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mmu_circArhgap26_1384
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap26_1384
mm39_Chr. Position ID:
chr18|39230682|39379977|+
Gene name:
Arhgap26
Transcript:
ENSMUST00000097593.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
16
Exon/intron indexes:
2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17
Exon/intron offsets:
0,1942,4539,12825,14160,22489,24144,29203,34426,52410,107654,129738,132261,145976,147653,149189
Genomic length:
149295
Exon/intron sizes in nt:
96,62,72,102,111,105,130,101,95,79,37,66,75,88,59,106
Flanking exon/introns:
chr18:39126682-39230682|chr18:39379977-39419579
Splice length:
1384
Other database IDs:
CIRCpedia: MMU_CIRCpedia_225467, circAtlas: mmu-Arhgap26_0025
circAtlas: hsa-ARHGAP26_0032, CIRCpedia: HSA_CIRCpedia_158788
Human Ortholog IDs:
Mature spliced Sequence:
ATTTGTCTTCGGCAAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAGTGTATAGGAGATGCCGAAACAGATGATGAGATGTGTATAGCGAGATCTTTGCAAGAATTTGCTGCTGTTCTCAGGAATCTTGAAGACGAGCGGAGTCGAATGATCGAGAATGCCAGCGAGGTGCTCATCACACCCTTGGAGAAGTTTCGAAAGGAGCAGATTGGGGCCGCCAGGGAAGCCAAAAAGAAGTATGACAAAGAGACCGAGAAGTACTGCGGCACCTTAGAAAAACATTTGAATTTGTCTTCCAAAAAGAAGGAATCCCAGCTACAGGAGGCAGACAGCCAAGTGGACCTGGTTCGGCAACATTTCTACGAAGTATCCCTGGAGTATGTCTTTAAGGTGCAGGAAGTGCAAGAGAGAAAGATGTTTGAGTTTGTAGAGCCTCTGCTGGCCTTCCTGCAAGGACTCTTCACTTTCTATCACCATGGTTATGAGCTGGCCAAGGATTTTGGTGACTTTAAGACACAGTTGACCATCAGCATACAAAATACAAGAAACCGCTTTGAAGGAACCAGGTCTGAGGTGGAATCGCTGATGAAGAAGATGAAGGAAAACCCCCTGGAGCACAAGACCATCAGCCCGTACACCATGGAAGGGTACCTGTACGTGCAGGAGAAGCGTCACTTTGGAACTTCCTGGGTGAAGCACTACTGTACCTACCAACGGGATTCCAAACAAATCACCATGGTTCCATTCGACCAAAAGTCAGGAGGAAAGGGGGGAGAAGACGAGTCCGTTACCCTCAAATCCTGCACACGGCGAAAGACAGACTCCATCGAGAAGCGATTCTGCTTTGACGTGGAAGCAGTAGACAGGCCAGGGGTTATCACCATGCAGGCGTTGTCGGAAGAGGACCGTAGGCTCTGGATGGAAGCCATGGATGGCCGGGAACCTGTGTACAACTCCAACAGAGACAGCCAGAGCGAAGGGACCGCACAATTGGACAGTATCGGCTTCAGCATAATAAGGAAATGCATCCATGCGGTGGAGACCAGAGGGATCAATGAGCAGGGGCTCTACCGAATCGTGGGGGTCAACTCCAGGGTGCAGAAGTTGCTGAGCGTCCTGATGGACCCCAAAGCAGCTTCTGAGACAGAAACTGATATCTGTGCCGAATGGGAGATAAAGACCGTCACCAGTGCTCTGAAGACCTACCTGAGAATGCTCCCAGGACCCCTCATGATGTACCAGTTTCAAAGAAGTTTCATCAAAGCAGCAAAGCTGGAAAACCAAGAGACTCGAGTGTCTGAGATCCACAGTCTTGTCCATCGGCTCCCGGAGAAGAATCGGCAGATGTTGCAGCTGCTCATGAACCACCTGGCCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACAGTCTTGTCCATCGGCT
60.04
TCATCTGTTTCGGCATCTCCT
59.17
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGTCTTGTCCATCGGCTC
59.75
TCATCTGTTTCGGCATCTCCT
59.17
152
PCR template sequence:
AAAACCAAGAGACTCGAGTGTCTGAGATCCACAGTCTTGTCCATCGGCTCCCGGAGAAGAATCGGCAGATGTTGCAGCTGCTCATGAACCACCTGGCCAAATTTGTCTTCGGCAAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAGTGTATAGGAGATGCCGAAACAGATGATGAGATGTGTATAGCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
