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mmu_circArhgap10_476
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap10_476
mm39_Chr. Position ID:
chr8|78037359|78078301|-
Gene name:
Arhgap10
Transcript:
ENSMUST00000076316.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
19,18,17,16
Exon/intron offsets:
0,33909,35541,40883
Genomic length:
40942
Exon/intron sizes in nt:
151,160,106,59
Flanking exon/introns:
chr8:78003936-78037359|chr8:78078301-78085175
Splice length:
476
Other database IDs:
circBase: mmu_circ_0014980, CIRCpedia: MMU_CIRCpedia_29607, circAtlas: mmu-Arhgap10_0004
circAtlas: hsa-ARHGAP10_0011
Human Ortholog IDs:
Mature spliced Sequence:
GAGTCTTCCAGAGCCTCTCATGACCTATGAGTTACACAGAGACTTCATTGTTCCAGCCAAGAGCGGCAGCCCGGAATCCCGAGTTAATGCCATCCACTTCTTGGTACACAAACTGCCAGAGAAGAATAAGGAGATGTTGGATATCTTGGTGAAGCATCTAACCAACGTGTCAAGTCACTCGAAGCAGAATCTAATGACAGTGGCGAATTTAGGGGTGGTGTTTGGACCAACTCTGATGAGACCACAGGAAGAGACTGTTGCCGCCATCATGGATTTGAAGTTTCAGAATATCGTTGTAGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGAACCTCGCCTGATACCACATTTGCTGAGCCCACATGCCTGTCAGCATCACCCCCAAACGCTCCACCACGACAGTCCAAGAGACAAGGGCAGAGGACGAAGAGACCAGTGGCCGTGTACAACCTTTGTCTTGAGCTGGAGGAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCACCACGACAGTCCAAGAG
60.32
CGCTCTTGGCTGGAACAATG
59.83
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCACCACGACAGTCCAAG
60.32
CGCTCTTGGCTGGAACAATG
59.83
149
PCR template sequence:
GCATCACCCCCAAACGCTCCACCACGACAGTCCAAGAGACAAGGGCAGAGGACGAAGAGACCAGTGGCCGTGTACAACCTTTGTCTTGAGCTGGAGGAGGGAGTCTTCCAGAGCCTCTCATGACCTATGAGTTACACAGAGACTTCATTGTTCCAGCCAAGAGCGGCAGCCCGGAATCCCGAGTTAATGCCATCCACTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-132-3p(1), mmu-miR-212-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGCTGGAGGAGGGAGTCTTCCAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP10_476_ORF_1
1.0162
0.220466477
0.729014675
480
ORF sequence:
ATGACCTATGAGTTACACAGAGACTTCATTGTTCCAGCCAAGAGCGGCAGCCCGGAATCCCGAGTTAATGCCATCCACTTCTTGGTACACAAACTGCCAGAGAAGAATAAGGAGATGTTGGATATCTTGGTGAAGCATCTAACCAACGTGTCAAGTCACTCGAAGCAGAATCTAATGACAGTGGCGAATTTAGGGGTGGTGTTTGGACCAACTCTGATGAGACCACAGGAAGAGACTGTTGCCGCCATCATGGATTTGAAGTTTCAGAATATCGTTGTAGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGAACCTCGCCTGATACCACATTTGCTGAGCCCACATGCCTGTCAGCATCACCCCCAAACGCTCCACCACGACAGTCCAAGAGACAAGGGCAGAGGACGAAGAGACCAGTGGCCGTGTACAACCTTTGTCTTGAGCTGGAGGAGGGAGTCTTCCAGAGCCTCTCATGA
Peptide sequence:
MTYELHRDFIVPAKSGSPESRVNAIHFLVHKLPEKNKEMLDILVKHLTNVSSHSKQNLMTVANLGVVFGPTLMRPQEETVAAIMDLKFQNIVVEILIENHEKIFRTSPDTTFAEPTCLSASPPNAPPRQSKRQGQRTKRPVAVYNLCLELEEGVFQSLS
IRESfinder result
IRESfinder score
0.484197598
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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