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mmu_circArhgap21_3638

Basic information of circular RNA
PanCircBase ID:

mmu_circArhgap21_3638

mm39_Chr. Position ID:

chr2|20865781|20972531|-

Gene name:

Arhgap21

Transcript:

ENSMUST00000114594.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

15

Exon/intron indexes:

16,15,14,13,12,11,10,9,8,7,6,5,4,3,2

Exon/intron offsets:

0,215,2109,4294,6831,10421,10639,18990,22229,26135,28904,31114,51306,53661,106377

Genomic length:

106750

Exon/intron sizes in nt:

13,76,52,85,12,63,71,00,00,00,00,00,00,00,00,00,00,00,000

Flanking exon/introns:

chr2:20865257-20865781|chr2:20972531-20973294

Splice length:

3638

Other database IDs:

Human Ortholog IDs:
Mature spliced Sequence:

GAAGGCTCTCCGAAAGCTGATTTTTCTCTGGCCTCCGAGTAACACCGCCTGGAAGCGGACCTCTTCTCTCTGCACTTGGCTTCCACCCTACCTGCCAAGGAAACCTGTCGCCCTTGAACTTTAAGACTGGAGAAATGAAGAGGGCTAAGTCTGTTGAAGAAGGTATTTGAAGGGGCTTCCTGCAAATCGTTTTTTTGACCAGTCCTTCATCGCCTCAGAATCTGCTGTGGAATTGCTTCCCGTTTGGTGTGTGGTGGCACTTCTCCAATGTGGACTCCAAAGGATTTGTCCCTTTACAATTTGAAATGAAATGATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCCATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGACACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAGGAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCCGGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAGACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGGATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

AGCCAAAGACTCAAAGCCCA

59.81

CTTGGCAGGTAGGGTGGAAG

60.04

162

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

AGCCAAAGACTCAAAGCCCA

59.81

TGGAAGCCAAGTGCAGAGAG

59.96

148

PCR template sequence:

CTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGGAAGGCTCTCCGAAAGCTGATTTTTCTCTGGCCTCCGAGTAACACCGCCTGGAAGCGGACCTCTTCTCTCTGCACTTGGCTTCCACCCTACCTGCCAAGG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

18

Interacting miRNAs (#binding sites)

mmu-miR-136-5p(3), mmu-miR-138-5p(2), mmu-miR-200b-3p(1), mmu-miR-150-5p(1), mmu-miR-34c-5p(1), mmu-miR-200c-3p(1), mmu-miR-204-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-143-3p(1), mmu-miR-382-3p(1), mmu-miR-127-5p(1), mmu-miR-96-5p(1), mmu-miR-499-5p(1), mmu-miR-325-3p(1), mmu-miR-342-3p(1), mmu-miR-129-5p(1), mmu-miR-146b-5p(1)

siRNAs for circular RNA silencing
Junction sequence

CTTCTCAGTAAAGGAAGGCTCTCCGA

siRNA sets

AGAGCCTTCCTTTACTGAG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCARHGAP21_3638_ORF_1

0.8051

0.158436332

0.999999999

3348

ORF sequence:

ATGATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCCATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGACACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAGGAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCCGGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAGACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGGATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGGAAGGCTCTCCGAAAGCTGA

Peptide sequence:

MMATHWTGLPEEDGDKLKACGVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKGRLSES

IRESfinder result

IRESfinder score

0.55313479

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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