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mmu_circArhgap44_468
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap44_468
mm39_Chr. Position ID:
chr11|64912766|64917710|-
Gene name:
Arhgap44
Transcript:
ENSMUST00000047463.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
16,15,14,13
Exon/intron offsets:
0,1185,2250,4863
Genomic length:
4944
Exon/intron sizes in nt:
181,92,114,81
Flanking exon/introns:
chr11:64902924-64912766|chr11:64917710-64922476
Splice length:
468
Other database IDs:
circAtlas: mmu-Arhgap44_0017
Human Ortholog IDs:
Mature spliced Sequence:
TATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCAACCATAACAACATCAAATACTTGATCAAATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGATGACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACCGAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGTTCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGCCAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGGCCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGACATGGACCCTGCTGAC
60.39
TGGGCAACTTCTCACAAGCA
60.11
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACATGGACCCTGCTGACC
60.08
TGGGCAACTTCTCACAAGCA
60.11
157
PCR template sequence:
CAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGGCCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGGTATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCAACCATAACAACATCAAATACTTGATCAAATTTTTA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-26b-5p(1), mmu-miR-697(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACAAGAAGGATGGTATCCAGGAGCAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP44_468_ORF_1
1.2973
0.423549436
0.990617878
801
ORF sequence:
ATGACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACCGAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGTTCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGCCAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGGCCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGATGGTATCCAGGAGCAAGACAAGAGACTTCAGGCTCTCTGGAATGCTTGTGAGAAGTTGCCCAAGGCCAACCATAACAACATCAAATACTTGATCAAATTTTTATCGAAACTGTCTGAATATCAGGATGTAAACAAGATGACTCCCAGTAACATGGCAATTGTCCTGGGACCCAATCTCCTTTGGCCACAGTCAGAGGGGAACATCACCGAGATGATGACCACAGTTTCCCTGCAGATCGTTGGCATCATCGAGCCCATTATCCAGCACGCAGACTGGTTCTTCCCTGGAGAGATAGAGTTCAACCTCACAGGCAGTTACGGGAGTCCAGTCCACGTGAACCACAATGCCAACTACAGCTCAATGCCCTCCCCAGACATGGACCCTGCTGACCGGCGCCAGCCAGAGCAGGCCCGCCGGCCCCTCAGTGTTGCCACAGACAACATGATGCTGGAGTTTTACAAGAAGGAT
Peptide sequence:
MTPSNMAIVLGPNLLWPQSEGNITEMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNLTGSYGSPVHVNHNANYSSMPSPDMDPADRRQPEQARRPLSVATDNMMLEFYKKDGIQEQDKRLQALWNACEKLPKANHNNIKYLIKFLSKLSEYQDVNKMTPSNMAIVLGPNLLWPQSEGNITEMMTTVSLQIVGIIEPIIQHADWFFPGEIEFNLTGSYGSPVHVNHNANYSSMPSPDMDPADRRQPEQARRPLSVATDNMMLEFYKKD
IRESfinder result
IRESfinder score
0.392213019
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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