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mmu_circArhgap32_679
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap32_679
mm39_Chr. Position ID:
chr9|32157742|32162083|+
Gene name:
Arhgap32
Transcript:
ENSMUST00000174641.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
14,15,16,17,18
Exon/intron offsets:
0,661,1039,1933,4195
Genomic length:
4341
Exon/intron sizes in nt:
83,108,146,196,146
Flanking exon/introns:
chr9:32157358-32157742|chr9:32162083-32165362
Splice length:
679
Other database IDs:
CIRCpedia: MMU_CIRCpedia_16463, circAtlas: mmu-Arhgap32_0022
Human Ortholog IDs:
Mature spliced Sequence:
GATGCTGTTTCAGCAGCAACGGATGAAGAACGGTTGATAAAAATCCATGATGTCATCCAGCAACTCCCCCCACCACACTATAGAACCCTGGAGTTCCTGATGAGACACCTGTCTCTTCTAGCAGACTATTGCTCCATCACAAATATGCATGCGAAAAACCTTGCCATTGTGTGGGCACCAAACCTGCTGAGATCAAAGCAGATAGAGTCAGCTTGCTTCAGTGGGACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAATTCATTCTCAATCATGTGGATGTGCTCTTCAGTGGCAAAATCAGCGCTGTCATGCAGGAAGGAGCAGCTTCTCTGTCAAGGCCCAAGTCCCTGCTGGTCTCGTCTCCATCTACCAAGCTGCTGACATTGGAGGAAGCCCAGGCAAGGACGCAGGCTCAGGTCAGTTCTCCTATTGTGACGGAAAATAAGTACATCGAAGTCGGAGAAGGGCCTGCTGCGCTGCAAGGGAAGTTTCACACCGTCATTGAGTTCCCACTTGAAAGAAAGAGGCCTCAGAATAAGATGAAAAAATCTCCCGTTGGCAGCTGGCGTTCCTTTTTCAATTTGGGGAAGTCCTCTTCTGTCTCCAAACGCAAGTTGCAGCGTAATGAAAGTGAGCCCTCAGAAATGAAAGCCATGGCTTTGAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCTCTTCTGTCTCCAAACGC
60
GGGGAGTTGCTGGATGACAT
59.74
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGGAAGTCCTCTTCTGTCT
59.85
GGGGAGTTGCTGGATGACAT
59.74
153
PCR template sequence:
CGTTCCTTTTTCAATTTGGGGAAGTCCTCTTCTGTCTCCAAACGCAAGTTGCAGCGTAATGAAAGTGAGCCCTCAGAAATGAAAGCCATGGCTTTGAAAGGATGCTGTTTCAGCAGCAACGGATGAAGAACGGTTGATAAAAATCCATGATGTCATCCAGCAACTCCCCCCACCACACTATAGAACCCTGGAGTTCCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(2), mmu-miR-135a-5p(1), mmu-miR-146a-5p(1), mmu-miR-22-3p(1), mmu-miR-377-3p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATGGCTTTGAAAGGATGCTGTTTCAG
siRNA sets
CAGCATCCTTTCAAAGCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP32_679_ORF_1
0.9508
0.171971886
0.827712835
606
ORF sequence:
ATGAGACACCTGTCTCTTCTAGCAGACTATTGCTCCATCACAAATATGCATGCGAAAAACCTTGCCATTGTGTGGGCACCAAACCTGCTGAGATCAAAGCAGATAGAGTCAGCTTGCTTCAGTGGGACAGCAGCTTTCATGGAAGTGAGGATTCAGTCTGTGGTTGTTGAATTCATTCTCAATCATGTGGATGTGCTCTTCAGTGGCAAAATCAGCGCTGTCATGCAGGAAGGAGCAGCTTCTCTGTCAAGGCCCAAGTCCCTGCTGGTCTCGTCTCCATCTACCAAGCTGCTGACATTGGAGGAAGCCCAGGCAAGGACGCAGGCTCAGGTCAGTTCTCCTATTGTGACGGAAAATAAGTACATCGAAGTCGGAGAAGGGCCTGCTGCGCTGCAAGGGAAGTTTCACACCGTCATTGAGTTCCCACTTGAAAGAAAGAGGCCTCAGAATAAGATGAAAAAATCTCCCGTTGGCAGCTGGCGTTCCTTTTTCAATTTGGGGAAGTCCTCTTCTGTCTCCAAACGCAAGTTGCAGCGTAATGAAAGTGAGCCCTCAGAAATGAAAGCCATGGCTTTGAAAGGATGCTGTTTCAGCAGCAACGGATGA
Peptide sequence:
MRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSGKISAVMQEGAASLSRPKSLLVSSPSTKLLTLEEAQARTQAQVSSPIVTENKYIEVGEGPAALQGKFHTVIEFPLERKRPQNKMKKSPVGSWRSFFNLGKSSSVSKRKLQRNESEPSEMKAMALKGCCFSSNG
IRESfinder result
IRESfinder score
0.520892934
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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