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mmu_circAno6_563
Basic information of circular RNA
PanCircBase ID:
mmu_circAno6_563
mm39_Chr. Position ID:
chr15|95762105|95811537|+
Gene name:
Ano6
Transcript:
ENSMUST00000071874.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
2,3,4,5
Exon/intron offsets:
0,30188,48080,49147
Genomic length:
49432
Exon/intron sizes in nt:
8,31,29,66,285
Flanking exon/introns:
chr15:95688937-95762105|chr15:95811537-95811752
Splice length:
563
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACTTCAGGACTCCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGGCGAATCGACTTCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAGAAACAGAAGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACAAGATCTGTTTCTGATGACAAGCTTGTGTTCGTAAAAGTGCACGCGCCCTGGGAAGTGCTGTGCACCTATGCTGAGATCATGCACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAACCTCAACTGGTTCACCAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACTGCCCCTTTTGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAGCCGCATT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCCTTTTGAGAAGAGCC
60.25
TGGAGTCCTGAAGTCCTGCT
60.18
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCCTTTTGAGAAGAGCC
60.25
GGAGTCCTGAAGTCCTGCTG
59.75
161
PCR template sequence:
GCAGGAGTTCTTCACTGCCCCTTTTGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAGCCGCATTTGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACTTCAGGACTCCAGAGTTTGAAGAATTTAACGGGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-211-5p(1), mmu-miR-425-5p(1), mmu-miR-129-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGAAGCCGCATTTGCTGGAAAACTT
siRNA sets
GTTTTCCAGCAAATGCGGC, AAGTTTTCCAGCAAATGCG, TTTTCCAGCAAATGCGGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANO6_563_ORF_1
0.9709
0.098216803
0.664393607
519
ORF sequence:
ATGCACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAACCTCAACTGGTTCACCAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACTGCCCCTTTTGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAGCCGCATTTGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACTTCAGGACTCCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGGCGAATCGACTTCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAGAAACAGAAGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACAAGATCTGTTTCTGATGACAAGCTTGTGTTCGTAA
Peptide sequence:
MHIKLPLKPNDLKTRSPFGNLNWFTKVLRVNESVIKPEQEFFTAPFEKSRMNDFYILDRDSFFNPATRSRICWKTLTRQLSAPPLDHWRISRTSGLQSLKNLTGSPTPSFSPMARGESTSSSCMKMRAKRRTIRKGQMRNRRGKDKHTNLTLSAMGCSWKQQDLFLMTSLCS
IRESfinder result
IRESfinder score
0.530981778
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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