top of page
mmu_circAnxa4_761
Basic information of circular RNA
PanCircBase ID:
mmu_circAnxa4_761
mm39_Chr. Position ID:
chr6|86721606|86742789|-
Gene name:
Anxa4
Transcript:
ENSMUST00000001187.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,4262,6438,7528,9178,10228,13194,16049,21137
Genomic length:
21183
Exon/intron sizes in nt:
96,94,57,80,91,114,95,88,46
Flanking exon/introns:
chr6:86720158-86721606|chr6:86742789-86770490
Splice length:
761
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAACTTCTGCAGGTGTGACTGAACTCTGAACGTGAGCTACAGCATGGAAGCCAAAGGAGGAACCGTGAAAGCTGCTTCAGGATTCAACGCAACTGAAGATGCCCAGACCCTGAGGAAGGCCATGAAAGGCCTCGGTACTGATGAAGATGCCATTATCGGAATCTTAGCCTACAGAAACACCGCCCAGCGCCAGGAGATCAGGAGTGCCTACAAGAGCACCATTGGCAGGGACCTGATTGAGGACTTGAAGTCGGAGCTGAGCAGCAACTTTGAGCAGGTGATCCTGGGGCTGATGACACCCACAGTGCTGTATGACGTACAGGAGCTGCGGAGGGCCATGAAGGGAGCTGGCACGGACGAGGGCTGTCTGATTGAGATCCTGGCCTCTCGTACCCCTGAGGAGATCCGTCGAATCAACCAGACATACCAGCAGCAATATGGAAGGAGCCTTGAAGAGGACATCTGCTCTGACACATCCTTCATGTTCCAGCGAGTACTGGTGTCCCTGTCGGCGGCTGGCAGGGATGAAGGAAATTACCTTGATGATGCTCTTATGAAGCAGGACGCCCAGGAACTGTATGAGGCTGGAGAGAAGAGATGGGGGACAGACGAGGTGAAATTCCTAAGCATTCTCTGTTCCCGGAACCGGAACCATCTGCTCCACGTGTTTGATGAATACAAAAGAATCTCACAGAAGGACATTGAACAGAGCATTAAGTCTGAGACATCTGGAAGCTTTGAAGATGCCCTGTTGGCTATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGAAGGACATTGAACAGAGCA
60.18
CAGCTTTCACGGTTCCTCCT
59.96
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACAGAAGGACATTGAACAGAGC
59.81
CAGCTTTCACGGTTCCTCCT
59.96
146
PCR template sequence:
CACGTGTTTGATGAATACAAAAGAATCTCACAGAAGGACATTGAACAGAGCATTAAGTCTGAGACATCTGGAAGCTTTGAAGATGCCCTGTTGGCTATAGGAACTTCTGCAGGTGTGACTGAACTCTGAACGTGAGCTACAGCATGGAAGCCAAAGGAGGAACCGTGAAAGCTGCTTCAGGATTCAACGCAACTGAAGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-205-5p(2), mmu-miR-9-5p(1), mmu-miR-211-5p(1), mmu-miR-328-3p(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTGTTGGCTATAGGAACTTCTGCAGG
siRNA sets
AAGTTCCTATAGCCAACAG, CTGCAGAAGTTCCTATAGC, TGCAGAAGTTCCTATAGCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANXA4_761_ORF_1
1.1571
0.442302879
0.983243793
747
ORF sequence:
ATGGAAGCCAAAGGAGGAACCGTGAAAGCTGCTTCAGGATTCAACGCAACTGAAGATGCCCAGACCCTGAGGAAGGCCATGAAAGGCCTCGGTACTGATGAAGATGCCATTATCGGAATCTTAGCCTACAGAAACACCGCCCAGCGCCAGGAGATCAGGAGTGCCTACAAGAGCACCATTGGCAGGGACCTGATTGAGGACTTGAAGTCGGAGCTGAGCAGCAACTTTGAGCAGGTGATCCTGGGGCTGATGACACCCACAGTGCTGTATGACGTACAGGAGCTGCGGAGGGCCATGAAGGGAGCTGGCACGGACGAGGGCTGTCTGATTGAGATCCTGGCCTCTCGTACCCCTGAGGAGATCCGTCGAATCAACCAGACATACCAGCAGCAATATGGAAGGAGCCTTGAAGAGGACATCTGCTCTGACACATCCTTCATGTTCCAGCGAGTACTGGTGTCCCTGTCGGCGGCTGGCAGGGATGAAGGAAATTACCTTGATGATGCTCTTATGAAGCAGGACGCCCAGGAACTGTATGAGGCTGGAGAGAAGAGATGGGGGACAGACGAGGTGAAATTCCTAAGCATTCTCTGTTCCCGGAACCGGAACCATCTGCTCCACGTGTTTGATGAATACAAAAGAATCTCACAGAAGGACATTGAACAGAGCATTAAGTCTGAGACATCTGGAAGCTTTGAAGATGCCCTGTTGGCTATAGGAACTTCTGCAGGTGTGACTGAACTCTGA
Peptide sequence:
MEAKGGTVKAASGFNATEDAQTLRKAMKGLGTDEDAIIGILAYRNTAQRQEIRSAYKSTIGRDLIEDLKSELSSNFEQVILGLMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRINQTYQQQYGRSLEEDICSDTSFMFQRVLVSLSAAGRDEGNYLDDALMKQDAQELYEAGEKRWGTDEVKFLSILCSRNRNHLLHVFDEYKRISQKDIEQSIKSETSGSFEDALLAIGTSAGVTEL
IRESfinder result
IRESfinder score
0.247129155
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page