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mmu_circAp3b2_306
Basic information of circular RNA
PanCircBase ID:
mmu_circAp3b2_306
mm39_Chr. Position ID:
chr7|81122747|81123667|-
Gene name:
Ap3b2
Transcript:
ENSMUST00000082090.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
13,12,11
Exon/intron offsets:
0,387,857
Genomic length:
920
Exon/intron sizes in nt:
110,133,63
Flanking exon/introns:
chr7:81121746-81122747|chr7:81123667-81126027
Splice length:
306
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTGGAAGTGCTGACCAACCTGGCCAATGAGACTAACATCCCCACTGTTCTCCGGGAATTCCAGACCTACATTCGCAGCATGGACAAAGACTTTGTGGCAGCCACGATCCAGGCCATTGGACGATGTGCAACGAACATAGGCCGAGTCCGTGACACCTGTCTCAATGGCTTGGTGCAGCTTCTGTCCAACCGTGACGAGCTTGTGGTTGCAGAGTCAGTGGTGGTCATTAAGAAGCTGCTGCAAATGCAGCCAGCGCAGCACGGTGAGATCATCAAACACTTGGCAAAGCTCACAGACAACATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAGTCAGTGGTGGTCAT
59.68
TGCTGCGAATGTAGGTCTGG
60.11
178
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGCACGGTGAGATCATCA
60.46
AGTCTTTGTCCATGCTGCGA
59.96
143
PCR template sequence:
TGCAGAGTCAGTGGTGGTCATTAAGAAGCTGCTGCAAATGCAGCCAGCGCAGCACGGTGAGATCATCAAACACTTGGCAAAGCTCACAGACAACATCCAGCTGGAAGTGCTGACCAACCTGGCCAATGAGACTAACATCCCCACTGTTCTCCGGGAATTCCAGACCTACATTCGCAGCATGGACAAAGACTTTGTGGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AGACAACATCCAGCTGGAAGTGCTGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAP3B2_306_ORF_1
1.2335
0.522394506
0.953726645
534
ORF sequence:
ATGGACAAAGACTTTGTGGCAGCCACGATCCAGGCCATTGGACGATGTGCAACGAACATAGGCCGAGTCCGTGACACCTGTCTCAATGGCTTGGTGCAGCTTCTGTCCAACCGTGACGAGCTTGTGGTTGCAGAGTCAGTGGTGGTCATTAAGAAGCTGCTGCAAATGCAGCCAGCGCAGCACGGTGAGATCATCAAACACTTGGCAAAGCTCACAGACAACATCCAGCTGGAAGTGCTGACCAACCTGGCCAATGAGACTAACATCCCCACTGTTCTCCGGGAATTCCAGACCTACATTCGCAGCATGGACAAAGACTTTGTGGCAGCCACGATCCAGGCCATTGGACGATGTGCAACGAACATAGGCCGAGTCCGTGACACCTGTCTCAATGGCTTGGTGCAGCTTCTGTCCAACCGTGACGAGCTTGTGGTTGCAGAGTCAGTGGTGGTCATTAAGAAGCTGCTGCAAATGCAGCCAGCGCAGCACGGTGAGATCATCAAACACTTGGCAAAGCTCACAGACAACATCCAG
Peptide sequence:
MDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAKLTDNIQLEVLTNLANETNIPTVLREFQTYIRSMDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAKLTDNIQ
IRESfinder result
IRESfinder score
0.370309543
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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