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mmu_circAp2a2_668
Basic information of circular RNA
PanCircBase ID:
mmu_circAp2a2_668
mm39_Chr. Position ID:
chr7|141200384|141203694|+
Gene name:
Ap2a2
Transcript:
ENSMUST00000003038.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
12,13,14,15
Exon/intron offsets:
0,320,643,3146
Genomic length:
3310
Exon/intron sizes in nt:
98,23,21,74,164
Flanking exon/introns:
chr7:141199546-141200384|chr7:141203694-141206020
Splice length:
668
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCATTGCAGGCGCCAGCATGTCATGAGAACTTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGGGACCCACGATCCAGCCCTCTGATCCAGTTCAACCTGCTCCACTCCAAGTTCCACTTGTGCAGCGTCCCCACTCGGGCACTCCTGCTGTCCACCTACATCAAGTTCGTGAACCTCTTTCCAGAGGTAAAAGCTACCATTCAGGACGTGCTGCGCAGTGACAGCCAGCTGAAGAATGCCGACGTGGAGCTGCAGCAGCGGGCTGTGGAGTACCTGCGACTGAGCACTGTCGCCAGCACTGACATCCTGGCAACTGTCCTGGAGGAAATGCCACCCTTCCCTGAGCGTGAGTCCTCCATCTTGGCCAAGCTGAAGAAGAAGAAGGGCCCAAGCACAGTGACTGACCTGGAGGAGACCAAGCGGGAGCGAAGCATCGATGTGAATGGGGGCCCTGAACCCGTTCCAGCCAGCACCAGTGCTGCGTCCACACCTTCTCCGTCAGCAGACCTGCTGGGTCTGGGGGCCGTGCCCCCTGCTCCCACGGGGCCCCCTCCCTCCTCTGGCGGCGGGCTGCTGGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTCGCACCTCTTGCACCCGGCTCTGAAGACAACTTTGCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGACTCAGCTTCTGCAGTCG
59.76
TGGGTCCCCAGCTATCAAGT
60.25
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTGGACGTGTTCTCAGAC
59.61
TGGGTCCCCAGCTATCAAGT
60.25
163
PCR template sequence:
CCCCTCCCTCCTCTGGCGGCGGGCTGCTGGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTCGCACCTCTTGCACCCGGCTCTGAAGACAACTTTGCCAGGCATTGCAGGCGCCAGCATGTCATGAGAACTTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGATAGCTGGGGACCCACGATCCAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-296-5p(1), mmu-miR-320-3p(1), mmu-miR-136-5p(1), mmu-miR-676-3p(1), mmu-miR-383-5p(1), mmu-miR-705(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACAACTTTGCCAGGCATTGCAGGCGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAP2A2_668_ORF_1
0.8661
0.306312779
0.609764599
396
ORF sequence:
ATGCCACCCTTCCCTGAGCGTGAGTCCTCCATCTTGGCCAAGCTGAAGAAGAAGAAGGGCCCAAGCACAGTGACTGACCTGGAGGAGACCAAGCGGGAGCGAAGCATCGATGTGAATGGGGGCCCTGAACCCGTTCCAGCCAGCACCAGTGCTGCGTCCACACCTTCTCCGTCAGCAGACCTGCTGGGTCTGGGGGCCGTGCCCCCTGCTCCCACGGGGCCCCCTCCCTCCTCTGGCGGCGGGCTGCTGGTGGACGTGTTCTCAGACTCAGCTTCTGCAGTCGCACCTCTTGCACCCGGCTCTGAAGACAACTTTGCCAGGCATTGCAGGCGCCAGCATGTCATGAGAACTTGGTCAAAGTGGGCGGCTACATCCTGGGGGAGTTTGGAAACTTGA
Peptide sequence:
MPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARHCRRQHVMRTWSKWAATSWGSLET
IRESfinder result
IRESfinder score
0.429140788
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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