top of page
mmu_circAqr_1672
Basic information of circular RNA
PanCircBase ID:
mmu_circAqr_1672
mm39_Chr. Position ID:
chr2|113957993|113982064|-
Gene name:
Aqr
Transcript:
ENSMUST00000043160.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
21,20,19,18,17,16,15,14,13,12,11,10
Exon/intron offsets:
0,3022,5454,7139,9357,13356,15475,18115,19376,21426,22886,24006
Genomic length:
24071
Exon/intron sizes in nt:
147,242,170,168,179,142,121,103,104,114,117,65
Flanking exon/introns:
chr2:113957506-113957993|chr2:113982064-113985445
Splice length:
1672
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGCCTGTCACCATGGACAAGGTTCATTACTGTGAAAGATTCATCGAACTGATGATTGATCTAGAGGCTCTACTCCCCACGAGGCGCTGGTTTAACACCATCCTGGATGACTCCCACCTTCTGGTTCACTGCTACCTCTCCAGTCTTGTTCACAGAGAAGAGGACGGCCATCTGTTTTCCCAGCTTTTAGACATGCTGAAGTTCTATACTGGCTTTGAGATTAACGACCAGACTGGAAATGCTCTGACAGAGAACGAGATGACCACGATTCACTATGACAGAATTACATCTCTGCAGAGGGCTGCCTTCGCACACTTCCCCGAGCTCTATGACTTTGCCCTCTCCAATGTGGCAGAAGTAGATGCCCGAGATTCCTTGGTCAAGTTTTTTGGGCCTCTCAGTTCAAACACACTCCACCAGGTGGCATCATACCTCTGCCTCTTGCCAACGCTCCCTAAAAATGAAGACACAACTTTTGATAAAGAGTTTCTTCTAGAATTGCTGGTGTCTCGCCACGAACGCCGCATCTCTCAAATTCAGCAACTGAATCAGATGCCTTTGTATCCAACTGAGAAAATTATATGGGATGAGAACATCGTCCCTACTGAGTACTATTCTGGGGAAGGCTGCCTTGCTCTCCCCAAGTTGAATCTGCAGTTTTTGACTCTGCACGACTATCTCCTGAGGAACTTCAATCTCTTCCGCTTAGAGTCAACTTACGAAATTAGACAAGACATCGAGGACAGTGTCAGCAGAATGAAGCCCTGGCAGTCTGAGTATGGTGGCGTAGTGTTCGGTGGTTGGGCACGGATGGCCCAGCCCATTGTGGCTTTCACTGTAGTCGAGGTTGCCAAACCCAACATCGGTGAAAACTGGCCAACCCGAGTTCGTGCAGATGTCACCATCAATCTGAATGTCAGAGACCACATCAAGGATGAGTGGGAAGGTCTTCGAAAGCACGATGTATGCTTTTTAATTACTGTGCGTCCAACAAAACCTTACGGCACTAAGTTTGACAGGAGGAGACCTTTTATTGAGCAAGTTGGTCTGGTGTACGTCAGAGGCTGTGAAATCCAGGGCATGCTGGATGACAAAGGGCGAGTCATCGAAGACGGACCTGAACCCAGACCCAATCTTAGAGGAGAGTCAAGGACATTTCGAGTATTTTTGGATCCAAACCAGTATCAACAGGATATGACCAATACGATACAAAACGGAGCCGAAGATGTGTATGACACTTTCAATGTCATAATGAGGAGGAAACCGAAGGAGAATAACTTCAAGGCTGTGTTGGAGACGATTCGGAATCTGATGAATACTGACTGCGTGGTACCGGACTGGCTGCATGACATCATTTTGGGCTATGGGGACCCAAGTAGTGCCCATTATTCAAAAATGCCCAATCAGATTGCCACCCTGGATTTCAATGACACTTTTCTCTCCATTGAACATTTAAAGGCCAGCTTTCCTGGTCATAATGTCAAAGTGACTGTGAGTGACCCAGCACTCCAAATCCCCCCTTTCAGAATAACGTTCCCAGTAAGAAGTGGAAAGGGGAAGAAAAGAAAAGATGCAGACGGGGAAGAAGATGACACAGAAGAAGCAAAGACCTTAATTGTGGAGCCTCATGTTATTCCTAATAGAGGCCCTTATCCTTACAACCAGCCCAAACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACGGGGAAGAAGATGACACA
59.72
CGTGGGGAGTAGAGCCTCTA
59.82
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACGGGGAAGAAGATGACACAG
59.72
CGTGGGGAGTAGAGCCTCTA
59.82
177
PCR template sequence:
CAGACGGGGAAGAAGATGACACAGAAGAAGCAAAGACCTTAATTGTGGAGCCTCATGTTATTCCTAATAGAGGCCCTTATCCTTACAACCAGCCCAAACGAGCCTGTCACCATGGACAAGGTTCATTACTGTGAAAGATTCATCGAACTGATGATTGATCTAGAGGCTCTACTCCCCACGAGGCGCTGGTTTAACACCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
23
Interacting miRNAs (#binding sites)
