spliced Sequence:
GACATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATGAACTCTCCTTCTCAAAGGGTGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCACACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTGTCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATTACAACGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCCAAGTGAGAAGCCCG
60.3
GGCTCGTACTACCAGTTGGC
60.46
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCCAAGTGAGAAGCCCG
60.3
GCTCGTACTACCAGTTGGCT
59.47
140
PCR template sequence:
CAAGCCAAGTGAGAAGCCCGTGTCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATTACAACGTGGACATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATGAACTCTCCTTCTCAAAGGGTGATGTCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTATTACAACGTGGACATGACTGATA
siRNA sets
TATCAGTCATGTCCACGTT,ATCAGTCATGTCCACGTTG,TCAGTCATGTCCACGTTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGEF7_291_ORF_1
0.915
0.441085394
0.918825006
579
ORF sequence:
ATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATGAACTCTCCTTCTCAAAGGGTGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCACACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTGTCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATTACAACGTGGACATGACTGATAACACCAACAGCCAACTGGTAGTACGAGCCAAGTTTAACTTCCAGCAGACCAATGAAGATGAACTCTCCTTCTCAAAGGGTGATGTCATCCATGTCACACGAGTGGAGGAAGGAGGCTGGTGGGAGGGCACACACAATGGCAGGACCGGCTGGTTCCCCAGCAACTACGTTCGAGAGATCAAGCCAAGTGAGAAGCCCGTGTCACCCAAATCAGGGACCTTGAAGAGCCCTCCCAAAGGGTTCGATACGACTGCCATCAACAAGAGCTATTACAACGTG
Peptide sequence:
MTDNTNSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTGWFPSNYVREIKPSEKPVSPKSGTLKSPPKGFDTTAINKSYYNVDMTDNTNSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTHNGRTGWFPSNYVREIKPSEKPVSPKSGTLKSPPKGFDTTAINKSYYNV
IRESfinder result
IRESfinder score
0.243998016
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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