13
Interacting miRNAs (#binding sites)
mmu-miR-207(2), mmu-miR-7b-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-18a-5p(1), mmu-miR-7a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-199b-3p(1), mmu-miR-199a-3p(1), mmu-miR-224-5p(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1), mmu-miR-5101(1)
siRNAs for circular RNA silencing
Junction sequence
ACCACCTGGCCAAATTTGTCTTCGGC
siRNA sets
AAGACAAATTTGGCCAGGT, ACAAATTTGGCCAGGTGGT, AGACAAATTTGGCCAGGTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP26_1384_ORF_1
1.2361
0.382477994
0.999734014
1341
ORF sequence:
ATGTGTATAGCGAGATCTTTGCAAGAATTTGCTGCTGTTCTCAGGAATCTTGAAGACGAGCGGAGTCGAATGATCGAGAATGCCAGCGAGGTGCTCATCACACCCTTGGAGAAGTTTCGAAAGGAGCAGATTGGGGCCGCCAGGGAAGCCAAAAAGAAGTATGACAAAGAGACCGAGAAGTACTGCGGCACCTTAGAAAAACATTTGAATTTGTCTTCCAAAAAGAAGGAATCCCAGCTACAGGAGGCAGACAGCCAAGTGGACCTGGTTCGGCAACATTTCTACGAAGTATCCCTGGAGTATGTCTTTAAGGTGCAGGAAGTGCAAGAGAGAAAGATGTTTGAGTTTGTAGAGCCTCTGCTGGCCTTCCTGCAAGGACTCTTCACTTTCTATCACCATGGTTATGAGCTGGCCAAGGATTTTGGTGACTTTAAGACACAGTTGACCATCAGCATACAAAATACAAGAAACCGCTTTGAAGGAACCAGGTCTGAGGTGGAATCGCTGATGAAGAAGATGAAGGAAAACCCCCTGGAGCACAAGACCATCAGCCCGTACACCATGGAAGGGTACCTGTACGTGCAGGAGAAGCGTCACTTTGGAACTTCCTGGGTGAAGCACTACTGTACCTACCAACGGGATTCCAAACAAATCACCATGGTTCCATTCGACCAAAAGTCAGGAGGAAAGGGGGGAGAAGACGAGTCCGTTACCCTCAAATCCTGCACACGGCGAAAGACAGACTCCATCGAGAAGCGATTCTGCTTTGACGTGGAAGCAGTAGACAGGCCAGGGGTTATCACCATGCAGGCGTTGTCGGAAGAGGACCGTAGGCTCTGGATGGAAGCCATGGATGGCCGGGAACCTGTGTACAACTCCAACAGAGACAGCCAGAGCGAAGGGACCGCACAATTGGACAGTATCGGCTTCAGCATAATAAGGAAATGCATCCATGCGGTGGAGACCAGAGGGATCAATGAGCAGGGGCTCTACCGAATCGTGGGGGTCAACTCCAGGGTGCAGAAGTTGCTGAGCGTCCTGATGGACCCCAAAGCAGCTTCTGAGACAGAAACTGATATCTGTGCCGAATGGGAGATAAAGACCGTCACCAGTGCTCTGAAGACCTACCTGAGAATGCTCCCAGGACCCCTCATGATGTACCAGTTTCAAAGAAGTTTCATCAAAGCAGCAAAGCTGGAAAACCAAGAGACTCGAGTGTCTGAGATCCACAGTCTTGTCCATCGGCTCCCGGAGAAGAATCGGCAGATGTTGCAGCTGCTCATGAACCACCTGGCCAAATTTGTCTTCGGCAAAGCGGAAGTTTGCAGATTCCTTAAATGA
Peptide sequence:
MCIARSLQEFAAVLRNLEDERSRMIENASEVLITPLEKFRKEQIGAAREAKKKYDKETEKYCGTLEKHLNLSSKKKESQLQEADSQVDLVRQHFYEVSLEYVFKVQEVQERKMFEFVEPLLAFLQGLFTFYHHGYELAKDFGDFKTQLTISIQNTRNRFEGTRSEVESLMKKMKENPLEHKTISPYTMEGYLYVQEKRHFGTSWVKHYCTYQRDSKQITMVPFDQKSGGKGGEDESVTLKSCTRRKTDSIEKRFCFDVEAVDRPGVITMQALSEEDRRLWMEAMDGREPVYNSNRDSQSEGTAQLDSIGFSIIRKCIHAVETRGINEQGLYRIVGVNSRVQKLLSVLMDPKAASETETDICAEWEIKTVTSALKTYLRMLPGPLMMYQFQRSFIKAAKLENQETRVSEIHSLVHRLPEKNRQMLQLLMNHLAKFVFGKAEVCRFLK
IRESfinder result
IRESfinder score
0.355861007
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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