mmu-miR-185-5p(3), mmu-miR-320-3p(2), mmu-let-7a-5p(1), mmu-miR-181a-5p(1), mmu-let-7b-5p(1), mmu-miR-31-5p(1), mmu-let-7c-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-139-5p(1), mmu-miR-181b-5p(1), mmu-let-7d-5p(1), mmu-miR-434-5p(1), mmu-miR-182-5p(1), mmu-miR-541-5p(1), mmu-miR-680(1), mmu-miR-673-5p(1), mmu-miR-1904(1), mmu-miR-574-5p(1), mmu-miR-338-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACCAGCCCAAACGAGCCTGTCACCAT
siRNA sets
TGGTGACAGGCTCGTTTGG, GGTGACAGGCTCGTTTGGG, ATGGTGACAGGCTCGTTTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAQR_1672_ORF_1
1.2251
0.244173274
0.99996764
1716
ORF sequence:
ATGTTATTCCTAATAGAGGCCCTTATCCTTACAACCAGCCCAAACGAGCCTGTCACCATGGACAAGGTTCATTACTGTGAAAGATTCATCGAACTGATGATTGATCTAGAGGCTCTACTCCCCACGAGGCGCTGGTTTAACACCATCCTGGATGACTCCCACCTTCTGGTTCACTGCTACCTCTCCAGTCTTGTTCACAGAGAAGAGGACGGCCATCTGTTTTCCCAGCTTTTAGACATGCTGAAGTTCTATACTGGCTTTGAGATTAACGACCAGACTGGAAATGCTCTGACAGAGAACGAGATGACCACGATTCACTATGACAGAATTACATCTCTGCAGAGGGCTGCCTTCGCACACTTCCCCGAGCTCTATGACTTTGCCCTCTCCAATGTGGCAGAAGTAGATGCCCGAGATTCCTTGGTCAAGTTTTTTGGGCCTCTCAGTTCAAACACACTCCACCAGGTGGCATCATACCTCTGCCTCTTGCCAACGCTCCCTAAAAATGAAGACACAACTTTTGATAAAGAGTTTCTTCTAGAATTGCTGGTGTCTCGCCACGAACGCCGCATCTCTCAAATTCAGCAACTGAATCAGATGCCTTTGTATCCAACTGAGAAAATTATATGGGATGAGAACATCGTCCCTACTGAGTACTATTCTGGGGAAGGCTGCCTTGCTCTCCCCAAGTTGAATCTGCAGTTTTTGACTCTGCACGACTATCTCCTGAGGAACTTCAATCTCTTCCGCTTAGAGTCAACTTACGAAATTAGACAAGACATCGAGGACAGTGTCAGCAGAATGAAGCCCTGGCAGTCTGAGTATGGTGGCGTAGTGTTCGGTGGTTGGGCACGGATGGCCCAGCCCATTGTGGCTTTCACTGTAGTCGAGGTTGCCAAACCCAACATCGGTGAAAACTGGCCAACCCGAGTTCGTGCAGATGTCACCATCAATCTGAATGTCAGAGACCACATCAAGGATGAGTGGGAAGGTCTTCGAAAGCACGATGTATGCTTTTTAATTACTGTGCGTCCAACAAAACCTTACGGCACTAAGTTTGACAGGAGGAGACCTTTTATTGAGCAAGTTGGTCTGGTGTACGTCAGAGGCTGTGAAATCCAGGGCATGCTGGATGACAAAGGGCGAGTCATCGAAGACGGACCTGAACCCAGACCCAATCTTAGAGGAGAGTCAAGGACATTTCGAGTATTTTTGGATCCAAACCAGTATCAACAGGATATGACCAATACGATACAAAACGGAGCCGAAGATGTGTATGACACTTTCAATGTCATAATGAGGAGGAAACCGAAGGAGAATAACTTCAAGGCTGTGTTGGAGACGATTCGGAATCTGATGAATACTGACTGCGTGGTACCGGACTGGCTGCATGACATCATTTTGGGCTATGGGGACCCAAGTAGTGCCCATTATTCAAAAATGCCCAATCAGATTGCCACCCTGGATTTCAATGACACTTTTCTCTCCATTGAACATTTAAAGGCCAGCTTTCCTGGTCATAATGTCAAAGTGACTGTGAGTGACCCAGCACTCCAAATCCCCCCTTTCAGAATAACGTTCCCAGTAAGAAGTGGAAAGGGGAAGAAAAGAAAAGATGCAGACGGGGAAGAAGATGACACAGAAGAAGCAAAGACCTTAATTGTGGAGCCTCATGTTATTCCTAATAGAGGCCCTTATCCTTACAACCAGCCCAAA
Peptide sequence:
MLFLIEALILTTSPNEPVTMDKVHYCERFIELMIDLEALLPTRRWFNTILDDSHLLVHCYLSSLVHREEDGHLFSQLLDMLKFYTGFEINDQTGNALTENEMTTIHYDRITSLQRAAFAHFPELYDFALSNVAEVDARDSLVKFFGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQIQQLNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLRNFNLFRLESTYEIRQDIEDSVSRMKPWQSEYGGVVFGGWARMAQPIVAFTVVEVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHDVCFLITVRPTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIEDGPEPRPNLRGESRTFRVFLDPNQYQQDMTNTIQNGAEDVYDTFNVIMRRKPKENNFKAVLETIRNLMNTDCVVPDWLHDIILGYGDPSSAHYSKMPNQIATLDFNDTFLSIEHLKASFPGHNVKVTVSDPALQIPPFRITFPVRSGKGKKRKDADGEEDDTEEAKTLIVEPHVIPNRGPYPYNQPK
IRESfinder result
IRESfinder score
0.484845178
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page