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mmu_circAnp32e_330
Basic information of circular RNA
PanCircBase ID:
mmu_circAnp32e_330
mm39_Chr. Position ID:
chr3|95844335|95845351|+
Gene name:
Anp32e
Transcript:
ENSMUST00000165307.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,5
Exon/intron offsets:
0,858
Genomic length:
1016
Exon/intron sizes in nt:
1,72,158
Flanking exon/introns:
chr3:95842210-95844335|chr3:95845351-95851618
Splice length:
330
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAAAATCTTAAAAATTTGAAAAGTCTCGACTTGTTTAACTGTGAGATCACAAACCTGGAAGATTATAGAGAAAGTATTTTTGAACTGCTGCAGCAGATCACCTACCTGGACGGATTCGACCAGGAGGACAACGAAGCTCCCGACTCGGAGGAGGAGGATGACGACGACGAGGATGGAGATGAAGATGAGGAAGATGAGGACGAAGATGAAGCTGGCCCACCGGAAGGCTATGAAGAGGAGGAAGATGACGATGAGGATGAAGCTGGCTCAGAAGTGGGAGAGGGAGAAGAGGAGGTGGGCCTCTCATACTTAATGAAAGACGAAATTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGCTGGCTCAGAAGTGG
59.96
GCTGCAGCAGTTCAAAAATACT
58.09
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGGAGAGGGAGAAGAGGAGG
60.11
GCTGCAGCAGTTCAAAAATACT
58.09
149
PCR template sequence:
TGAAGAGGAGGAAGATGACGATGAGGATGAAGCTGGCTCAGAAGTGGGAGAGGGAGAAGAGGAGGTGGGCCTCTCATACTTAATGAAAGACGAAATTCAGCAAAATCTTAAAAATTTGAAAAGTCTCGACTTGTTTAACTGTGAGATCACAAACCTGGAAGATTATAGAGAAAGTATTTTTGAACTGCTGCAGCAGATCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-127-3p(1), mmu-miR-207(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AGACGAAATTCAGCAAAATCTTAAAA
siRNA sets
TTTTAAGATTTTGCTGAAT, ATTTTGCTGAATTTCGTCT, TTTAAGATTTTGCTGAATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANP32E_330_ORF_1
1.2229
0.662915658
0.903545988
348
ORF sequence:
ATGAAAGACGAAATTCAGCAAAATCTTAAAAATTTGAAAAGTCTCGACTTGTTTAACTGTGAGATCACAAACCTGGAAGATTATAGAGAAAGTATTTTTGAACTGCTGCAGCAGATCACCTACCTGGACGGATTCGACCAGGAGGACAACGAAGCTCCCGACTCGGAGGAGGAGGATGACGACGACGAGGATGGAGATGAAGATGAGGAAGATGAGGACGAAGATGAAGCTGGCCCACCGGAAGGCTATGAAGAGGAGGAAGATGACGATGAGGATGAAGCTGGCTCAGAAGTGGGAGAGGGAGAAGAGGAGGTGGGCCTCTCATACTTAATGAAAGACGAAATTCAG
Peptide sequence:
MKDEIQQNLKNLKSLDLFNCEITNLEDYRESIFELLQQITYLDGFDQEDNEAPDSEEEDDDDEDGDEDEEDEDEDEAGPPEGYEEEEDDDEDEAGSEVGEGEEEVGLSYLMKDEIQ
IRESfinder result
IRESfinder score
0.199180861
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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