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mmu_circAp1g1_765
Basic information of circular RNA
PanCircBase ID:
mmu_circAp1g1_765
mm39_Chr. Position ID:
chr8|110559348|110576375|+
Gene name:
Ap1g1
Transcript:
ENSMUST00000093157.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
8,9,10,11,12,13,14,15
Exon/intron offsets:
0,473,751,5026,6202,10636,11608,16904
Genomic length:
17027
Exon/intron sizes in nt:
96,81,99,56,11,41,41,50,000
Flanking exon/introns:
chr8:110557253-110559348|chr8:110576375-110577672
Splice length:
765
Other database IDs:
circAtlas: mmu-Ap1g1_0035
Human Ortholog IDs:
Mature spliced Sequence:
CTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGATATTCCCCAGAACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGGACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACAGAGACTAGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAATCAGAGAGTGGATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATATTAGATATGTTGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGAAGCACAATTGTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTGCCCTGGTGAATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCCAGAATTTAAAGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGGCATATAGATACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACTTGATTCAGTTAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCTCGGTGACTATTCTCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGGAGATGCACGCTTACA
59.68
ACCTGCAAAAAGGGGTCACT
59.74
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGATGCACGCTTACACC
60.39
ACCTGCAAAAAGGGGTCACT
59.74
160
PCR template sequence:
TGCAGTTCCTAACTTGATTCAGTTAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCTCGGTGACTATTCTCAACTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGATATTCCCCAGAACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-217-5p(1), mmu-miR-153-3p(1), mmu-miR-676-3p(1), mmu-miR-382-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGACTATTCTCAACTTGTGCCCCAAT
siRNA sets
ATTGGGGCACAAGTTGAGA, TTGGGGCACAAGTTGAGAA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAP1G1_765_ORF_1
0.9588
0.107555655
0.999548936
1491
ORF sequence:
ATGTCCGGATATTCCCCAGAACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGGACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACAGAGACTAGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAATCAGAGAGTGGATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATATTAGATATGTTGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGAAGCACAATTGTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTGCCCTGGTGAATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCCAGAATTTAAAGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGGCATATAGATACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACTTGATTCAGTTAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCTCGGTGACTATTCTCAACTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGATATTCCCCAGAACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGGACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACAGAGACTAGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAATCAGAGAGTGGATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATATTAGATATGTTGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGAAGCACAATTGTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTGCCCTGGTGAATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCCAGAATTTAAAGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGGCATATAGATACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACTTGATTCAGTTAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCTCGGTGACTATTCTCAA
Peptide sequence:
MSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQ
IRESfinder result
IRESfinder score
0.511689838
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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