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mmu_circArhgap21_3638
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap21_3638
mm39_Chr. Position ID:
chr2|20865781|20972531|-
Gene name:
Arhgap21
Transcript:
ENSMUST00000114594.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
15
Exon/intron indexes:
16,15,14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,215,2109,4294,6831,10421,10639,18990,22229,26135,28904,31114,51306,53661,106377
Genomic length:
106750
Exon/intron sizes in nt:
13,76,52,85,12,63,71,00,00,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:20865257-20865781|chr2:20972531-20973294
Splice length:
3638
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAAGGCTCTCCGAAAGCTGATTTTTCTCTGGCCTCCGAGTAACACCGCCTGGAAGCGGACCTCTTCTCTCTGCACTTGGCTTCCACCCTACCTGCCAAGGAAACCTGTCGCCCTTGAACTTTAAGACTGGAGAAATGAAGAGGGCTAAGTCTGTTGAAGAAGGTATTTGAAGGGGCTTCCTGCAAATCGTTTTTTTGACCAGTCCTTCATCGCCTCAGAATCTGCTGTGGAATTGCTTCCCGTTTGGTGTGTGGTGGCACTTCTCCAATGTGGACTCCAAAGGATTTGTCCCTTTACAATTTGAAATGAAATGATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCCATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGACACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAGGAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCCGGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAGACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGGATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCAAAGACTCAAAGCCCA
59.81
CTTGGCAGGTAGGGTGGAAG
60.04
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGCCAAAGACTCAAAGCCCA
59.81
TGGAAGCCAAGTGCAGAGAG
59.96
148
PCR template sequence:
CTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGGAAGGCTCTCCGAAAGCTGATTTTTCTCTGGCCTCCGAGTAACACCGCCTGGAAGCGGACCTCTTCTCTCTGCACTTGGCTTCCACCCTACCTGCCAAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
18
Interacting miRNAs (#binding sites)
mmu-miR-136-5p(3), mmu-miR-138-5p(2), mmu-miR-200b-3p(1), mmu-miR-150-5p(1), mmu-miR-34c-5p(1), mmu-miR-200c-3p(1), mmu-miR-204-5p(1), mmu-miR-196a-5p(1), mmu-miR-320-3p(1), mmu-miR-143-3p(1), mmu-miR-382-3p(1), mmu-miR-127-5p(1), mmu-miR-96-5p(1), mmu-miR-499-5p(1), mmu-miR-325-3p(1), mmu-miR-342-3p(1), mmu-miR-129-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTCTCAGTAAAGGAAGGCTCTCCGA
siRNA sets
AGAGCCTTCCTTTACTGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP21_3638_ORF_1
0.8051
0.158436332
0.999999999
3348
ORF sequence:
ATGATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCCATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGACACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAGGAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCCGGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAGACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGGATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGGAAGGCTCTCCGAAAGCTGA
Peptide sequence:
MMATHWTGLPEEDGDKLKACGVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLRHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGKTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKGRLSES
IRESfinder result
IRESfinder score
0.55313479
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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